2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS)
  4. Aminoguanidine hemisulfate

Aminoguanidine hemisulfate is an inhibitor of NOS and ROS. Aminoguanidine hemisulfate abolishes ANE-induced ROS production in vitro. Aminoguanidine hemisulfate is used for cancer research.

For research use only. We do not sell to patients.

CAS No. : 996-19-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
500 mg In-stock
1 g In-stock
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10 g   Get quote  

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Aminoguanidine hemisulfate:

Aminoguanidine hemisulfate Related Antibodies

Top Publications Citing Use of Products

    Aminoguanidine hemisulfate purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 21;59(41):21898-21909.  [Abstract]

    Western blot analysis of intracellular levels of the MG-H1 following 24-hour treatment with: 0.5% DMSO (control), 25 μM BPS, 50 μM BPS, 400 μM aminoguanidine + 25 μM BPS, and 400 μM aminoguanidine hydrochloride + 50 μM BPS.

    Aminoguanidine hemisulfate purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2024 Dec:197:107046.  [Abstract]

    BMDMs pretreated with degarelix (1 μM), aminoguanidine hydrochloride (AGHS, 100 μM), BafA1 (100 nM), NAC (5 mM), Z-VAD (20 μM), or 3-MA (5 mM) were infected with H37Rv (MOI = 2). At different time points after infection, bacterial survival was calculated by dividing the CFU at 24 h by the CFU at 3 h.

    Aminoguanidine hemisulfate purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 May 17;151:113109.  [Abstract]

    Immunofluorescence analysis showed that the expression of Gli1 protein differed between the high glucose group and the high glucose plus Aminoguanidine (Ami, 500 μM, 24 h) group in organotypic cultured mouse MGs.

    Aminoguanidine hemisulfate purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec;120:109527.  [Abstract]

    Treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks), ALA , or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) significantly reduced the RAGE and 3‑NT levels in ZDF rats.

    Aminoguanidine hemisulfate purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec;120:109527.  [Abstract]

    The protein expression of the NADPH oxidase subunits NOX2 and p22phox was significantly increased in the ZDF rats, while treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks) or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) specifically reduced the NOX2 and p22phox levels in the ZDF rats.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Aminoguanidine hemisulfate is an inhibitor of NOS and ROS. Aminoguanidine hemisulfate abolishes ANE-induced ROS production in vitro. Aminoguanidine hemisulfate is used for cancer research[1].

    IC50 & Target

    IC50: ROS, NOS[1]
    Molecular Weight

    123.13

    Formula

    CH6N4.1/2H2O4
    CAS No.
    Appearance

    Solid

    Color

    White to pink

    SMILES

    O=S(O)(O)=O.N=C(NN)N.[1/2]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (812.15 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.1215 mL 40.6075 mL 81.2150 mL
    5 mM 1.6243 mL 8.1215 mL 16.2430 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.01%

    SDS (393 KB)

    COA (250 KB) RP-HPLC (258 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 8.1215 mL 40.6075 mL 81.2150 mL 203.0374 mL
    5 mM 1.6243 mL 8.1215 mL 16.2430 mL 40.6075 mL
    10 mM 0.8121 mL 4.0607 mL 8.1215 mL 20.3037 mL
    15 mM 0.5414 mL 2.7072 mL 5.4143 mL 13.5358 mL
    20 mM 0.4061 mL 2.0304 mL 4.0607 mL 10.1519 mL
    25 mM 0.3249 mL 1.6243 mL 3.2486 mL 8.1215 mL
    30 mM 0.2707 mL 1.3536 mL 2.7072 mL 6.7679 mL
    40 mM 0.2030 mL 1.0152 mL 2.0304 mL 5.0759 mL
    50 mM 0.1624 mL 0.8121 mL 1.6243 mL 4.0607 mL
    60 mM 0.1354 mL 0.6768 mL 1.3536 mL 3.3840 mL
    80 mM 0.1015 mL 0.5076 mL 1.0152 mL 2.5380 mL
    100 mM 0.0812 mL 0.4061 mL 0.8121 mL 2.0304 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Aminoguanidine hemisulfate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Aminoguanidine hemisulfate996-19-0Reactive Oxygen Species (ROS)ANEROSapoptosisautophagyhepatocellular carcinomalysosomemytilus coruscusRNA interferencelarval metamorphosismarine environmental factorsnitric oxideInhibitorinhibitorinhibit

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