2. Immunology/Inflammation Apoptosis Stem Cell/Wnt MAPK/ERK Pathway
  3. NO Synthase Apoptosis ERK
  4. Aminoguanidine hydrochloride

アミノグアニジン 塩酸塩  (Synonyms: Aminoguanidine hydrochloride; 塩酸アミノグアニジン; Pimagedine hydrochloride; GER-11; Aminoguanidinium chloride)

製品番号: HY-B1041 純度: 98.0%
COA 取扱説明書 Technical Support

Aminoguanidine (Pimagedine) hydrochloride is an inhibitor of diamine oxidase (DAO) and nitric oxide synthase (NOS). Aminoguanidine hydrochloride can reduce the formation of advanced glycation end products (AGEs). Aminoguanidine hydrochloride has a dose-dependent inhibitory effect on Doxorubicin (HY-15142)-induced cell apoptosis. Aminoguanidine hydrochloride has antioxidant properties. Aminoguanidine hydrochloride can be used in the research of diabetic nephropathy.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 1937-19-5

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 30 在庫あり
Solution
10 mM * 1 mL in DMSO USD 30 在庫あり
Solid
25 mg $28 在庫あり
50 mg $44 在庫あり
100 mg $66 在庫あり
500 mg $100 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 9 publication(s) in Google Scholar

Other Forms of Aminoguanidine hydrochloride:

アミノグアニジン 塩酸塩 関連抗体

Top Publications Citing Use of Products

    Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 21;59(41):21898-21909.  [Abstract]

    Western blot analysis of intracellular levels of the MG-H1 following 24-hour treatment with: 0.5% DMSO (control), 25 μM BPS, 50 μM BPS, 400 μM aminoguanidine + 25 μM BPS, and 400 μM aminoguanidine hydrochloride + 50 μM BPS.

    Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2024 Dec:197:107046.  [Abstract]

    BMDMs pretreated with degarelix (1 μM), aminoguanidine hydrochloride (AGHS, 100 μM), BafA1 (100 nM), NAC (5 mM), Z-VAD (20 μM), or 3-MA (5 mM) were infected with H37Rv (MOI = 2). At different time points after infection, bacterial survival was calculated by dividing the CFU at 24 h by the CFU at 3 h.

    Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 May 17;151:113109.  [Abstract]

    Immunofluorescence analysis showed that the expression of Gli1 protein differed between the high glucose group and the high glucose plus Aminoguanidine (Ami, 500 μM, 24 h) group in organotypic cultured mouse MGs.

    Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec;120:109527.  [Abstract]

    Treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks), ALA , or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) significantly reduced the RAGE and 3‑NT levels in ZDF rats.

    Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec;120:109527.  [Abstract]

    The protein expression of the NADPH oxidase subunits NOX2 and p22phox was significantly increased in the ZDF rats, while treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks) or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) specifically reduced the NOX2 and p22phox levels in the ZDF rats.

    NO Synthase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    nNOS iNOS eNOS

    ERK アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    ERK ERK1 ERK2 ERK5 ERK8

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Aminoguanidine (Pimagedine) hydrochloride is an inhibitor of diamine oxidase (DAO) and nitric oxide synthase (NOS). Aminoguanidine hydrochloride can reduce the formation of advanced glycation end products (AGEs). Aminoguanidine hydrochloride has a dose-dependent inhibitory effect on Doxorubicin (HY-15142)-induced cell apoptosis. Aminoguanidine hydrochloride has antioxidant properties. Aminoguanidine hydrochloride can be used in the research of diabetic nephropathy[1][2][3][4][5].

    Cellular Effect
    Cell Line Type Value Description References
    RAW264.7 IC50
    26.2 3
    Compound: AG
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    		[PMID: 29924604]
    RAW264.7 IC50
    26.2 3
    Compound: AG
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    		[PMID: 29924604]
    RAW264.7 IC50
    26.2 3
    Compound: AG
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    		[PMID: 29924604]
    体外実験

    Aminoguanidine (100, 200, 500, 1000 μM, 24 h) can reduce DOX-induced DNA damage and apoptosis in A549 cells [1].
    Aminoguanidine (100 μM, 30 min) can induce ERK activation in AR42J cells and promote cell proliferation [2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    アミノグアニジン 塩酸塩 関連抗体

    Apoptosis Analysis [1]

    Cell Line: A549
    Concentration: 100-1000 μM
    Incubation Time: 24 h
    Result: Showed protective effect on DOX-induced DNA damage and decreased DOX-induced apoptosis.

    Cell Proliferation Assay [2]

    Cell Line: AR42J
    Concentration: 100 μM
    Incubation Time: 24-96 h
    Result: Showed a significant increase in cell proliferation after incubation for 48 h.
    体内実験

    minoguanidine (50 mg/kg, Intraperitoneal injection ) protects mice from CCl4-induced hepatotoxicity[3].
    Aminoguanidine (200 mg/kg, Single dose intraperitoneal injection) is protective against cyclophosphamide (CP) -induced oxidative stress and kidney damage in rats[4].br/>

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Swiss albino mice[3]
    Dosage: 50 mg/kg
    Administration: Intraperitoneally 30 min before the administration of CCl4
    Result: Inhibited the serum AST level and protected hepatotoxin-induced lipid peroxidation.
    Animal Model: Adult male Wistar rats[4]
    Dosage: 200 mg/kg
    Administration: Intraperitoneally 1 hour before the administration of CP and killed 16 hours after CP injection.
    Result: Attenuated CP-induced MDA elevation and prevented CP-induced protein oxidation.
    Restored the GSH levels and attenuated CP-induced increase in MPO activity.
    分子量

    110.55

    分子式

    CH7ClN4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NNC(N)=N.[H]Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 100 mg/mL (904.57 mM)

    DMSO : 100 mg/mL (904.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 9.0457 mL 45.2284 mL 90.4568 mL
    5 mM 1.8091 mL 9.0457 mL 18.0914 mL
    10 mM 0.9046 mL 4.5228 mL 9.0457 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (22.61 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (22.61 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (904.57 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: ≥98.0%

    COA (245 KB) HNMR (119 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 9.0457 mL 45.2284 mL 90.4568 mL 226.1420 mL
    5 mM 1.8091 mL 9.0457 mL 18.0914 mL 45.2284 mL
    10 mM 0.9046 mL 4.5228 mL 9.0457 mL 22.6142 mL
    15 mM 0.6030 mL 3.0152 mL 6.0305 mL 15.0761 mL
    20 mM 0.4523 mL 2.2614 mL 4.5228 mL 11.3071 mL
    25 mM 0.3618 mL 1.8091 mL 3.6183 mL 9.0457 mL
    30 mM 0.3015 mL 1.5076 mL 3.0152 mL 7.5381 mL
    40 mM 0.2261 mL 1.1307 mL 2.2614 mL 5.6536 mL
    50 mM 0.1809 mL 0.9046 mL 1.8091 mL 4.5228 mL
    60 mM 0.1508 mL 0.7538 mL 1.5076 mL 3.7690 mL
    80 mM 0.1131 mL 0.5654 mL 1.1307 mL 2.8268 mL
    100 mM 0.0905 mL 0.4523 mL 0.9046 mL 2.2614 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Aminoguanidine hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Aminoguanidine1937-19-5Pimagedine GER-11AminoguanidiniumGER11GER 11GER-11NO SynthaseApoptosisERKNitric oxide synthasesNOSExtracellular signal regulated kinasesInhibitorA549AR42J cellinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Aminoguanidine hydrochloride
    製品番号:
    HY-B1041
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.