1. Membrane Transporter/Ion Channel
    Autophagy
  2. TRP Channel
    Autophagy

Capsaicin (Synonyms: (E)-Capsaicin; 8-Methyl-N-vanillyl-trans-6-nonenamide)

Cat. No.: HY-10448 Purity: 98.39%
Handling Instructions

Capsaicin is a TRPV1 agonist with EC50 of 0.29±0.05 μM in HEK293 cells.

For research use only. We do not sell to patients.
Capsaicin Chemical Structure

Capsaicin Chemical Structure

CAS No. : 404-86-4

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Capsaicin is a TRPV1 agonist with EC50 of 0.29±0.05 μM in HEK293 cells.

IC50 & Target

EC50: 290 nM (hTRPV1, in HEK293 cell)[1]

In Vitro

Capsaicin is an agonist of transient receptor potential vanilloid subtype 1 (TRPV1), which is expressed in nociceptive sensory neurons and a range of secretory epithelia, including salivary glands. Capsaicin activates TRPV1, which modulates the permeability of tight junctions (TJ) by regulating the expression and function of putative intercellular adhesion molecules in an ERK-dependent manner[2]. Capsaicin is found to inhibit the growth and proliferation of FaDu cells in a dose- and time-dependent manner. Cells treated with 50, 100, 200, and 300 µM Capsaicin show an augmented decrease in cell growth as the Capsaicin dose increases. In addition, the percentage of viable cells decreases as the incubation time increases. The observed IC50 value is around 150 µM[3].

In Vivo

Capsaicin (CAP)-treated animals (Group IV) show increased DNA fragmentation suggesting apoptosis when compare with B(a)P-induced lung cancer-bearing animals (Group II) that show reduced DNA fragmentation. CAP-treated Group IV animals show markedly increased expressions of p53, Bax and caspase-3 with remarkable decrease in the levels of anti-apoptotic protein Bcl-2, when compare with B(a)P-administered lung cancer animals of Group II[4]. Capsaicin causes a dose-dependent reduction of tear secretion in female Wistar/ST rats. Significant effects are observed at doses of 20, 50 and 100 mg/kg. In addition, Capsaicin also causes corneal lesions, and significant effects are observed at doses of 50 and 100 mg/kg[5].

Clinical Trial
References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 3.2743 mL 16.3714 mL 32.7429 mL
5 mM 0.6549 mL 3.2743 mL 6.5486 mL
10 mM 0.3274 mL 1.6371 mL 3.2743 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[3]

Capsaicin is dissolved in DMSO and stored, and then diluted with appropriate medium before use[3].

FaDu cells are plated at a density of 1×105 cells/well on 24-well plate. After overnight growth, the cells are treated with various concentrations of Capsaicin (0 μM, 50 μM, 100 μM, 150 μM, 200 μM, 250 μM, 300 μM, and 350 μM) for 24, 48 and 72 hours, with medium replacement every 24 hours. At the end of treatment, 30 µL of the tetrazolium compound MTT, and 270 µL of fresh medium are added. After further incubation for 4 hours at 37°C, 200 µL of 0.1 N HCl in 10% SDS is added into each well to dissolve the tetrazolium crystals. Finally, the absorbance at a wavelength of 540 nm is recorded using an ELISA plate reader[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[4][5]

Capsaicin is dissolved in olive oil (Mice)[4].
Capsaicin is dissolved in physiological saline containing 10% ethanol and 10% Tween 80 (Rats)[5].

Mice[4]
Healthy male Swiss albino mice weighing 20-25 g (8-10 weeks old) are divided into four groups of six mice each as follows. Group I: control animals receive olive oil throughout the course of the experiment. Group II: animals are administered with Benzo(a)pyrene (B(a)P) (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks. Group III: animals receive Capsaicin alone (10 mg/kg body weight dissolved in olive oil) intraperitoneally once in a week for 14 weeks to assess the cytotoxicity (if any) induced by Capsaicin. Group IV: animals receive B(a)P (as in Group II) along with Capsaicin (10 mg/kg b.wt dissolves in olive oil) intraperitoneally. Capsaicin treatment is started one week prior to the first dose of B(a)P administration and continued for 14 weeks.
Rats[5]
Female Wistar/ST rats, 4 d of age, are used. On postnatal day 4, rats are given a single subcutaneous injection of Capsaicin at a dose of 50 mg/kg (or 20 and 100 mg/kg for the dose-response test) dissolved in physiological saline containing 10% ethanol and 10% Tween 80. Vehicle-treated rats receive the vehicle solution alone. The injected rats are housed with their mothers, who are fed standard rat chow and maintained under normal conditions. At 4 weeks of age, the injected rats are separated and housed in cages, and maintained under normal conditions until the initiation of experiments. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

305.41

Formula

C₁₈H₂₇NO₃

CAS No.

404-86-4

SMILES

CC(C)/C=C/CCCCC(NCC1=CC=C(O)C(OC)=C1)=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 44 mg/mL

Capsaicin (CAP) is dissolved in a vehicle containing Tween 80, ethanol and saline, with a proportion of 1:1:8[6].

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

References

Purity: 98.39%

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Capsaicin
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