2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Apoptosis
  3. PPAR Apoptosis
  4. GW6471

GW6471 is a selective peroxisome proliferator-activated receptor α (PPARα) antagonist. GW6471 reduces cancer stem cell viability, proliferation, and spheroid formation. GW6471 induces apoptosis and causes metabolic impairment including energy imbalance. GW6471 can be used for the research of paragangliomas and triple-negative breast cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 880635-03-0

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 82 在庫あり
Solution
10 mM * 1 mL in DMSO USD 82 在庫あり
Solid
5 mg $60 在庫あり
10 mg $100 在庫あり
25 mg $230 在庫あり
50 mg $400 在庫あり
100 mg $680 在庫あり
500 mg $1875 在庫あり
1 g $2850 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 70 publication(s) in Google Scholar

GW6471 関連抗体

Top Publications Citing Use of Products

顧客検証

IF
RT-PCR
WB

    GW6471 purchased from MedChemExpress. Usage Cited in: J Dairy Sci. 2023 Apr;106(4):2271-2288.  [Abstract]

    GW6471 significantly reduces the regulatory effects of GINY and DQW on the protein expression levels of PPARα, CPT-1a, ACOX1, HO-1, and FASN in HepG2 cells.

    GW6471 purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2019 Nov 3;2019:7536803.  [Abstract]

    Cells are treated with PE (50 μM), zileuton (100 μM), and GW6471 (10 μM) for 24 h. α-Actinin staining (n = 6) results in H9c2 cells.

    GW6471 purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2019 Nov 3;2019:7536803.  [Abstract]

    The antihypertrophic effects of zileuton ar counteracted by GW6471 as indicated by increased PE-induced hypertrophic marker transcription, cell surface area, and oxidative stress when compared with those in the PE group and PE+ zileuton group.

    PPAR アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    PPARα PPARβ/δ PPARγ PPAR PPARδ

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    GW6471 is a selective peroxisome proliferator-activated receptor α (PPARα) antagonist. GW6471 reduces cancer stem cell viability, proliferation, and spheroid formation. GW6471 induces apoptosis and causes metabolic impairment including energy imbalance. GW6471 can be used for the research of paragangliomas and triple-negative breast cancer[1][2][3].

    IC50 & Target[1]

    PPARα

     

    体外実験

    GW6471 (7-16 μM; 72 h) reduces cell viability in PTJ64i and PTJ86i head and neck paraganglioma cell lines at 16 μM[1].
    GW6471 (7-16 μM; 72 h) impairs clonogenicity in PTJ64i and PTJ86i head and neck paraganglioma cell lines, with 16 μM causing a more drastic reduction in colony-forming capacity than 7 μM[1].
    GW6471 (4-16 μM; 72 h) dose-dependently reduces cell viability in MDA-MB-231-derived breast cancer stem cell mammospheres, with significant effects observed at 4 μM, 8 μM, and 16 μM after 72 hours of treatment[3].
    GW6471 (8 μM; 72 h) induces significant cytotoxicity in MDA-MB-231-derived breast cancer stem cell mammospheres[3].
    GW6471 (8 μM; 72 h) significantly inhibits spheroid growth in MDA-MB-231-derived breast cancer stem cell mammospheres[3].
    GW6471 (8 μM; 72 h) induces G1 phase cell cycle arrest and intrinsic pathway apoptosis in MDA-MB-231-derived breast cancer stem cell mammospheres[3].
    GW6471 (8 μM; 72 h) activates caspase 3/7, confirming apoptotic induction in MDA-MB-231-derived breast cancer stem cell mammospheres[3].
    GW6471 (8 μM; 72 h) activates p-AMPK, reduces lipid droplet and cholesterol levels, and downregulates mevalonate pathway gene expression in MDA-MB-231-derived breast cancer stem cell mammospheres[3].
    GW6471 (8 μM; 72 h) impairs glucose metabolism in MDA-MB-231-derived breast cancer stem cell mammospheres by downregulating key glycolytic proteins, reducing glucose uptake, and decreasing lactate production[3].
    GW6471 (8 μM; 72 h) upregulates CD36 and induces nuclear translocation of PPARγ in MDA-MB-231-derived breast cancer stem cell mammospheres[3].
    GW6471 (8 μM; 72 h) activates GSK3β and reduces cytoplasmic/nuclear β-catenin while increasing membrane-associated β-catenin in MDA-MB-231-derived breast cancer stem cell mammospheres[3].
    GW6471 (8 μM; 72 h) reduces YAP/TAZ activity, downregulates Rho family proteins, and inhibits invasion in MDA-MB-231-derived breast cancer stem cell mammospheres[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    GW6471 関連抗体

    Cell Viability Assay[1]

    Cell Line: PTJ64i, PTJ86i (head and neck paraganglioma cell lines)
    Concentration: 7 μM; 16 μM
    Incubation Time: 72 h
    Result: Reduced cell viability in PTJ64i and PTJ86i head and neck paraganglioma cell lines at 16 μM.
    Impaired clonogenicity in PTJ64i and PTJ86i head and neck paraganglioma cell lines, with 16 μM causing a more drastic reduction in colony-forming capacity than 7 μM

    Cell Viability Assay[3]

    Cell Line: MDA-MB-231-derived breast cancer stem cell mammospheres
    Concentration: 4 μM; 8 μM; 16 μM
    Incubation Time: 72 h
    Result: Reduced cell viability to ~70% of control at 4 μM.
    Reduced cell viability to ~40% of control at 8 μM.
    Reduced cell viability to ~25% of control at 16 μM.

    Cell Cytotoxicity Assay[3]

    Cell Line: MDA-MB-231-derived breast cancer stem cell mammospheres
    Concentration: 8 μM
    Incubation Time: 72 h
    Result: Increased mean fluorescence intensity (cytotoxicity) in a time-dependent manner over 72 hours.
    Showed significantly higher cytotoxicity levels compared to untreated controls at all time points after 0 hours.

    Cell Cycle Analysis[3]

    Cell Line: MDA-MB-231-derived breast cancer stem cell mammospheres
    Concentration: 8 μM
    Incubation Time: 72 h
    Result: Induced G1 phase cell cycle arrest and intrinsic pathway apoptosis.

    Apoptosis Analysis[3]

    Cell Line: MDA-MB-231-derived breast cancer stem cell mammospheres
    Concentration: 8 μM
    Incubation Time: 72 h
    Result: Increased mean fluorescence intensity indicating caspase 3/7 activation over the 72-hour period.
    分子量

    619.67

    分子式

    C35H36F3N3O4
    CAS 番号
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    CCC(NC[C@@H](N/C(C)=C\C(C1=CC=C(C(F)(F)F)C=C1)=O)CC2=CC=C(OCCC3=C(C)OC(C4=CC=CC=C4)=N3)C=C2)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 83.33 mg/mL (134.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6138 mL 8.0688 mL 16.1376 mL
    5 mM 0.3228 mL 1.6138 mL 3.2275 mL
    10 mM 0.1614 mL 0.8069 mL 1.6138 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  15% Cremophor EL    85% Saline

      Solubility: 5.56 mg/mL (8.97 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.64%

    COA (247 KB) LCMS (94 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6138 mL 8.0688 mL 16.1376 mL 40.3441 mL
    5 mM 0.3228 mL 1.6138 mL 3.2275 mL 8.0688 mL
    10 mM 0.1614 mL 0.8069 mL 1.6138 mL 4.0344 mL
    15 mM 0.1076 mL 0.5379 mL 1.0758 mL 2.6896 mL
    20 mM 0.0807 mL 0.4034 mL 0.8069 mL 2.0172 mL
    25 mM 0.0646 mL 0.3228 mL 0.6455 mL 1.6138 mL
    30 mM 0.0538 mL 0.2690 mL 0.5379 mL 1.3448 mL
    40 mM 0.0403 mL 0.2017 mL 0.4034 mL 1.0086 mL
    50 mM 0.0323 mL 0.1614 mL 0.3228 mL 0.8069 mL
    60 mM 0.0269 mL 0.1345 mL 0.2690 mL 0.6724 mL
    80 mM 0.0202 mL 0.1009 mL 0.2017 mL 0.5043 mL
    100 mM 0.0161 mL 0.0807 mL 0.1614 mL 0.4034 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    GW6471 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    GW6471880635-03-0GW 6471GW-6471PPARApoptosisPeroxisome proliferator-activated receptorsPTJ86iMDA-MB-231cardiomyocytemurine embryonic stem cellscancer stem cellhead and neck paraganglioma cellsPTJ64iperoxisome proliferator-activated receptor αtriple-negative breast cancerPPARαInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    GW6471
    製品番号:
    HY-15372
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.