2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Ergosterol

Ergosterol is the primary sterol found in fungi, with antioxidative, anti-proliferative, and anti-inflammatory effects.

For research use only. We do not sell to patients.

CAS No. : 57-87-4

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Ergosterol:

Ergosterol Related Antibodies

Top Publications Citing Use of Products
Bio/Physico-chemical Assay
Cell Proliferation/Viability Assay

    Ergosterol purchased from MedChemExpress. Usage Cited in: Nature. 2024 Feb;626(7998):411-418.  [Abstract]

    Representative autoxidation of STY-BODIPY (1 μM) and EggPC liposome containing 5% 7-DHC, 5% Ergosterol, 5% Stigmasterol and 5% Cholesterol respectively.

    Ergosterol purchased from MedChemExpress. Usage Cited in: Nature. 2024 Feb;626(7998):411-418.  [Abstract]

    Cell viability of HEK293T cells treated with RSL3 for 6-8 h after pretreatment of 7-DHC (25 μM), Ergosterol (25 μM), Stigmasterol (25 μM) or Cholesterol (25 μM) for 24 h.

    Ergosterol purchased from MedChemExpress. Usage Cited in: Commun Biol. 2023 Jan 3;6(1):1.  [Abstract]

    The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ergosterol is the primary sterol found in fungi, with antioxidative, anti-proliferative, and anti-inflammatory effects.

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    19.2 μM
    Compound: 19
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
    		[PMID: 33691166]
    BV-2 IC50
    30.6 μM
    Compound: 20
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method
    		[PMID: 21028898]
    COLO 205 IC50
    4.9 μM
    Compound: 6
    Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    		[PMID: 35293752]
    COLO 320 IC50
    6.5 μM
    Compound: 6
    Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    		[PMID: 35293752]
    HeLa IC50
    0.91 μg/mL
    Compound: 1
    Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay
    Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay
    		10.1007/s00044-010-9409-z
    L5178Y EC50
    12.6 μM
    Compound: 7
    Cytotoxicity against mouse L5178Y cells by MTT assay
    Cytotoxicity against mouse L5178Y cells by MTT assay
    		[PMID: 27070198]
    MRC5 IC50
    0.5 μM
    Compound: 6
    Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    		[PMID: 35293752]
    NCI-H157 IC50
    23.3 μM
    Compound: 19
    Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
    Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay
    		[PMID: 33691166]
    In Vivo

    Ergosterol (25, 50 mg/kg, p.o.) significantly mitigates the reduced cardiac performance in rats induced by LPS, increases SOD activity and decreases the formation of MDA, CK-MB, and LDH in LPS-induced sepsis rats[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    396.65

    Formula

    C28H44O
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    C[C@H](C(C)C)/C=C/[C@@H](C)[C@H]1CC[C@@]2([H])C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
    Structure Classification
    Initial Source

    fungi
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    Ethanol : 2 mg/mL (5.04 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5211 mL 12.6056 mL 25.2111 mL
    5 mM 0.5042 mL 2.5211 mL 5.0422 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/0.5% Tween-80 in Saline water

      Solubility: 2.5 mg/mL (6.30 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.91%

    SDS (252 KB)

    COA (249 KB) HNMR (269 KB) RP-HPLC (205 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [2]

    Rats[2]
    Experimental myocardial injury in rats is performed by LPS injection (15 mg/kg). Dexmedetomidine (Dex) is used as a positive control. The experimental animals are randomly divided into five groups (n = 10) as follows: Control group, rats receive 2% gum acacia suspension orally at a dose of 2 mL/kg for 5 days, followed by normal saline injected intraperitoneally on day 5; LPS group, rats receive 2% gum acacia suspension at dose of 2 mL/kg for 5 days with LPS simultaneously injected intraperitoneally day 5; LPS+ Dex group, rats are treated with 2 mg/kg Dex suspension followed by LPS injection on day 5; LPS + Ergosterol (25 mg/kg, 50 mg/kg) groups, 25 or 50 mg/kg Ergosterol are given to rats orally for 5 consecutive days, and LPS is injected on day 5. Twelve hours after LPS treatment, blood samples are collected through the retro-orbital plexus. The serum specimens are centrifugated at 4, 000 × g for 15 min and stored at −80°C until needed. Thereafter, rats are anesthetized and sacrificed. Heart tissues are removed and homogenized in ice-cold phosphate buffered saline (50 mM, pH 7.4). Heart tissue homogenates from different groups are centrifuged at 12, 000 × g for 45 min at 4°C and the supernatants retained for further biochemical evaluations[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol 1 mM 2.5211 mL 12.6056 mL 25.2111 mL 63.0279 mL
    5 mM 0.5042 mL 2.5211 mL 5.0422 mL 12.6056 mL
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    Ergosterol Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ergosterol57-87-4Endogenous MetaboliteInhibitorinhibitorinhibit

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