Cat. No.: HY-B1777A

Spermine tetrahydrochloride

10 Cited Publications

Customer Review

Based on 10 publication(s) in Google Scholar

Spermine tetrahydrochloride is a polyamine nitric oxide donor that can provide nitric oxide to platelets and inhibit platelet activation to a certain extent concentration-dependently. Spermine tetrahydrochloride occurs in mammalian tissues, plants, bacteria, ribosomes and bacteriophage. Spermine tetrahydrochloride inhibits primary human embryo lung fibroblasts in vitro. Spermine is a natural antioxidant and anti-inflammatory agent. Spermine is known to inhibit some bacterial cultures, especially strains of Staphylococcus aureus. Spermine induces neurotoxicity in the striarum dose-dependently. Spermine can reversibly inhibits DNA synthetic response, mixed lymphocyte response and the induction of cytolytic lymphocyte response in primary cultures of murine spleen cells.

For research use only. We do not sell to patients.

Purity: 99.66%
CAS No.: 306-67-2
Formula: C₁₀H₃₀Cl₄N₄
Molecular Weight:348.18

Storage:

RT, protect from light.

In solvent -80°C, 1 year , -20°C, 6 months

Spermine tetrahydrochloride Chemical Structure

CAS No.: 306-67-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (10)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All DNA/RNA Synthesis Isoforms :

In Vitro

Spermine tetrahydrochloride (10-50 mM) increases the concentration of NO in platelets and inhibits the expression of CD62p concentration-dependently^[2].
Spermine (10 μg/well, when added up to 29 h after inception of culture) can inhibit the bacterial Lipopolysaccharide (HY-D1056) in BALB/c spleen cells^[3].
Spermine (5-10 μg/well, when added within 22 h after inception of culture) can completely inhibit concanavalin A (HY-P2149) in BALB/c spleen cells^[3].
Spermine (40-100 μg/mL, 5 d) has ID₅₀ of 25 μg/mL and 35 μg/mL in H.E.L. cells and H.Ep.2 cells, respectively^[4].
Spermine (0.5 mM) shows 50% inhibition of peroxidation reaction in brain phospholipid liposomes^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Spermine (50-250 nM, intrastriatally) produces severe cell loss and degeneration as well as neuronal damage around the site of injection^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 306-67-2
Appearance Solid
Molecular Weight 348.18
Formula C₁₀H₃₀Cl₄N₄
Color White to off-white
SMILES

NCCCNCCCCNCCCN.Cl.Cl.Cl.Cl
Structure Classification
- Others
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

In solvent	-80°C	1 year
	-20°C	6 months

Publications (10)

All In Vivo Efficacy StudyBio/Physico-chemical AssayCell Imaging/StainingWB

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Journal Impact Factor

Cell

Tumor-produced ammonia is metabolized by regulatory T cells to further impede anti-tumor immunity. [Abstract]

2026 Jan 22;189(2):418-434.e24. PMID: 41448182
Nat Commun

Metabolic support protects mucosa from ferroptosis in radiation-induced mucositis. [Abstract]

2025 Dec 8. PMID: 41361241

Spermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 8. [Abstract]

Immunofluorescence showed the expression of JunD in basal cells before and within 9 days after irradiation (Spermine, 10 mg/kg, ip, 16 days).
Basic Res Cardiol

Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia-reperfusion injury. [Abstract]

2021 Dec 16;116(1):65. PMID: 34914018

Spermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]

Spermine (5 mg/kg, ip, single dose) treatment failed to alleviate no-reflow phenomenon and enhance microvascular perfusion.

Spermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]

The beneficial effects of HINT2 on nitrite levels, eNOS expression, and eNOS phosphorylation were completely suppressed after Spermine (10 μM, 2 h) treatment.
Chin Med

Heterophyllin B ameliorates diabetic lower limb ischemia by inhibiting SMOX to activate the Nrf2 antioxidant pathway. [Abstract]

2026 Jun 10;21(1):167. PMID: 42271493
Acta Physiol

The CaSR/TRPV4 coupling mediates pro-inflammatory macrophage function. [Abstract]

2023 Apr;237(4):e13926. PMID: 36606511
J Biol Chem

Different charged biopolymers induce α-synuclein to form fibrils with distinct structures. [Abstract]

2024 Nov;300(11):107862. PMID: 39374778

Spermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]

ThT kinetics of α-synuclein aggregation in the presence of different concentrations of Spermine (0-10 mM).

Spermine tetrahydrochloride purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]

Statistical analysis was performed on the maximum fluorescence intensity (Fmax) of the kinetic curve after 99 hours of Spermine (3.2-16 μM) incubation.
PLoS Genet

A C. elegans model of copper deficiency: Dietary interventions rescue CTR1/CHCA-1 copper transporter mutant phenotype. [Abstract]

2026 Jan 23;22(1):e1012013. PMID: 41575937
Virus Res

PEDV infection affects the expression of polyamine-related genes inhibiting viral proliferation. [Abstract]

2022 Apr 15:312:198708. PMID: 35151773
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Res Sq

2026 May 18.

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (143.60 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8721 mL

14.3604 mL

28.7208 mL

5 mM

0.5744 mL

2.8721 mL

5.7442 mL

10 mM

0.2872 mL

1.4360 mL

2.8721 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. U. Bachrach & B. Reches, (1966) Enzymic assay for spermine and spermidine, Analytical Biochemistry, Volume 17, Issue 1, Volume 17, Issue 1, ISSN 0003-2697.

[2]. WANG Jing, et al., (2022) Preliminary Study on Spermine Tetrahydrochloride Inhibition of Platelet Activation[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(3): 315-319.

[3]. Byrd, W. J., et al., (1977). Synthetic polyamines added to cultures containing bovine sera reversibly inhibit in vitro parameters of immunity. Nature, 267(5612), 621–623. [Content Brief]

[4]. Dawson, M., & Dryden, W. F. (1969). The toxicity of spermine and spermidine to cells in culture. Biochemical pharmacology, 18(6), 1307–1313. [Content Brief]

[5]. Løvaas, E., & Carlin, G. (1991). Spermine: an anti-oxidant and anti-inflammatory agent. Free radical biology & medicine, 11(5), 455–461. [Content Brief]

[6]. Otsuki, M., et al., (1995). In vivo pharmacological study of spermine-induced neurotoxicity. Neuroscience letters, 196(1-2), 81–84. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.8721 mL	14.3604 mL	28.7208 mL	71.8019 mL
	5 mM	0.5744 mL	2.8721 mL	5.7442 mL	14.3604 mL
	10 mM	0.2872 mL	1.4360 mL	2.8721 mL	7.1802 mL
	15 mM	0.1915 mL	0.9574 mL	1.9147 mL	4.7868 mL
	20 mM	0.1436 mL	0.7180 mL	1.4360 mL	3.5901 mL
	25 mM	0.1149 mL	0.5744 mL	1.1488 mL	2.8721 mL
	30 mM	0.0957 mL	0.4787 mL	0.9574 mL	2.3934 mL
	40 mM	0.0718 mL	0.3590 mL	0.7180 mL	1.7950 mL
	50 mM	0.0574 mL	0.2872 mL	0.5744 mL	1.4360 mL
	60 mM	0.0479 mL	0.2393 mL	0.4787 mL	1.1967 mL
	80 mM	0.0359 mL	0.1795 mL	0.3590 mL	0.8975 mL
	100 mM	0.0287 mL	0.1436 mL	0.2872 mL	0.7180 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Spermine tetrahydrochloride

10 Cited Publications

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Spermine tetrahydrochloride

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Publications Citing Use of MedChemExpress (MCE) Spermine tetrahydrochloride

All Endogenous Metabolite Isoforms

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Spermine tetrahydrochloride Related Classifications

HY-B1777A Related Classifications