Cat. No.: HY-B1777

Spermine

(Synonyms: NSC 268508; Neuridine)

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Spermine is a natural antioxidant and anti-inflammatory agent. Spermine is known to inhibit some bacterial cultures, especially strains of Staphylococcus aureus. Spermine can reversibly inhibits DNA synthetic response, mixed lymphocyte response and the induction of cytolytic lymphocyte response in primary cultures of murine spleen cells. Spermine is a polyamine nitric oxide donor that can provide nitric oxide to platelets and inhibit platelet activation to a certain extent concentration-dependently. Spermine occurs in mammalian tissues, plants, bacteria, ribosomes and bacteriophage. Spermine inhibits primary human embryo lung fibroblasts in vitro.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 71-44-3
Formula: C₁₀H₂₆N₄
Molecular Weight:202.34

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Spermine Chemical Structure

CAS No.: 71-44-3

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	>100 μM Compound: Spm, spermine	Cytotoxicity against CHO cells after 48 hrs by MTT assay Cytotoxicity against CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO	IC₅₀	>100 μM Compound: Spm, spermine	Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO-MG	IC₅₀	>100 μM Compound: 3, spermine	Inhibition of cell growth in CHO-MG cells in the presence of DFMO Inhibition of cell growth in CHO-MG cells in the presence of DFMO	[PMID: 16392808]
Fibroblast	IC₅₀	>500 μM Compound: Spermine	Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	>500 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	14.6 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	14.9 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	356.7 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	422.1 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
HeLa	IC₅₀	50 μM Compound: spermine	Ability to inhibit 50% growth of HeLa cells Ability to inhibit 50% growth of HeLa cells	[PMID: 1875339]
HeLa	IC₅₀	70 μM Compound: spermine	Ability to cause aggregation of purified HeLa cell DNA Ability to cause aggregation of purified HeLa cell DNA	[PMID: 1875339]
Huh-7	CC₅₀	>50 μM Compound: Spermine	Cytotoxicity against human HuH7 cells Cytotoxicity against human HuH7 cells	[PMID: 24177358]
Huh-7	EC₅₀	>50 μM Compound: Spermine	Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy	[PMID: 24177358]
L1210	IC₅₀	0.39 μM Compound: Spermine	Compound was tested for inhibitory concentration required to inhibit 50% of [14C]- putrescine uptake. Compound was tested for inhibitory concentration required to inhibit 50% of [14C]- putrescine uptake.	[PMID: 9871574]
L1210	IC₅₀	54.5 μM Compound: Spm, spermine	Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay	[PMID: 18558490]

In Vitro

Spermine (40-100 μg/mL, 5 d) has ID₅₀ of 25 and 35 in H.E.L. cells and H.Ep.2 cells, respectively^[2].
Spermine (10 μg/well, when added up to 29 h after inception of culture) can inhibit the bacterial Lipopolysaccharide (HY-D1056) in BALB/c spleen cells^[3].
Spermine (5-10 μg/well, when added within 22 h after inception of culture) can completely inhibit concanavalin A (HY-P2149) in BALB/c spleen cells^[3].
Spermine (0.5 mM) shows 50% inhibition of peroxidation reaction in brain phospholipid liposomes^[4].
Spermine tetrahydrochloride (10-50 mM) increases the concentration of NO in platelets and inhibits the expression of CD62p concentration-dependently^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Spermine (50-250 nM, intrastriatally) produces severe cell loss and degeneration as well as neuronal damage around the site of injection^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 71-44-3
Appearance <28°C Solid,>30°C Liquid
Molecular Weight 202.34
Formula C₁₀H₂₆N₄
Color Colorless to off-white
SMILES

NCCCNCCCCNCCCN
Synonyms

NSC 268508; Neuridine
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (9)

All In Vivo Efficacy StudyBio/Physico-chemical AssayCell Imaging/StainingWB

Sort by:

Journal Impact Factor

Cell

Tumor-produced ammonia is metabolized by regulatory T cells to further impede anti-tumor immunity. [Abstract]

2026 Jan 22;189(2):418-434.e24. PMID: 41448182
Nat Commun

Metabolic support protects mucosa from ferroptosis in radiation-induced mucositis. [Abstract]

2025 Dec 8. PMID: 41361241

Spermine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 8. [Abstract]

Immunofluorescence showed the expression of JunD in basal cells before and within 9 days after irradiation (Spermine, 10 mg/kg, ip, 16 days).
Basic Res Cardiol

Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia-reperfusion injury. [Abstract]

2021 Dec 16;116(1):65. PMID: 34914018

Spermine purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]

Spermine (5 mg/kg, ip, single dose) treatment failed to alleviate no-reflow phenomenon and enhance microvascular perfusion.

Spermine purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]

The beneficial effects of HINT2 on nitrite levels, eNOS expression, and eNOS phosphorylation were completely suppressed after Spermine (10 μM, 2 h) treatment.
Acta Physiol

The CaSR/TRPV4 coupling mediates pro-inflammatory macrophage function. [Abstract]

2023 Apr;237(4):e13926. PMID: 36606511
J Biol Chem

Different charged biopolymers induce α-synuclein to form fibrils with distinct structures. [Abstract]

2024 Nov;300(11):107862. PMID: 39374778

Spermine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]

ThT kinetics of α-synuclein aggregation in the presence of different concentrations of Spermine (0-10 mM).

Spermine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]

Statistical analysis was performed on the maximum fluorescence intensity (Fmax) of the kinetic curve after 99 hours of Spermine (3.2-16 μM) incubation.
PLoS Genet

A C. elegans model of copper deficiency: Dietary interventions rescue CTR1/CHCA-1 copper transporter mutant phenotype. [Abstract]

2026 Jan 23;22(1):e1012013. PMID: 41575937
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Virus Res

PEDV infection affects the expression of polyamine-related genes inhibiting viral proliferation. [Abstract]

2022 Apr 15:312:198708. PMID: 35151773
Res Sq

2026 May 18.

Solvent & Solubility

In Vitro:

H₂O : ≥ 200 mg/mL (988.44 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.9422 mL

24.7109 mL

49.4218 mL

5 mM

0.9884 mL

4.9422 mL

9.8844 mL

10 mM

0.4942 mL

2.4711 mL

4.9422 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (477 KB)
- English - EN (477 KB)
- Français - FR (477 KB)
- Deutsch - DE (477 KB)
- Norwegian - NO (477 KB)
- Español - ES (477 KB)
- Swedish - SV (477 KB)
- Italian - IT (477 KB)
- Korean - KR (477 KB)
- Portuguese - PT (477 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. U. Bachrach & B. Reches, (1966) Enzymic assay for spermine and spermidine, Analytical Biochemistry, Volume 17, Issue 1, Volume 17, Issue 1, ISSN 0003-2697.

[2]. Dawson, M., & Dryden, W. F. (1969). The toxicity of spermine and spermidine to cells in culture. Biochemical pharmacology, 18(6), 1307–1313. [Content Brief]

[3]. Byrd, W. J., et al., (1977). Synthetic polyamines added to cultures containing bovine sera reversibly inhibit in vitro parameters of immunity. Nature, 267(5612), 621–623. [Content Brief]

[4]. Løvaas, E., & Carlin, G. (1991). Spermine: an anti-oxidant and anti-inflammatory agent. Free radical biology & medicine, 11(5), 455–461. [Content Brief]

[5]. WANG Jing, et al., (2022) Preliminary Study on Spermine Tetrahydrochloride Inhibition of Platelet Activation[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(3): 315-319.

[6]. Otsuki, M., et al., (1995). In vivo pharmacological study of spermine-induced neurotoxicity. Neuroscience letters, 196(1-2), 81–84. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	4.9422 mL	24.7109 mL	49.4218 mL	123.5544 mL
	5 mM	0.9884 mL	4.9422 mL	9.8844 mL	24.7109 mL
	10 mM	0.4942 mL	2.4711 mL	4.9422 mL	12.3554 mL
	15 mM	0.3295 mL	1.6474 mL	3.2948 mL	8.2370 mL
	20 mM	0.2471 mL	1.2355 mL	2.4711 mL	6.1777 mL
	25 mM	0.1977 mL	0.9884 mL	1.9769 mL	4.9422 mL
	30 mM	0.1647 mL	0.8237 mL	1.6474 mL	4.1185 mL
	40 mM	0.1236 mL	0.6178 mL	1.2355 mL	3.0889 mL
	50 mM	0.0988 mL	0.4942 mL	0.9884 mL	2.4711 mL
	60 mM	0.0824 mL	0.4118 mL	0.8237 mL	2.0592 mL
	80 mM	0.0618 mL	0.3089 mL	0.6178 mL	1.5444 mL
	100 mM	0.0494 mL	0.2471 mL	0.4942 mL	1.2355 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Spermine

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Clinical Compound Library

Human Endogenous Metabolite Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Antibacterial Compound Library

Medicine Food Homology Compound Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Pediatric Drug Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Coagulation and Anti-coagulation Compound Library

Animal-Sourced Natural Product Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Clinical Compound Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

Flavor Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Mouse Metabolite Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Amino Acid Metabolite Compound Library

Mongolian Medicine Compound Library

Chinese Baijiu Compound Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Spermine

All Endogenous Metabolite Isoforms

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Publications (9)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Spermine Related Classifications

Cat. No.: HY-B1777