1. Anti-infection
    Cell Cycle/DNA Damage
    Stem Cell/Wnt
    Autophagy
  2. SARS-CoV
    Casein Kinase
    Autophagy
  3. Emodin

Emodin (Synonyms: Frangula emodin)

Cat. No.: HY-14393 Purity: 99.39%
Handling Instructions

Emodin (Frangula emodin), an anthraquinone derivative isolated from genus Rheum and Polygonum, is an anti-SARS-CoV compound. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 (ACE2) interaction. Emodin inhibits casein kinase-2 (CK2). Anti-inflammatory and anticancer effects. Emodin is a potent selective 11β-HSD1 inhibitor with the IC50 of 186 and 86 nM for human and mouse 11β-HSD1, respectively. Emodin ameliorates metabolic disorder in diet-induced obese mice.

For research use only. We do not sell to patients.

Emodin Chemical Structure

Emodin Chemical Structure

CAS No. : 518-82-1

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Customer Review

Based on 9 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Emodin purchased from MCE. Usage Cited in: Am J Transl Res. 2020 May 15;12(5):1851-1861.

    Emodin resensitizes SW480/5-Fu cells to 5-Fu. SW480/5-Fu cells are treated with 5-Fu or/and Emodin for 72 h. The proliferation of SW480/5-Fu cell is detected by Ki67 immunofluorescence.

    Emodin purchased from MCE. Usage Cited in: Am J Transl Res. 2020 May 15;12(5):1851-1861.

    Emodin reverses 5-Fu chemoresistance in CRC via downregulation of PI3K/Akt signaling pathway. SW480/5-Fu cells are treated with 5-Fu or/and Emodin for 72 h. Then, the protein expressions of Bax, Bcl-2, cleaved caspase3, ERK, Akt, p-ERK and p-Akt in SW480/5-Fu cells are investigated by Western-blot.

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    Description

    Emodin (Frangula emodin), an anthraquinone derivative isolated from genus Rheum and Polygonum, is an anti-SARS-CoV compound. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 (ACE2) interaction[1]. Emodin inhibits casein kinase-2 (CK2). Anti-inflammatory and anticancer effects[2]. Emodin is a potent selective 11β-HSD1 inhibitor with the IC50 of 186 and 86 nM for human and mouse 11β-HSD1, respectively. Emodin ameliorates metabolic disorder in diet-induced obese mice[3].

    IC50 & Target[1][2][3]

    SARS-CoV

     

    CK2α Wild-type

    1.4 μM (IC50, at ATP concentration is 10 μM)

    CK2α Wild-type

    5.9 μM (IC50, at ATP concentration is 50 μM)

    mouse 11β-HSD1

    86 nM (IC50)

    human 11β-HSD1

    186 nM (IC50)

    In Vitro

    Emodin (10-400 μM) blocks the binding of S protein to ACE2 in a dose-dependent manner with the IC50 value of 200 μM[1].
    Emodin (5-50 μM) inhibits the S protein-pseudotyped retrovirus infectivity in a dose-dependent manner. Emodin blocks the SARS-CoV S protein binding to Vero E6 cells[1].
    Emodin inhibits casein kinase-2 (CK2) with IC50s of 5.9, 30.0, and 7.1 μM for CK2α Wild-type, Ile174Ala mutant, and His160Ala mutant at ATP concentration is 50 μM, respectively. The IC50s are 1.40 and 38.00 μM for CK2α Wild-type, and Val66Ala mutant at ATP concentration is 10 μM[2].
    Emodin exhibits low inhibitory activity against mouse and human 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), with an IC50 higher than 1 mM, indicating that Emodin is more than 5000-fold selective for the human and mouse 11β-HSD1 enzymes over the type 2 isoenzyme[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: Vero E6 cells transfected with the plasmid encoding ACE2
    Concentration: 0, 5, 25, 50 μM
    Incubation Time: 24 hours
    Result: Vero cells treated with 50 μM remained 82.4±3.8% viability, the anti-SARS-CoV activity was not due to toxicity.
    In Vivo

    Emodin (single oral administration of 100 or 200 mg/kg) inhibits 11β-HSD1 activity in normal C57BL/6J male mice[3].
    Emodin (100 mg/kg; oral administration; b.i.d.) improves insulin sensitivity and lipid metabolism, and lowers blood glucose and hepatic PEPCK, and glucose-6-phosphatase mRNA in diet-induced obese (DIO) mice[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6J male mice[3]
    Dosage: 100 or 200 mg/kg
    Administration: Acute administered p.o. ; Two hours later, the mice were killed by cervical dislocation,
    Result: Significantly inhibited liver 11β-HSD1 enzymatic activity by 17.6 and 31.3% and mesenteric fat 11β-HSD1 enzymatic activity by 21.5 and 46.7% at 100 or 200 mg/kg, respectively.
    Animal Model: DIO mice (C57BL/6J male mice were fed a formulated research diet)[3]
    Dosage: 100 mg/kg
    Administration: Oral gavage; twice per day; for 35 days
    Result: Reduced fasting glucose concentrations to 77.2% of the vehicle control mice after 7 days of treatment, and these remained significantly lower throughout the treatment period.
    Exhibited a significant reduction in blood glucose levels at all time-points following oral glucose challenge after 24 days of treatment.
    Evoked a significantly greater reduction in blood glucose values 40 and 90 min after insulin injection after 28 days of treatment.
    The serum insulin level was also significantly reduced, to 66.2% of control mice, after 35 days of treatment.
    Improved the lipid profiles. The serum triglyceride and total cholesterol levels were significantly reduced by 19.3 and 12.5% after 35 days of treatment, respectively.
    Caused a 22.7% reduction of non-esterified free fatty acid (NEFA) level.
    Lowered body weight and appetite from day 18 of the treatment; their body weights were reduced by 13.9% at the end of treatment.
    Clinical Trial
    Molecular Weight

    270.24

    Formula

    C₁₅H₁₀O₅

    CAS No.

    518-82-1

    SMILES

    O=C1C2=C(C=C(C)C=C2O)C(C3=CC(O)=CC(O)=C31)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    Acetone : 10.87 mg/mL (40.22 mM; Need ultrasonic)

    DMSO : 5.41 mg/mL (20.02 mM; Need ultrasonic)

    Ethanol : < 1 mg/mL (ultrasonic) (insoluble)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7004 mL 18.5021 mL 37.0041 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL
    10 mM 0.3700 mL 1.8502 mL 3.7004 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  0.5% MC  0.5% Tween-80

      Solubility: 10 mg/mL (37.00 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 1.25 mg/mL (4.63 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 1.25 mg/mL (4.63 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.39%

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    Keywords:

    EmodinFrangula emodinSARS-CoVCasein KinaseAutophagySARS coronavirusmiceinsulinresistancemetabolicsyndromediabeteSARScoronavirusSpikeproteincaseinkinaseCK211β-HSD1Inhibitorinhibitorinhibit

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