Spermidine

Name: Spermidine
Brand: MedChemExpress
SKU: HY-B1776
Rating: 5.0 (19 reviews)

Cat. No.: HY-B1776 Purity: 99.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Spermidine maintains cell membrane stability, increases antioxidant enzymes activities, improving photosystem II (PSII), and relevant gene expression. Spermidine significantly decreases the H₂O₂ and O₂^.- contents.

For research use only. We do not sell to patients.

CAS No. : 124-20-9

Size	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 19 publication(s) in Google Scholar

Other Forms of Spermidine:

19 Publications Citing Use of MCE Spermidine

In Vivo Efficacy Study

: Spermidine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 8. [Abstract]; Spermidine (10 mg/kg; i.p.; starting 7 days prior to irradiation and continuing until 9 days post-irradiation). Immunofluorescence staining confirms significantly enhanced epithelial proliferation in the Spermidine-treated group by day 9 post-radiation. Quantification of PCNA+ basal cells before irradiation and for 9 days post-irradiation (n = 6 mice). Five random images per mouse were analyzed. Scale bar, 100 μm.

: Spermidine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Jan 7;37(1):87-103.e10. [Abstract]; Spermidine (40 mg/kg; i.p.; every 2 days 3 times). WT (left, n = 3) or GF (right, n = 5) mice are i.p. injected with Spermidine (40 mg/kg) every 2 days 3 times, and the AWR scores are determined on day 8.

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	> 100 μM Compound: Spd, spermidine	Cytotoxicity against CHO cells after 48 hrs by MTT assay Cytotoxicity against CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO	IC₅₀	> 100 μM Compound: Spd, spermidine	Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO-MG	IC₅₀	> 100 μM Compound: 2, Spermidine	Inhibition of cell growth in CHO-MG cells in the presence of DFMO Inhibition of cell growth in CHO-MG cells in the presence of DFMO	[PMID: 16392808]
HeLa	IC₅₀	100 μM Compound: spermidine	Ability to inhibit 50% growth of HeLa cells Ability to inhibit 50% growth of HeLa cells	[PMID: 1875339]
HeLa	IC₅₀	1500 μM Compound: spermidine	Ability to cause aggregation of purified HeLa cell DNA Ability to cause aggregation of purified HeLa cell DNA	[PMID: 1875339]
L1210	IC₅₀	> 100 μM Compound: 7	Growth inhibitory activity against L1210 cells (48h) Growth inhibitory activity against L1210 cells (48h)	[PMID: 9154970]
L1210	IC₅₀	> 100 μM Compound: 7	Growth inhibitory activity against L1210 cells (96h) Growth inhibitory activity against L1210 cells (96h)	[PMID: 9154970]
L1210	IC₅₀	> 100 μM Compound: Spd, spermidine	Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay	[PMID: 18558490]
RAW264.7	CC₅₀	> 100 μM Compound: Spermidine	Cytotoxicity against mouse RAW264.7 cells assessed as cell growth inhibition after 72 hrs by Alamar blue dye-based assay Cytotoxicity against mouse RAW264.7 cells assessed as cell growth inhibition after 72 hrs by Alamar blue dye-based assay	[PMID: 27793448]

In Vitro

Spermidine?plays a crucial role of against abiotic stresses, such as salt, drought, heat, and salinity-alkalinity stresses in tomato, cucumber, rice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

145.25

Formula

C₇H₁₉N₃

CAS No.

124-20-9

Appearance

<23°C Solid,>25°C Liquid

Color

Colorless to light yellow

SMILES

NCCCCNCCCN

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (688.47 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (114.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.8847 mL	34.4234 mL	68.8468 mL
5 mM	1.3769 mL	6.8847 mL	13.7694 mL
10 mM	0.6885 mL	3.4423 mL	6.8847 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (11.50 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (11.50 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (11.50 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (344.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.0%

Select Batch:

Data Sheet (268 KB) SDS (477 KB)

COA (239 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang L, et al. The Alleviation of Heat Damage to Photosystem II and Enzymatic Antioxidants by Exogenous Spermidine in Tall Fescue. Front Plant Sci. 2017 Oct 12;8:1747. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	6.8847 mL	34.4234 mL	68.8468 mL	172.1170 mL
	5 mM	1.3769 mL	6.8847 mL	13.7694 mL	34.4234 mL
	10 mM	0.6885 mL	3.4423 mL	6.8847 mL	17.2117 mL
	15 mM	0.4590 mL	2.2949 mL	4.5898 mL	11.4745 mL
	20 mM	0.3442 mL	1.7212 mL	3.4423 mL	8.6059 mL
	25 mM	0.2754 mL	1.3769 mL	2.7539 mL	6.8847 mL
	30 mM	0.2295 mL	1.1474 mL	2.2949 mL	5.7372 mL
	40 mM	0.1721 mL	0.8606 mL	1.7212 mL	4.3029 mL
	50 mM	0.1377 mL	0.6885 mL	1.3769 mL	3.4423 mL
	60 mM	0.1147 mL	0.5737 mL	1.1474 mL	2.8686 mL
	80 mM	0.0861 mL	0.4303 mL	0.8606 mL	2.1515 mL
	100 mM	0.0688 mL	0.3442 mL	0.6885 mL	1.7212 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Spermidine Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spermidine124-20-9Endogenous MetabolitecellmembranestabilityphotosystemPSIIH₂O₂Inhibitorinhibitorinhibit

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Spermidine

Customer Review

Other Forms of Spermidine:

Spermidine Related Antibodies

19 Publications Citing Use of MCE Spermidine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Spermidine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Spermidine Related Classifications

Keywords:

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