Cat. No.: HY-B1041

Aminoguanidine hydrochloride

(Synonyms: Pimagedine hydrochloride; GER-11; Aminoguanidinium hydrochloride)

10 Cited Publications

Customer Review

Based on 10 publication(s) in Google Scholar

Aminoguanidine (Pimagedine) hydrochloride is an inhibitor of diamine oxidase (DAO) and nitric oxide synthase (NOS). Aminoguanidine hydrochloride can reduce the formation of advanced glycation end products (AGEs). Aminoguanidine hydrochloride has a dose-dependent inhibitory effect on Doxorubicin (HY-15142)-induced cell apoptosis. Aminoguanidine hydrochloride has antioxidant properties. Aminoguanidine hydrochloride can be used in the research of diabetic nephropathy.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 1937-19-5
Formula: CH₇ClN₄
Molecular Weight:110.55

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Aminoguanidine hydrochloride Chemical Structure

CAS No.: 1937-19-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (10)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All NO Synthase Isoforms :

All ERK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	26.2 μM Compound: AG	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay	[PMID: 29924604]

In Vitro

Aminoguanidine (100, 200, 500, 1000 μM, 24 h) hydrochloride can reduce DOX-induced DNA damage and apoptosis in A549 cells ^[1].
Aminoguanidine (100 μM, 30 min) hydrochloride can induce ERK activation in AR42J cells and promote cell proliferation ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis ^[1]

Cell Line:

A549
Concentration:

100-1000 μM
Incubation Time:

24 h
Result:

Showed protective effect on DOX-induced DNA damage and decreased DOX-induced apoptosis.

Cell Proliferation Assay ^[2]

Cell Line:

AR42J
Concentration:

100 μM
Incubation Time:

24-96 h
Result:

Showed a significant increase in cell proliferation after incubation for 48 h.

In Vivo

minoguanidine (50 mg/kg, Intraperitoneal injection ) hydrochloride protects mice from CCl4-induced hepatotoxicity^[3].
Aminoguanidine (200 mg/kg, Single dose intraperitoneal injection) hydrochloride is protective against cyclophosphamide (CP) (HY-17420)-induced oxidative stress and kidney damage in rats^[4].br/>

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Swiss albino mice^[3]
Dosage:

50 mg/kg
Administration:

Intraperitoneally 30 min before the administration of CCl4
Result:

Inhibited the serum AST level and protected hepatotoxin-induced lipid peroxidation.

Animal Model:

Adult male Wistar rats^[4]
Dosage:

200 mg/kg
Administration:

Intraperitoneally 1 hour before the administration of CP and killed 16 hours after CP injection.
Result:

Attenuated CP-induced MDA elevation and prevented CP-induced protein oxidation.
Restored the GSH levels and attenuated CP-induced increase in MPO activity.

Chemical Information

CAS No. 1937-19-5
Appearance Solid
Molecular Weight 110.55
Formula CH₇ClN₄
Color White to off-white
SMILES

NNC(N)=N.Cl
Synonyms

Pimagedine hydrochloride; GER-11; Aminoguanidinium hydrochloride
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (10)

All WBIFIHCCell Proliferation/Viability Assay

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Journal Impact Factor

Nat Microbiol

Mycobacterium tuberculosis-derived linoleic acid increases regulatory T cell function to promote bacterial survival within macrophages. [Abstract]

2025 Nov;10(11):2949-2965. PMID: 41073667
Nat Commun

Targeting polyamine metabolism and ferroptosis enhances the efficacy of KRAS-targeted therapy depending on KEAP1 status. [Abstract]

2025 Nov 11;16(1):9923. PMID: 41219185
Adv Sci (Weinh)

Activating the Osteoblastic USP26 Pathway Alleviates Multi-Organ Fibrosis by Decreasing Insulin Resistance. [Abstract]

2025 Dec 19:e12424. PMID: 41417635
Environ Sci Technol

Revealing Ferroptosis Induction by Bisphenol A and Bisphenol S through Distinct Protein Targets. [Abstract]

2025 Oct 21;59(41):21898-21909. PMID: 41068997

Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 21;59(41):21898-21909. [Abstract]

Western blot analysis of intracellular levels of the MG-H1 following 24-hour treatment with: 0.5% DMSO (control), 25 μM BPS, 50 μM BPS, 400 μM aminoguanidine + 25 μM BPS, and 400 μM aminoguanidine hydrochloride + 50 μM BPS.
Clin Immunol

PLK2 facilitates Mycobacterium tuberculosis clearance via p38 MAPK-mediated ROS production. [Abstract]

2026 Jun:285:110705. PMID: 41962814
Skelet Muscle

Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice. [Abstract]

2025 Jan 13;15(1):2. PMID: 39806512
Microb Pathog

Degarelix limits the survival of mycobacteria and granuloma formation. [Abstract]

2024 Dec:197:107046. PMID: 39433139

Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2024 Dec:197:107046. [Abstract]

BMDMs pretreated with degarelix (1 μM), aminoguanidine hydrochloride (AGHS, 100 μM), BafA1 (100 nM), NAC (5 mM), Z-VAD (20 μM), or 3-MA (5 mM) were infected with H37Rv (MOI = 2). At different time points after infection, bacterial survival was calculated by dividing the CFU at 24 h by the CFU at 3 h.
SSRN

2024 May 22.
Biomed Pharmacother

Inhibition of Gli1 suppressed hyperglycemia-induced meibomian gland dysfunction by promoting pparγ expression. [Abstract]

2022 May 17;151:113109. PMID: 35594713

Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2022 May 17;151:113109. [Abstract]

Immunofluorescence analysis showed that the expression of Gli1 protein differed between the high glucose group and the high glucose plus Aminoguanidine (Ami, 500 μM, 24 h) group in organotypic cultured mouse MGs.
Biomed Pharmacother

AGEs impair Kv channel-mediated vasodilation of coronary arteries by activating the NF-κB signaling pathway in ZDF rats. [Abstract]

2019 Dec:120:109527. PMID: 31629953

Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec:120:109527. [Abstract]

Treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks), ALA , or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) significantly reduced the RAGE and 3‑NT levels in ZDF rats.

Aminoguanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec:120:109527. [Abstract]

The protein expression of the NADPH oxidase subunits NOX2 and p22phox was significantly increased in the ZDF rats, while treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks) or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) specifically reduced the NOX2 and p22phox levels in the ZDF rats.

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (904.57 mM)

DMSO : 100 mg/mL (904.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

9.0457 mL

45.2284 mL

90.4568 mL

5 mM

1.8091 mL

9.0457 mL

18.0914 mL

10 mM

0.9046 mL

4.5228 mL

9.0457 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (22.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (22.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (22.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (904.57 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sabuncuoglu S. Antiapoptotic effect of aminoguanidine on doxorubicin-induced apoptosis. Mol Cell Biochem. 2014 Sep;394(1-2):129-35. [Content Brief]

[2]. Chowdhury P. Aminoguanidine (AG) Induces Induced both Pro- and Antioxidant Effect in AR42J Cells, a Rat Pancreatic Tumor Cell Line. Ann Clin Lab Sci. 2017 Sep;47(5):572-580. PMID: 29066484. [Content Brief]

[3]. Al-Shabanah OA, et al. Protective effect of aminoguanidine, a nitric oxide synthase inhibitor, against carbon tetrachloride induced hepatotoxicity in mice. Life Sci. 2000;66(3):265-70. [Content Brief]

[4]. Abraham P, et al. Protective effect of aminoguanidine against cyclophosphamide-induced oxidative stress and renal damage in rats. Redox Rep. 2011;16(1):8-14. [Content Brief]

[5]. Zou Z, et al. Inhibition of Gli1 suppressed hyperglycemia-induced meibomian gland dysfunction by promoting pparγ expression. Biomed Pharmacother. 2022 Jul;151:113109. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	9.0457 mL	45.2284 mL	90.4568 mL	226.1420 mL
	5 mM	1.8091 mL	9.0457 mL	18.0914 mL	45.2284 mL
	10 mM	0.9046 mL	4.5228 mL	9.0457 mL	22.6142 mL
	15 mM	0.6030 mL	3.0152 mL	6.0305 mL	15.0761 mL
	20 mM	0.4523 mL	2.2614 mL	4.5228 mL	11.3071 mL
	25 mM	0.3618 mL	1.8091 mL	3.6183 mL	9.0457 mL
	30 mM	0.3015 mL	1.5076 mL	3.0152 mL	7.5381 mL
	40 mM	0.2261 mL	1.1307 mL	2.2614 mL	5.6536 mL
	50 mM	0.1809 mL	0.9046 mL	1.8091 mL	4.5228 mL
	60 mM	0.1508 mL	0.7538 mL	1.5076 mL	3.7690 mL
	80 mM	0.1131 mL	0.5654 mL	1.1307 mL	2.8268 mL
	100 mM	0.0905 mL	0.4523 mL	0.9046 mL	2.2614 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Aminoguanidine hydrochloride

10 Cited Publications

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Aminoguanidine hydrochloride

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Publications Citing Use of MedChemExpress (MCE) Aminoguanidine hydrochloride

All NO Synthase Isoforms

All ERK Isoforms

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Aminoguanidine hydrochloride Related Classifications

HY-B1041 Related Classifications