Ergosterol

Name: Ergosterol
Brand: MedChemExpress
SKU: HY-N0181
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0181 Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Ergosterol is the primary sterol found in fungi, with antioxidative, anti-proliferative, and anti-inflammatory effects.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 57-87-4

사이즈	재고	수량
100 mg	해외재고보유	Estimated Time of Arrival: December 31
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	해외재고보유	Estimated Time of Arrival: December 31
10 g	견적 받기
50 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 3 publication(s) in Google Scholar

Other Forms of Ergosterol:

Lumisterol In-stock
Ergosterol-d₃ In-stock
Ergosterol (Standard) In-stock

3 Publications Citing Use of MCE Ergosterol

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

: Ergosterol purchased from MedChemExpress. Usage Cited in: Nature. 2024 Feb;626(7998):411-418. [Abstract]; Representative autoxidation of STY-BODIPY (1 μM) and EggPC liposome containing 5% 7-DHC, 5% Ergosterol, 5% Stigmasterol and 5% Cholesterol respectively.

: Ergosterol purchased from MedChemExpress. Usage Cited in: Nature. 2024 Feb;626(7998):411-418. [Abstract]; Cell viability of HEK293T cells treated with RSL3 for 6-8 h after pretreatment of 7-DHC (25 μM), Ergosterol (25 μM), Stigmasterol (25 μM) or Cholesterol (25 μM) for 24 h.

: Ergosterol purchased from MedChemExpress. Usage Cited in: Commun Biol. 2023 Jan 3;6(1):1. [Abstract]; The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi.

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Ergosterol is the primary sterol found in fungi, with antioxidative, anti-proliferative, and anti-inflammatory effects.

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	19.2 3 Compound: 19	Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay	[PMID: 33691166]
A549	IC₅₀	19.2 3 Compound: 19	Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay	[PMID: 33691166]
BV-2	IC₅₀	30.6 3 Compound: 20	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method	[PMID: 21028898]
BV-2	IC₅₀	30.6 3 Compound: 20	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method	[PMID: 21028898]
A549	IC₅₀	19.2 3 Compound: 19	Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay	[PMID: 33691166]
COLO 205	IC₅₀	4.9 3 Compound: 6	Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
COLO 205	IC₅₀	4.9 3 Compound: 6	Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
BV-2	IC₅₀	30.6 3 Compound: 20	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method	[PMID: 21028898]
COLO 320	IC₅₀	6.5 3 Compound: 6	Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
COLO 320	IC₅₀	6.5 3 Compound: 6	Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
HeLa	IC₅₀	0.91 6 Compound: 1	Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay	10.1007/s00044-010-9409-z
HeLa	IC₅₀	0.91 6 Compound: 1	Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay	10.1007/s00044-010-9409-z
L5178Y	EC₅₀	12.6 3 Compound: 7	Cytotoxicity against mouse L5178Y cells by MTT assay Cytotoxicity against mouse L5178Y cells by MTT assay	[PMID: 27070198]
L5178Y	EC₅₀	12.6 3 Compound: 7	Cytotoxicity against mouse L5178Y cells by MTT assay Cytotoxicity against mouse L5178Y cells by MTT assay	[PMID: 27070198]
COLO 205	IC₅₀	4.9 3 Compound: 6	Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human COLO 205 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
MRC5	IC₅₀	0.5 3 Compound: 6	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
MRC5	IC₅₀	0.5 3 Compound: 6	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
COLO 320	IC₅₀	6.5 3 Compound: 6	Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human COLO 320 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
NCI-H157	IC₅₀	23.3 3 Compound: 19	Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay	[PMID: 33691166]
NCI-H157	IC₅₀	23.3 3 Compound: 19	Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay	[PMID: 33691166]
HeLa	IC₅₀	0.91 6 Compound: 1	Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) expressed in HeLa cells cotreated with compound and 10 ng/ml TNF-alpha for 24 hr by reporter gene assay	10.1007/s00044-010-9409-z
L5178Y	EC₅₀	12.6 3 Compound: 7	Cytotoxicity against mouse L5178Y cells by MTT assay Cytotoxicity against mouse L5178Y cells by MTT assay	[PMID: 27070198]
MRC5	IC₅₀	0.5 3 Compound: 6	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 35293752]
NCI-H157	IC₅₀	23.3 3 Compound: 19	Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay Antiproliferative activity against human NCI-H157 cells assessed as reduction in cell viability measured after 72 hrs by sulforhodamine B assay	[PMID: 33691166]

In Vivo

Ergosterol (25, 50 mg/kg, p.o.) significantly mitigates the reduced cardiac performance in rats induced by LPS, increases SOD activity and decreases the formation of MDA, CK-MB, and LDH in LPS-induced sepsis rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

396.65

화학식

C₂₈H₄₄O

CAS No.

57-87-4

Appearance

Solid

Color

White to yellow

SMILES

C[C@H](C(C)C)/C=C/[C@@H](C)[C@H]1CC[C@@]2([H])C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Structure Classification

Steroids

Initial Source

Microorganisms

Endogenous metabolite

fungi

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

Ethanol : 2 mg/mL (5.04 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5211 mL	12.6056 mL	25.2111 mL
5 mM	0.5042 mL	2.5211 mL	5.0422 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/0.5% Tween-80 in Saline water
Solubility: 2.5 mg/mL (6.30 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.91%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (249 KB) HNMR (269 KB) RP-HPLC (205 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Xu J, et al. Ergosterol Attenuates LPS-Induced Myocardial Injury by Modulating Oxidative Stress and Apoptosis in Rats. Cell Physiol Biochem. 2018;48(2):583-592. [Content Brief]

Animal Administration
^[2]

Rats^[2]
Experimental myocardial injury in rats is performed by LPS injection (15 mg/kg). Dexmedetomidine (Dex) is used as a positive control. The experimental animals are randomly divided into five groups (n = 10) as follows: Control group, rats receive 2% gum acacia suspension orally at a dose of 2 mL/kg for 5 days, followed by normal saline injected intraperitoneally on day 5; LPS group, rats receive 2% gum acacia suspension at dose of 2 mL/kg for 5 days with LPS simultaneously injected intraperitoneally day 5; LPS+ Dex group, rats are treated with 2 mg/kg Dex suspension followed by LPS injection on day 5; LPS + Ergosterol (25 mg/kg, 50 mg/kg) groups, 25 or 50 mg/kg Ergosterol are given to rats orally for 5 consecutive days, and LPS is injected on day 5. Twelve hours after LPS treatment, blood samples are collected through the retro-orbital plexus. The serum specimens are centrifugated at 4, 000 × g for 15 min and stored at −80°C until needed. Thereafter, rats are anesthetized and sacrificed. Heart tissues are removed and homogenized in ice-cold phosphate buffered saline (50 mM, pH 7.4). Heart tissue homogenates from different groups are centrifuged at 12, 000 × g for 45 min at 4°C and the supernatants retained for further biochemical evaluations^[2].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Xu J, et al. Ergosterol Attenuates LPS-Induced Myocardial Injury by Modulating Oxidative Stress and Apoptosis in Rats. Cell Physiol Biochem. 2018;48(2):583-592. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.5211 mL	12.6056 mL	25.2111 mL	63.0279 mL
Ethanol	5 mM	0.5042 mL	2.5211 mL	5.0422 mL	12.6056 mL

Ergosterol Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ergosterol57-87-4Endogenous MetaboliteInhibitorinhibitorinhibit

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Ergosterol

고객리뷰

Other Forms of Ergosterol:

Ergosterol Related Antibodies

3 Publications Citing Use of MCE Ergosterol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Ergosterol Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report