Atropine methyl bromide (Synonyms: Methylatropine bromide)

Name: Atropine methyl bromide
Brand: MedChemExpress
SKU: HY-112076
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Cat. No.: HY-112076 Pureza: 99.84%: Ficha de datos
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Atropine methyl bromide (Methylatropine bromide), an antagonist of muscarinic acetylcholine receptors (mAChR), is a quaternary ammonium salt of atropine and an active molecule used for pupil dilation during ophthalmic examinations. Due to its high polarity, it is used to relieve pyloric spasm in infants. It is less likely than atropine to cross the central nervous system.

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Atropine methyl bromide Estructura química

No. CAS : 2870-71-5

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	Obtener un presupuesto
100 mg	Obtener un presupuesto

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Based on 1 Customer Validation

Other Forms of Atropine methyl bromide:

Atropine methyl nitrate In-stock
Atropine methyl bromide (Standard) Obtener un presupuesto

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas mAChR:

Ver todas las isoformas

mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

Actividad biológica
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Referencias
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Descripciòn

In Vivo

Atropine methyl bromide (2.5 mg/kg; nasal; 40 pulses over 1 min) provides 100% survival against lethal sarin inhalation exposure in guinea pigs, while normalizing peripheral cholinergic, respiratory, cardiac, and biochemical toxic effects^[1].
Atropine methyl bromide (15-20 mg/kg; i.p.; single injection prior to behavioral run; 50 μg/rat; hippocampal; single injection prior to behavioral run) produces significant impairments in complex operant conditioning task performance in rats when administered directly into the hippocampus at 50 μg/rat (unilateral or bilateral), but only produces a single significant behavioral change at the highest tested intraperitoneal dose of 20 mg/kg^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Hartley (male, adult, 250-300 g, sarin toxicity model)^[1]
Dosage:	2.5 mg/kg
Administration:	nasal; 40 pulses over 1 min
Result:	Achieved 100% survival. Decreased development of muscular tremors, with animals appearing close to normal after 24 hours recovery. Normalized acute decrease in blood oxygen saturation and heart rate. Resulted in body weight loss similar to saline controls, with no significant difference in accessory lung lobe or tracheal wet/dry edema ratios compared to controls. Significantly reduced inhibition of blood acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities compared to untreated sarin-exposed animals. Brought total bronchoalveolar lavage fluid (BALF) protein levels similar to saline controls, and returned surfactant levels to normal. Resulted in BALF alkaline phosphatase levels slightly lower than saline controls. Maintained BALC dead cell counts similar to saline controls, and total BAL cell counts comparable to animals exposed to a lower, non-lethal sarin dose. Significantly decreased blood monocytes, and significantly increased blood eosinophils compared to saline controls. Significantly decreased BALC neutrophils and leukocytes, and increased BALC lymphocytes compared to animals exposed to 677.4 mg/m³ sarin.

Peso molecular

384.31

Fòrmula

C₁₈H₂₆BrNO₃

No. CAS

2870-71-5

Appearance

Solid

Color

White to off-white

SMILES

C[N+]1([C@H]2C[C@H](OC(C(C3=CC=CC=C3)CO)=O)C[C@@H]1CC2)C.[Br-]

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad

In Vitro:

DMSO : 125 mg/mL (325.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6021 mL	13.0103 mL	26.0207 mL
5 mM	0.5204 mL	2.6021 mL	5.2041 mL
10 mM	0.2602 mL	1.3010 mL	2.6021 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.84%

Select Batch:

Ficha de datos (277 KB) SDS (393 KB)

COA (252 KB) HNMR (279 KB) LCMS (94 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Che MM, et al. Post-exposure treatment with nasal atropine methyl bromide protects against microinstillation inhalation exposure to sarin in guinea pigs. Toxicol Appl Pharmacol. 2009;239(3):251-257. [Content Brief]

[2]. Khavari KA, et al. Atropine and atropine methyl bromide effects on behavior of rats. Int J Neuropharmacol. 1967 Jul;6(4):301-6.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6021 mL	13.0103 mL	26.0207 mL	65.0517 mL
	5 mM	0.5204 mL	2.6021 mL	5.2041 mL	13.0103 mL
	10 mM	0.2602 mL	1.3010 mL	2.6021 mL	6.5052 mL
	15 mM	0.1735 mL	0.8674 mL	1.7347 mL	4.3368 mL
	20 mM	0.1301 mL	0.6505 mL	1.3010 mL	3.2526 mL
	25 mM	0.1041 mL	0.5204 mL	1.0408 mL	2.6021 mL
	30 mM	0.0867 mL	0.4337 mL	0.8674 mL	2.1684 mL
	40 mM	0.0651 mL	0.3253 mL	0.6505 mL	1.6263 mL
	50 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3010 mL
	60 mM	0.0434 mL	0.2168 mL	0.4337 mL	1.0842 mL
	80 mM	0.0325 mL	0.1626 mL	0.3253 mL	0.8131 mL
	100 mM	0.0260 mL	0.1301 mL	0.2602 mL	0.6505 mL

Atropine methyl bromide Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Atropine methyl2870-71-5MethylatropinemAChRMuscarinic acetylcholine receptorperipheral anticholinergic agentcholinergic receptor inhibitorsarinplacental barrieracetylcholinesteraseguinea pigsdorsal hippocampusblood-brain barrierbutyrylcholinesteraseratsInhibitorinhibitorinhibit

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Atropine methyl bromide (Synonyms: Methylatropine bromide)

Revisión del cliente

Other Forms of Atropine methyl bromide:

Atropine methyl bromide Related Antibodies

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas mAChR:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Atropine methyl bromide Related Classifications

Keywords:

Productos vistos recientemente:

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SAFETY DATA SHEET (SDS)

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