Ampicillin (Synonyms: D-(-)-α-Aminobenzylpenicillin)

Name: Ampicillin
Brand: MedChemExpress
SKU: HY-B0522
Rating: 5.0 (80 reviews)

Cat. No.: HY-B0522 Purity: 99.92%: Data Sheet
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Ampicillin is a broad-spectrum beta-lactam antibiotic against a variety of gram-positive and gram-negative bacteria.

For research use only. We do not sell to patients.

CAS No. : 69-53-4

Size	Stock	Quantity
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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Based on 80 publication(s) in Google Scholar

Other Forms of Ampicillin:

80 Publications Citing Use of MCE Ampicillin

Cell Proliferation/Viability Assay

PK/PD Analysis

Bio/Physico-chemical Assay

Microbiological Assay

: Ampicillin purchased from MedChemExpress. Usage Cited in: J Exp Med. 2026 Mar 2;223(3):e20241287. [Abstract]; Optical density at 600 nm was measured for each culture and normalized to the media (e.g. Ampicillin).

: Ampicillin purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141. [Abstract]; Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. TET, Tetracycline. DOX, Doxycycline. E, Erythromycin. AZI, Azithromycin. P, Penicillin. CAZ, Ceftazidime. AMP, Ampicillin. CEZ, Cefazolin. CEF, Cefotaxime. OXA, Oxacillin. CRO, Ceftriaxone. AMK, Amikacin. GEN, Gentamycin, KANA, Kanamycin. TOB, Tobramycin. SPC, Spectinomycin.

: Ampicillin purchased from MedChemExpress. Usage Cited in: Microorganisms. 2024 Mar 13;12(3):575.; Percentages of E. coli strains showing antimicrobial resistance to different antimicrobial agents. AMP, Ampicillin; CAZ, Ceftazidime; IPM, Imipenem; MRP, Meropenem; GEN, Gentamicin; OFX, Ofloxacin; SXT, Trimethoprim–sulfamethoxazole; TET, Tetracycline; TGC, Tigecycline; CL, Colistin; FFC, Florfenicol.

: Ampicillin purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2282790. [Abstract]; Depletion of CD8+ T cells in ABX-treated B16-F0 tumor-bearing mice dampened the antitumor effect of butyrate combined with anti-PD-1 (Figure 4b–e). Decreased serum IFN-γ and TNF-α production during the depletion of CD8+ T cells was also observed. ABX：an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole, 1 g/L Neomycin and 1 g/L Ampicillin.

: Ampicillin purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2249143. [Abstract]; Targeted metabolomics analysis of gut microbial metabolites (AA, PA, and BA) in colon contents (feces) and serum from mice with or without ABX treatment (n = 6 per group). ABX: an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole,1 g/L Neomycin and 1 g/L Ampicillin

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Protocol
Purity & Documentation
References
Customer Review

Description

Ampicillin is a broad-spectrum beta-lactam antibiotic against a variety of gram-positive and gram-negative bacteria.

IC₅₀ & Target

β-lactam

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	10000 μM Compound: Ampicillin	TP_TRANSPORTER: inhibition of Gly-Sar uptake (Gly-Sar: 25000 uM) in PEPT1-expressing CHO cells TP_TRANSPORTER: inhibition of Gly-Sar uptake (Gly-Sar: 25000 uM) in PEPT1-expressing CHO cells	[PMID: 8956326]
Erythrocyte	IC₅₀	0.9 μM Compound: Ampicillin	Hemolytic activity against human RBC incubated for 1 hr by microplate reader analysis Hemolytic activity against human RBC incubated for 1 hr by microplate reader analysis	[PMID: 39116273]
HEK293	CC₅₀	> 100 μM Compound: Ampicillin	Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay	[PMID: 30392371]

In Vitro

Ampicillin inhibits the growth of E. coli of swine origin in a dose-dependent manner. The effective inhibitory concentration of Ampicillin was 2.5 uG/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ampicillin is very effective in alleviating the symptoms of hemorrhagic enteritis in a 11-week old pig^[1]. Ampicillin produces maximum concentrations in bile twice as high as in serum. The peak concentration of ampicillin after an oral dose is as twice as high in portal blood as in peripheral blood^[2]. Ampicillin provides neuroprotection against ischemia-reperfusion brain injury. Ampicillin reduces the activities of MMPs and increases the expression level of GLT-1. Pretreatment with ampicillin significantly reduces medial hippocampal cell death following global forebrain ischemia^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

349.40

Formula

C₁₆H₁₉N₃O₄S

CAS No.

69-53-4

Appearance

Solid

Color

White to off-white

SMILES

OC([C@@H]1N(C2=O)[C@]([C@@H]2NC([C@@H](C3=CC=CC=C3)N)=O)([H])SC1(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 4.9 mg/mL (14.02 mM; ultrasonic and warming and adjust pH to 9 with NaOH and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8620 mL	14.3102 mL	28.6205 mL
5 mM	0.5724 mL	2.8620 mL	5.7241 mL
10 mM	0.2862 mL	1.4310 mL	2.8620 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (274 KB) SDS (480 KB)

COA (247 KB) LCMS (86 KB)

Handling Instructions (2659 KB)

References

[1]. Chopra SL, et al. Effect of Ampicillin on E. Coli of Swine Origin. Can J Comp Med Vet Sci. 1963 Sep;27(9):223-7. [Content Brief]

[2]. Lund B, et al. Ampicillin in portal and peripheral blood and bile after oral administration of ampicillin andpivampicillin. Eur J Clin Pharmacol. 1974;7(2):133-5. [Content Brief]

[3]. Lee KE, et al. The neuroprotective mechanism of ampicillin in a mouse model of transient forebrain ischemia. Korean J Physiol Pharmacol. 2016 Mar;20(2):185-92. [Content Brief]

Animal Administration
^[3]

Mice: Ampicillin is dissolved in normal saline. Male C57BL/6 mice were anesthetized with halothane and subjected to bilateral common carotid artery occlusion for 40 min. Before transient forebrain ischemia, ampicillin (200 mg/kg, intraperitoneally [i.p.]) or penicillin G (6,000 U/kg or 20,000 U/kg, i.p.) was administered daily for 5 days. In the control animals, saline was administered at the same volume and time schedule^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Chopra SL, et al. Effect of Ampicillin on E. Coli of Swine Origin. Can J Comp Med Vet Sci. 1963 Sep;27(9):223-7. [Content Brief]

[2]. Lund B, et al. Ampicillin in portal and peripheral blood and bile after oral administration of ampicillin andpivampicillin. Eur J Clin Pharmacol. 1974;7(2):133-5. [Content Brief]

[3]. Lee KE, et al. The neuroprotective mechanism of ampicillin in a mouse model of transient forebrain ischemia. Korean J Physiol Pharmacol. 2016 Mar;20(2):185-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.8620 mL	14.3102 mL	28.6205 mL	71.5512 mL
	5 mM	0.5724 mL	2.8620 mL	5.7241 mL	14.3102 mL
	10 mM	0.2862 mL	1.4310 mL	2.8620 mL	7.1551 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Ampicillin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ampicillin69-53-4D-(-)-α-AminobenzylpenicillinBacterialAntibioticInhibitorinhibitorinhibit

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Ampicillin (Synonyms: D-(-)-α-Aminobenzylpenicillin)

Customer Review

Other Forms of Ampicillin:

Ampicillin Related Antibodies

80 Publications Citing Use of MCE Ampicillin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Ampicillin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ampicillin Related Classifications

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