1. Membrane Transporter/Ion Channel
    Autophagy
  2. TRP Channel
    Autophagy

Capsaicin (Synonyms: (E)-Capsaicin; 8-Methyl-N-vanillyl-trans-6-nonenamide)

Cat. No.: HY-10448 Purity: 98.39%
Handling Instructions

Capsaicin is a TRPV1 agonist with EC50 of 0.29±0.05 μM in HEK293 cells.

For research use only. We do not sell to patients.

Capsaicin Chemical Structure

Capsaicin Chemical Structure

CAS No. : 404-86-4

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg USD 60 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
100 mg USD 96 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

    Capsaicin purchased from MCE. Usage Cited in: Clin Exp Pharmacol Physiol. 2017 Jul;44(7):803-814.

    Western blot analysis showing that the CAP (0.5 mg/kg) injection inhibits the increase of p65 expression and phosphorylation at 24 h after I/R injury. The effects of Capsaicin (CAP) are prevented during the pretreatment of the SST depletor Cysteamine (Cys).
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Capsaicin is a TRPV1 agonist with EC50 of 0.29±0.05 μM in HEK293 cells.

    IC50 & Target

    EC50: 290 nM (hTRPV1, in HEK293 cell)[1]

    In Vitro

    Capsaicin is an agonist of transient receptor potential vanilloid subtype 1 (TRPV1), which is expressed in nociceptive sensory neurons and a range of secretory epithelia, including salivary glands. Capsaicin activates TRPV1, which modulates the permeability of tight junctions (TJ) by regulating the expression and function of putative intercellular adhesion molecules in an ERK-dependent manner[2]. Capsaicin is found to inhibit the growth and proliferation of FaDu cells in a dose- and time-dependent manner. Cells treated with 50, 100, 200, and 300 µM Capsaicin show an augmented decrease in cell growth as the Capsaicin dose increases. In addition, the percentage of viable cells decreases as the incubation time increases. The observed IC50 value is around 150 µM[3].

    In Vivo

    Capsaicin (CAP)-treated animals (Group IV) show increased DNA fragmentation suggesting apoptosis when compare with B(a)P-induced lung cancer-bearing animals (Group II) that show reduced DNA fragmentation. CAP-treated Group IV animals show markedly increased expressions of p53, Bax and caspase-3 with remarkable decrease in the levels of anti-apoptotic protein Bcl-2, when compare with B(a)P-administered lung cancer animals of Group II[4]. Capsaicin causes a dose-dependent reduction of tear secretion in female Wistar/ST rats. Significant effects are observed at doses of 20, 50 and 100 mg/kg. In addition, Capsaicin also causes corneal lesions, and significant effects are observed at doses of 50 and 100 mg/kg[5].

    Clinical Trial
    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 3.2743 mL 16.3714 mL 32.7429 mL
    5 mM 0.6549 mL 3.2743 mL 6.5486 mL
    10 mM 0.3274 mL 1.6371 mL 3.2743 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [3]

    Capsaicin is dissolved in DMSO and stored, and then diluted with appropriate medium before use[3].

    FaDu cells are plated at a density of 1×105 cells/well on 24-well plate. After overnight growth, the cells are treated with various concentrations of Capsaicin (0 μM, 50 μM, 100 μM, 150 μM, 200 μM, 250 μM, 300 μM, and 350 μM) for 24, 48 and 72 hours, with medium replacement every 24 hours. At the end of treatment, 30 µL of the tetrazolium compound MTT, and 270 µL of fresh medium are added. After further incubation for 4 hours at 37°C, 200 µL of 0.1 N HCl in 10% SDS is added into each well to dissolve the tetrazolium crystals. Finally, the absorbance at a wavelength of 540 nm is recorded using an ELISA plate reader[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [4][5]

    Capsaicin is dissolved in olive oil (Mice)[4].
    Capsaicin is dissolved in physiological saline containing 10% ethanol and 10% Tween 80 (Rats)[5].

    Mice[4]
    Healthy male Swiss albino mice weighing 20-25 g (8-10 weeks old) are divided into four groups of six mice each as follows. Group I: control animals receive olive oil throughout the course of the experiment. Group II: animals are administered with Benzo(a)pyrene (B(a)P) (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks. Group III: animals receive Capsaicin alone (10 mg/kg body weight dissolved in olive oil) intraperitoneally once in a week for 14 weeks to assess the cytotoxicity (if any) induced by Capsaicin. Group IV: animals receive B(a)P (as in Group II) along with Capsaicin (10 mg/kg b.wt dissolves in olive oil) intraperitoneally. Capsaicin treatment is started one week prior to the first dose of B(a)P administration and continued for 14 weeks.
    Rats[5]
    Female Wistar/ST rats, 4 d of age, are used. On postnatal day 4, rats are given a single subcutaneous injection of Capsaicin at a dose of 50 mg/kg (or 20 and 100 mg/kg for the dose-response test) dissolved in physiological saline containing 10% ethanol and 10% Tween 80. Vehicle-treated rats receive the vehicle solution alone. The injected rats are housed with their mothers, who are fed standard rat chow and maintained under normal conditions. At 4 weeks of age, the injected rats are separated and housed in cages, and maintained under normal conditions until the initiation of experiments. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    305.41

    Formula

    C₁₈H₂₇NO₃

    CAS No.

    404-86-4

    SMILES

    CC(C)/C=C/CCCCC(NCC1=CC=C(O)C(OC)=C1)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 44 mg/mL

    Capsaicin (CAP) is dissolved in a vehicle containing Tween 80, ethanol and saline, with a proportion of 1:1:8[6].

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    References

    Purity: 98.39%

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Capsaicin
    Cat. No.:
    HY-10448
    Quantity: