2. Membrane Transporter/Ion Channel Immunology/Inflammation NF-κB Autophagy Anti-infection
  3. Na+/K+ ATPase IRAK NF-κB Autophagy Mitophagy HSV
  4. Ginsenoside Rb1

ギンセノシドRb1  (Synonyms: Ginsenoside Rb1; Gypenoside III)

製品番号: HY-N0039 純度: 99.75%
COA 取扱説明書 Technical Support

Ginsenoside Rb1, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with an IC50 of 6.3±1.0 μM. Ginsenoside also inhibits IRAK-1 activation and phosphorylation of NF-κB p65 .

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CAS 番号 : 41753-43-9

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カスタマーレビュー

Based on 14 publication(s) in Google Scholar

Other Forms of Ginsenoside Rb1:

ギンセノシドRb1 関連抗体

Top Publications Citing Use of Products

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jun 13;23(1):443.  [Abstract]

    Ginsenoside Rb1 (5 mg/kg; via tail vein injection on days 7, 14, and 21 post-tumor implantation) in BALB/c nude mice (PI3K-mutated A549 cells) exhibited significantly decreased tumor weight and reduced tumor growth rate compared to the Control group. A Schematic timeline of the animal experiment; B Images and weights of dissected tumors from subcutaneous xenograft model mice in each group. C Tumor growth curves for mice in each group;

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jun 13;23(1):443.  [Abstract]

    Ginsenoside Rb1 (5 mg/kg; via tail vein injection on days 7, 14, and 21 post-tumor implantation) in BALB/c nude mice (PI3K-mutated A549 cells) reduced infiltration of inflammatory cells, tighter cell arrangement, more normal cell morphology, uniform cell nuclei, and clear nucleoli. H&E staining images of tumors from each group.

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jun 13;23(1):443.  [Abstract]

    Ginsenoside Rb1 (5 mg/kg; via tail vein injection on days 7, 14, and 21 post-tumor implantation) in BALB/c nude mice (PI3K-mutated A549 cells) compared to the Control group, with a more substantial decrease in Ki67 expression in the Rb1@T-exo group.

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):824.  [Abstract]

    EA.hy926 ECs were pretreated with Ginsenoside Rb1 (Rb1; 3, 10, and 30 μM) for 30 min before ox-LDL/HG for 24 h, Ginsenoside Rb1 dose-dependently restored the declined cell viability in ox-LDL/HG-challenged ECs.

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):824.  [Abstract]

    EA.hy926 ECs were pretreated with Ginsenoside Rb1 (Rb1; 3, 10, and 30 μM) for 30 min before ox-LDL/HG for 24 h. Exposure to ox-LDL/HG promoted the generation of MDA, a marker of oxidative stress, while this was concentrationdependently reversed by Rb1 pretreatment.

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):824.  [Abstract]

    EA.hy926 ECs were pretreated with Ginsenoside Rb1 (Rb1; 3, 10, and 30 μM) for 30 min before ox-LDL/HG for 24 h. Representative images of DHE staining.

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):824.  [Abstract]

    EA.hy926 ECs were pretreated with Ginsenoside Rb1 (Rb1; 10 μM) for 30 min before ox-LDL/HG for 24 h. A Cell apoptosis measured by flow cytometer. B Apoptosis ratio.

    Ginsenoside Rb1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):824.  [Abstract]

    EA.hy926 ECs were pretreated with Ginsenoside Rb1 (Rb1; 10 μM) for 30 min before ox-LDL/HG for 24 h. Cell apoptosis measured by TUNEL assay.

    IRAK アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    IRAK1 IRAK3 IRAK4

    NF-κB アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    HSV アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    HSV-1 HSV-2 HSV

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Ginsenoside Rb1, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with an IC50 of 6.3±1.0 μM. Ginsenoside also inhibits IRAK-1 activation and phosphorylation of NF-κB p65 .

    IC50 & Target[1][2][3][6]

    Na+, K+-ATPase

    6.3 μM (IC50)

    IRAK-1

     

    p65

     

    Autophagy

     

    Mitophagy

     

    HSV-1

     

    Cellular Effect
    Cell Line Type Value Description References
    Erythrocyte IC50
    3.18 μM
    Compound: 70
    Inhibition of AAPH-induced hemolysis of human erythrocytes
    Inhibition of AAPH-induced hemolysis of human erythrocytes
    		[PMID: 32006794]
    体外実験

    Rat brain microsomal Na+, K+-ATPase activity is inhibited significantly and rapidly by Ginsenoside Rb1. The IC50 of Ginsenoside Rb1 for Na+,K+-ATPase is 6.3±1.0 μM. The inhibition is enhanced with increasing the concentration of Ginsenoside Rb1 or decreasing that of Na+ and K+. Kinetic analysis reveals that Ginsenoside is an uncompetitive inhibitor with respect to ATP[1]. Ginsenoside Rb1 significantly inhibits the activation of interleukin-1 receptor-associated kinase-1 (IRAK-1), IKK-β, NF-κB, and MAP kinases (ERK, JNK, and p-38); however, interaction between LPS and Toll-like receptor-4, IRAK-4 activation and IRAK-2 activation are unaffected[2]. Ginsenoside Rb1 is an ingredient of a Chinese medicine Panax ginseng. Ginsenoside Rb1 is a major bioactive compound in the regulating pregnane X receptor (PXR)/NF-κB signaling. Ginsenoside Rb1 is the compound with potent anti-inflammatory activity in ginseng saponin extract (GSE). The concentration for Ginsenoside Rb1 (10 μM) is optimized from a preliminary study to ensure sufficient anti-inflammatory activity and without apparent cytotoxicity. Ginsenoside Rb1 significantly reduces TNF-α-induced upregulation of IL-1β and iNOS mRNA levels, and restores the mRNA levels of PXR and CYP3A4 in LS174T cells. TNF-α causes a significant reduction in PXR protein levels and increase in the ratio of phosphorylated to total NF-κB p65, both of which are significantly abrogated by Ginsenoside Rb1[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ギンセノシドRb1 関連抗体
    体内実験

    Ginsenoside Rb1 at the both doses of 30 mg/kg and 60 mg/kg significantly attenuates the histological lung injury. Ginsenoside Rb1 at the dose of 30 mg/kg and 60 mg/kg both significantly attenuates the histological intestine injury[4]. Ginsenoside Rb1 (Rb1), an ingredient of a Chinese medicine Panax ginseng, has beneficial effects on mesentery microvascular hyperpermeability induced by Lipopolysaccharide (LPS) and the underlying mechanisms. In some rats, Ginsenoside Rb1 (5 mg/kg per hour) is administrated through the left jugular vein 30 min after LPS infusion. Ginsenoside Rb1 decreases caveolae number in endothelial cells of microvessels. Ginsenoside Rb1 ameliorates microvascular hyperpermeability after the onset of endotoxemia and improves intestinal edema through inhibiting caveolae formation and junction disruption, which is correlated to suppression of NF-κB and Src activation[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    1109.29

    分子式

    C54H92O23
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]([C@@]12C)(CC[C@@]3([H])C4(C)C)[C@@](C[C@@H](O)[C@]1([H])[C@]([C@@](CC/C=C(C)/C)(C)O[C@@H]([C@@H]([C@@H](O)[C@@H]5O)O)O[C@@H]5CO[C@@H]([C@@H]([C@@H](O)[C@@H]6O)O)O[C@@H]6CO)([H])CC2)([H])[C@]3(CC[C@@H]4O[C@@](O[C@H](CO)[C@@H](O)[C@@H]7O)([H])[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)C
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (90.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 33.33 mg/mL (30.05 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9015 mL 4.5074 mL 9.0148 mL
    5 mM 0.1803 mL 0.9015 mL 1.8030 mL
    10 mM 0.0901 mL 0.4507 mL 0.9015 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

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    濃度 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (2.25 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (2.25 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.75%

    COA (259 KB) HNMR (431 KB) RP-HPLC (264 KB) MS (70 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [3]

    LS174T cells are seeded in cell imaging dish. After overnight incubation, cells are treated with GSE (100 μg/mL), Ginsenoside Rb1 (10 μM), or CK (10 μM) for 3 hours, followed by an additional incubation with or without TNF-α (20 ng/ml) for 6 hours. At the end of the incubation, cells are harvested and fixed with 4% paraformaldehyde solution at 20°C for 20 minutes. After washing in PBS, cells are permeabilized with 0.2% Triton X-100 in PBS at room temperature for 5 minutes. After incubation in blocking buffer containing 0.1% Triton X-100 and 5% bovine serum albumin, cells are incubated with rabbit NF-κB p65 antibody at 4°C overnight and then with Alexa Fluor 488-conjugated anti-rabbit IgG antibody at room temperature for 30 minutes in 1% bovine serum albumin in PBS. Fluorescence photographs are obtained using a Zeiss 710 confocal microscope[3].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [4][5]

    Mice[4]
    Male C57BL/6 mice (9-12 weeks old; 17-22 g) are randomly allocated into eight groups (n=8 in each group): (1) Sham surgical preparation including isolation of the superior mesenteric artery (SMA) without occlusion is performed (Sham); (2) mice are subjected to II/R without treatment (II/R); (3) mice are subjected to II/R with treatment of normal saline 10 minutes before reperfusion (II/R+NS); (4), (5) mice are treated with 30 mg/kg (II/R+Rb1-30) or 60 mg/kg (II/R+Rb1-60) Ginsenoside Rb1, in which surgery is performed as in the II/R group with administration of the Ginsenoside Rb1 intraperitoneally 10 minutes before reperfusion; (6) mice are subjected to Sham surgery and treated with ATRA (ATRA+Sham), which is the inhibitor of Nrf2/ARE signaling pathway; (7) mice are subjected to II/R and treated with ATRA (ATRA+II/R); (8) mice are subjected to II/R and treated with ATRA and 60 mg/kg Ginsenoside Rb1 as group 5 (ATRA+II/R+Rb1-60). During the last two weeks before the operation, the mice in the group 6, 7, 8 receive ATRA i.p. daily at 10 mg/kg and fed on a vitamin A-deficient diet, and the mice in the other groups receive the equivalent volume of corn oil and fed on a control normal diet.
    Rats[5]
    Male Wistar rats weighing 200-250 g are used. The rats are randomly divided into four groups, 26 animals in each. After being anesthetized with urethane (2 g/kg body wt im), the left femoral vein and left jugular vein of the rat are cannulated. In the LPS group, LPS solution in saline is infused (5 mg/kg per hour) for 90 min via the left femoral vein. The vehicle, instead of LPS solution, is administrated in Sham and Ginsenoside Rb1 alone (Rb1, 5 mg/kg) groups. In the Rb1 posttreatment (LPS+Rb1) group, Ginsenoside Rb1 solution is infused continuously through the left jugular vein 30 min after LPS administration at the dose of 5 mg/kg. Ginsenoside Rb1 solution and the same volume of saline are infused in Ginsenoside Rb1 and Sham groups, respectively, without subsequent LPS administration. In a separate set of experiments, the rats are anesthetized with 2% penobarbital sodium (60 mg/kg body wt ip), and saline, LPS, and Ginsenoside Rb1. After recovery from anesthesia, the animals are allowed access to water and rodent chow, and survival rate is recorded over time until 4 days after LPS stimulation.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.9015 mL 4.5074 mL 9.0148 mL 22.5369 mL
    5 mM 0.1803 mL 0.9015 mL 1.8030 mL 4.5074 mL
    10 mM 0.0901 mL 0.4507 mL 0.9015 mL 2.2537 mL
    15 mM 0.0601 mL 0.3005 mL 0.6010 mL 1.5025 mL
    20 mM 0.0451 mL 0.2254 mL 0.4507 mL 1.1268 mL
    25 mM 0.0361 mL 0.1803 mL 0.3606 mL 0.9015 mL
    30 mM 0.0300 mL 0.1502 mL 0.3005 mL 0.7512 mL
    DMSO 40 mM 0.0225 mL 0.1127 mL 0.2254 mL 0.5634 mL
    50 mM 0.0180 mL 0.0901 mL 0.1803 mL 0.4507 mL
    60 mM 0.0150 mL 0.0751 mL 0.1502 mL 0.3756 mL
    80 mM 0.0113 mL 0.0563 mL 0.1127 mL 0.2817 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Ginsenoside Rb1 Related Classifications

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    一般には略語で表示されます：C1V1 = C2V2

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    Keywords:

    Ginsenoside Rb141753-43-9Gypenoside IIIGinsenoside Rb 1Ginsenoside Rb-1Na+/K+ ATPaseIRAKNF-κBAutophagyMitophagyHSVSodium potassium pumpInterleukin-1 receptor associated kinaseIL-1R associated kinaseNuclear factor-κBNuclear factor-kappaBMitochondrial AutophagyHerpes simplex virusInhibitorinhibitorinhibit

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