MG-132 (Synonyms: Z-Leu-Leu-Leu-al; MG132)

Name: MG-132
Brand: MedChemExpress
SKU: HY-13259
Rating: 5.0 (1998 reviews)

Cat. No.: HY-13259 Purity: 99.90%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

MG-132 (Z-Leu-Leu-Leu-al) is a potent proteasome and calpain inhibitor with IC₅₀s of 100 nM and 1.2 μM, respectively. MG-132 effectively blocks the proteolytic activity of the 26S proteasome complex. MG-132, a peptide aldehyde, also is an autophagy activator. MG-132 also induces apoptosis.

For research use only. We do not sell to patients.

CAS No. : 133407-82-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1998 publication(s) in Google Scholar

Other Forms of MG-132:

(R)-MG-132 In-stock
MG-132 (negative control) In-stock
MG-132 (GMP) Get quote

1998 Publications Citing Use of MCE MG-132

Cell Proliferation/Viability Assay

: MG-132 purchased from MedChemExpress. Usage Cited in: Cell. 2025 Feb 6;188(3):814-831.e21. [Abstract]; MG132 (42 μM; 12 h) almost completely blocks mouse embryonic development.

: MG-132 purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Dec 19;23(1):274. [Abstract]; MG132 (10 µM; 6 h) can reverse the effect of cycloheximide (CHX) (100 µg/mL) and enhance the stability of FASN protein in HCCLM3 cells.

: MG-132 purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Apr 29;23(1):86. [Abstract]; Western blot analysis of cell lysates from the indicated 293 cells treated with MG132 (20 µM) or DMSO for 6 h.

: MG-132 purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Jan 24;23(1):23. [Abstract]; MG132 (20 µM; 6 h) reverses the reduced accumulation of endogenous SRSF5 in LINC01852-overexpressing HCT116 cells and inhibited the accumulation of SRSF5 in LINC01852-depleted HT29 cells

: MG-132 purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2021 Mar;23(3):257-267. [Abstract]; Western blot analysis of DACT1 wild-type and DACT1-TdTomato fusion protein expression in both M1a and BM2 cells after treatment with either Bafilomycin A1 (10 nM) or MG-132 (20 μM) for 12 h.

: MG-132 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Jan 30;18(4):3733-3751. [Abstract]; The protein levels of SOX2 in 100 μg/mL of PS-NP-exposed Swan 71 cells treated with CHX (10 μg/mL; 4 h) and MG132 (10 μM; 6 h) or chloroquine (CQ) (15 μM; 6 h).

: MG-132 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Jul;13(7):e12468. [Abstract]; Western blotting showing the protein degradation of HEXB in HEXB-AS stable knockdown cells using MG132 (10 μM, 24 h).

: MG-132 purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2023 Jun 12;41(6):1073-1090.e12. [Abstract]; Representative immunoblot for total p65 and β-ACTIN (loading control) in BMDMs treated with GB1275 for 7 hours after 1 hour-MG132 (10 μM) pretreatment.

: MG-132 purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Aug 8;35(8):1390-1405.e8. [Abstract]; Western blot analysis of HIF-1α and IL-1β in PMs isolated from Ube2mf/f 100 and Lysm+Ube2mf/f mice, stimulated with PA for 6 h, and treated with or without MG132 (10 μM).

: MG-132 purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 May;43(5):582-612. [Abstract]; YAP1 ubiquitination degradation assay. MCF7 and SKBR3 cells were transfected with NEDD4L overexpression plasmid or siNEDD4L and treated with MG132 (20 µmol/L, 6 h). YAP1 expression was detected with WB analysis.

: MG-132 purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2023 May;26(5):751-764. [Abstract]; MG-132 (10 μg in each side; loci VPM injection) not affecting the expression of KCC2 in VPM in awake mice (h) and restoring the anesthetic-induced decreased KCC2 (i).

: MG-132 purchased from MedChemExpress. Usage Cited in: Neuro Oncol. 2023 Oct 3;25(10):1788-1801. [Abstract]; Western blotting of FAM129A and NICD1 in indicated cells. GSC was treated with MG132 (5 μM; 6 h) for harvest.

: MG-132 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2023 Mar:189:106704. [Abstract]; MG132 (10 µM) significantly reduces the degradation of YTHDC1 protein mediated by Dihydroartemisinin (DHA) in HSCs.

: MG-132 purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2023 May 10:307:116243. [Abstract]; Both MG-132 (10 μM) and Baf A1 (10 μM) markedly increase TGF-β1 protein expression in HG-stimulated SV40-MES-13 cells.

: MG-132 purchased from MedChemExpress. Usage Cited in: J Virol. 2023 Mar 30;97(3):e0198422. [Abstract]; MG-132 (200 nM; 24 h) significantly inhibits Newcastle disease virus (NDV) infection-caused degradation of β-catenin in DF-1 cells.

: MG-132 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2020 Dec 26;5(1):298. [Abstract]; Western blot analysis of YBX1 and β-tubulin in HepG2-SR cells transfected with or without si-circRNA-SORE following proteasome inhibition with MG132 treatment (20 μM) for 3 h.

: MG-132 purchased from MedChemExpress. Usage Cited in: Nat Plants. 2021 Feb;7(2):209-218. [Abstract]; In vitro degradation assays. The recombinant His-FBL17 proteins were incubated with WT extract in the presence of 1% DMSO (vehicle), 100 μM MG132 (proteasome inhibitor), or 100 μM PMSF (protease inhibitor) for 0, 1, and 2 h. The reactions were stopped by adding 5X SDS loading buffer and were subjected to western blotting using anti-His or anti-GS antibodies. The glutamine synthetase (GS) protein was used as a loading control.

: MG-132 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2020 Aug 4;32(5):107990. [Abstract]; PMs are treated with MG132 (10 μM). Endogenous YAP protein accumulated in the presence of MG132 starts from 2 h and further increases at 4 and 6 h after treatment in WT macrophages.

: MG-132 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Feb 26;38(1):101. [Abstract]; HLF and Hep3B cells are treated with Mg132 (10 μg/ml) for 4 h, total protein is extracted and subjected to western blotting using anti-Flag, anti-p21, or anti-GAPDH antibodies.

: MG-132 purchased from MedChemExpress. Usage Cited in: EMBO J. 2019 Mar 15;38(6):e100376. [Abstract]; Brcc3^+/+ and Brcc3^-/- (Abro1^+/+ and Abro1^-/-)BMDMs are treated with or without LPS for 1 h. Before LPS treatment, Brcc3^-/- (Abro1^-/-) BMDMs are pretreated with MG132 (10 μM) for 6 h to rescue the expression of ABRO1. Immunoblot analysis of NLRP3 and ABRO1 proteins in cell lysates immunoprecipitated with anti-ABRO1 antibody.

: MG-132 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2019 Jul:79:244-255. [Abstract]; Effect of autolysosome inhibitor (Chloroquine, CQ, 50 μM) or proteasome inhibitor (MG132, 5 μM) on KA-induced NLRP3 degradation. Cells are treated with the inhibitors for 0.5 h before KA (10 μM, 2 h) treatment.

: MG-132 purchased from MedChemExpress. Usage Cited in: Antioxid Redox Signal. 2019 May 20;30(15):1831-1848. [Abstract]; Tan-IIA increases the endogenous induction of Nrf2 induction and this effect is further enhanced by cotreatment with the proteasome inhibitor MG-132.

: MG-132 purchased from MedChemExpress. Usage Cited in: J Exp Bot. 2019 Sep 24;70(18):4749-4762. [Abstract]; Cell-free degradation assay of recombinant His-PhCHS protein. Recombinant His- PhCHS is purified from Escherichia coli incubated with petal crude proteins at stages and treated with specific 26S proteasome inhibitor MG132 at various time intervals. Western blot analysis was conducted using an anti-His antibody and anti-β-actin protein concentration was used as a loading control.

: MG-132 purchased from MedChemExpress. Usage Cited in: Molecules. 2019 Jan 22;24(3):393. [Abstract]; The HepG2 cells are pretreated for 5 h MG 132 which is a proteasome inhibitor. Then, the MARCH1 protein expressions in HepG2 cells treated with 0 μM, 5,0 μM SAF, and 2.5 μM MG 132 are measured by immunoblotting.

: MG-132 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2019 Feb 1;79(3):534-545. [Abstract]; Cells are transfected with GYS2 siRNA and pre-incubated with MG-132 (20 μM) for 12 h. Cell lysate are immunoprecipitated by anti-Ub and immunoblotted by anti-p53.

: MG-132 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2018 Aug 15;37(1):193. [Abstract]; Western blot is performed in HCC-LM3 cells transfected with HJURP knockdown lentivirus and treated with DMSO or MG132.

: MG-132 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2018 Oct 3;37(1):240. [Abstract]; Decrease of ERCC6 expression can be rescued with proteasome inhibitor MG132. Subconfluent SKOV3 cells are treated with FL118 and MG132 alone or in combination as shown for 8 h, followed by western blot analyses with ERCC6 antibody.

: MG-132 purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2018 Aug:34:243-255. [Abstract]; MPC1 protein expression in primary mouse hepatocytes incubated with pyruvate for 8 h in the presence of MG-132.

: MG-132 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 May 22;9(6):604. [Abstract]; Different concentrations of two classical proteasome inhibitors PS-341 and MG132 are added. AGS cells are either untreated or treated with PS-341 (25 nM) or MG132 (0.1 μM) for 24 h in the absence or presence of baf A1 (100 nM).

: MG-132 purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2018 Feb 25:146:251-259. [Abstract]; In the absence of MG132, the protein degradation pathways are intact, the Tau protein level is significantly decreased in peptide 1-treated group.

: MG-132 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2018 Aug;51(4):e12451. [Abstract]; Immunoblot analysis of CDK4 in cells treated with 4 μM of CGN for 24 hours in the presence and absence of MG132.

: MG-132 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2018 Oct:156:511-523. [Abstract]; The accumulated poly-ubiquitinated protein is detected by Western blotting after J-Lat 10.6 cells are treated with DMSO, PR-957 (100 nM), PR-957 (150 nM) or MG132 (500 nM) for 48 h.

: MG-132 purchased from MedChemExpress. Usage Cited in: Mol Plant Pathol. 2018 Dec;19(12):2623-2634. [Abstract]; Destabilization of BRC1 mediated by SWP1 is inhibited by proteasome inhibitors Epoxomicin and MG132.

: MG-132 purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Apr 19:9:377. [Abstract]; Immunoblot levels of highly molecular weight (HMW)-ubiquitinated proteins and pSer129-α-syn after inhibiting the ubiquitin-proteasome system (UPS) by various concentrations of MG132 (0.25, 0.5, and 1 μM) in SH-SY5Y cells.

: MG-132 purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2018 Oct;1864(10):3322-3338. [Abstract]; The neonatal rat cardiomyocytes (NRCMs) are treated with MG132 (10 μM). The protein expression level of PPARα in the indicated group.

: MG-132 purchased from MedChemExpress. Usage Cited in: Cell Cycle. 2018;17(13):1591-1601. [Abstract]; The protein level of CCNB1 in different groups is analyzed by Western blot; MG132 significantly increases the protein level of CCNB1 in oocytes from CRS group mice. Western blotting showing the reduced expression of securin is rescued by MG132 in CRS group mouse oocytes.

: MG-132 purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2018 Dec:104:69-78. [Abstract]; Western analysis of proteins expression with treatment of IKK-16 or MG-132.

: MG-132 purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2018 Nov;16(5):5900-5906. [Abstract]; Treatment with the 26S proteasome inhibitor, MG 132 (10 µM), rescues the downregulation of NEK 8 in the pVHL overexpressing SGC 7901 cells.

: MG-132 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2017 Dec 1:410:112-123. [Abstract]; Immunoprecipitation of ubiquitin from lysates of U-2 OS cells expressing CHOP after treatment with different concentrations of MG7 for 48 h. Cells are incubated with MG132 (20 mM) for 4 h before harvest. Cell lysates are immunoblotted with the indicated antibodies.

: MG-132 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2017 Jun 7;7(1):2929. [Abstract]; p53 and Cell apoptosis. MCF7 and MDA-MB-231 cells are treated with 80 μM ω-3 FFAs, 20 μM ATRA alone or in combination for 48 h. The expression of PARP and p53 protein. β-Actin is used as an internal control.

: MG-132 purchased from MedChemExpress. Usage Cited in: J Inorg Biochem. 2017 Oct:175:92-100. [Abstract]; TPEN-triggered PML-RARα degradation in NB4 cells is reversed by MG-132 treatment. NB4 cells are treated with 5 μM TPEN with or without the presence of 1 μM MG-132 for the indicated durations (0–12 h) and then lysed. The lysates are analyzed for PML-RARα and RARα protein levels by western blotting.

: MG-132 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2017 Sep 2;490(4):1168-1175. [Abstract]; Treatment of CHO-K1 cells with proteasome inhibitor, MG-132 results in elevated SR-B1 protein levels. The cells with MG-132, a potent proteasome inhibitor that inhibit ubiquitin/proteasome-dependent protein degradation, and MG-132 treatment significantly enhances cellular SR-B1 protein level.

: MG-132 purchased from MedChemExpress. Usage Cited in: Université de Montréal. Octobre 2017.; Unsynchronized control and ARF6 cells are treated with DMSO or MG132 (10 μM) for 1h or 4h, lysed and total ubiquitinated proteins are determined using western blot (n=3).

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 100 nM (Proteasome), 1.2 μM (Calpain)^[1]^[3]

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	< 10 3 Compound: MG132	Cytotoxicity against african green monkey COS7 cells by XTT method Cytotoxicity against african green monkey COS7 cells by XTT method	[PMID: 18088097]
COS-7	IC₅₀	< 10 3 Compound: MG132	Cytotoxicity against african green monkey COS7 cells by XTT method Cytotoxicity against african green monkey COS7 cells by XTT method	[PMID: 18088097]
COS-7	IC₅₀	<10 3 Compound: MG132	Cytotoxicity against african green monkey COS7 cells by XTT method Cytotoxicity against african green monkey COS7 cells by XTT method	[PMID: 18088097]
HCT-116	IC₅₀	0.82 3 Compound: MG132	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31312413]
HCT-116	IC₅₀	0.82 3 Compound: MG132	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31312413]
HEK293	IC₅₀	0.009 3 Compound: MG132	Inhibition of chymotrypsin-like activity of proteasome beta-5 subunit in HEK293 cells using Suc-LLVY-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta5 assay Inhibition of chymotrypsin-like activity of proteasome beta-5 subunit in HEK293 cells using Suc-LLVY-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta5 assay	[PMID: 23540790]
HEK293	IC₅₀	0.009 3 Compound: MG132	Inhibition of chymotrypsin-like activity of proteasome beta-5 subunit in HEK293 cells using Suc-LLVY-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta5 assay Inhibition of chymotrypsin-like activity of proteasome beta-5 subunit in HEK293 cells using Suc-LLVY-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta5 assay	[PMID: 23540790]
HEK293	IC₅₀	0.6 3 Compound: MG132	Inhibition of postacid activity of 20s proteasome beta-1 subunit in HEK293 cells using Z-nLPnLD-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta1 assay Inhibition of postacid activity of 20s proteasome beta-1 subunit in HEK293 cells using Z-nLPnLD-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta1 assay	[PMID: 23540790]
HEK293	IC₅₀	0.6 3 Compound: MG132	Inhibition of postacid activity of 20s proteasome beta-1 subunit in HEK293 cells using Z-nLPnLD-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta1 assay Inhibition of postacid activity of 20s proteasome beta-1 subunit in HEK293 cells using Z-nLPnLD-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta1 assay	[PMID: 23540790]
HL-60	IC₅₀	0.068 3 Compound: MG132	Inhibitory concentration to inhibit chymotrypsin-like activity of 20S proteasome prepared from human leukemia HL-60 cells was determined Inhibitory concentration to inhibit chymotrypsin-like activity of 20S proteasome prepared from human leukemia HL-60 cells was determined	[PMID: 15780623]
Huh-7	IC₅₀	< 10 3 Compound: MG132	Cytotoxicity against human HuH7 cells by XTT method Cytotoxicity against human HuH7 cells by XTT method	[PMID: 18088097]
HL-60	IC₅₀	1.4 3 Compound: MG132	Inhibitory concentration to inhibit the PGPH activity of 20S proteasome prepared from human leukemia HL-60 cells was determined Inhibitory concentration to inhibit the PGPH activity of 20S proteasome prepared from human leukemia HL-60 cells was determined	[PMID: 15780623]
MCF-10A	IC₅₀	0.29 3 Compound: MG-132	Growth inhibition of human MCF10A cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MCF10A cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
HL-60	IC₅₀	4.5 3 Compound: MG132	Inhibitory concentration to inhibit trypsin-like activity of 20S proteasome from human leukemia HL-60 cells was determined Inhibitory concentration to inhibit trypsin-like activity of 20S proteasome from human leukemia HL-60 cells was determined	[PMID: 15780623]
MCF7	IC₅₀	0.13 3 Compound: MG-132	Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
MDA-MB-231	IC₅₀	0.18 3 Compound: MG-132	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
Huh-7	IC₅₀	<10 3 Compound: MG132	Cytotoxicity against human HuH7 cells by XTT method Cytotoxicity against human HuH7 cells by XTT method	[PMID: 18088097]
HUVEC	IC₅₀	0.6 3 Compound: 1, MG-132	Inhibition of TNFalpha stimulated human NF-kappaB-mediated beta lactamase gene expression in HUVEC Inhibition of TNFalpha stimulated human NF-kappaB-mediated beta lactamase gene expression in HUVEC	[PMID: 18024113]
NCI-H23	IC₅₀	0.48 3 Compound: MG-132	Cytotoxicity against human NCI-H23 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human NCI-H23 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30964987]
MCF-10A	IC₅₀	0.29 3 Compound: MG-132	Growth inhibition of human MCF10A cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MCF10A cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
NCI-H727	IC₅₀	0.98 3 Compound: MG-132	Cytotoxicity against human NCI-H727 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human NCI-H727 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30964987]
MCF7	IC₅₀	0.3 3 Compound: MG-132	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 18598018]
NCI-H929	IC₅₀	0.17 3 Compound: MG-132	Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay	[PMID: 24625088]
MCF7	IC₅₀	0.13 3 Compound: MG-132	Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
OCI-Ly3	IC₅₀	> 10 3 Compound: 1, MG-132	Cytotoxicity against NF-kappaB overexpressing human OCI-Ly3 cells after 4 hrs Cytotoxicity against NF-kappaB overexpressing human OCI-Ly3 cells after 4 hrs	[PMID: 18024113]
MDA-MB-231	IC₅₀	0.25 3 Compound: MG-132	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 18598018]
RAW264.7	IC₅₀	0.08 6 Compound: MG132	Inhibition of LPS and IFN-gamma-stimulated nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess method Inhibition of LPS and IFN-gamma-stimulated nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess method	[PMID: 26351042]
RAW264.7	IC₅₀	0.08 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent method	[PMID: 22620677]
MDA-MB-231	IC₅₀	0.18 3 Compound: MG-132	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
HCT-116	IC₅₀	0.82 3 Compound: MG132	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31312413]
NCI-H23	IC₅₀	0.48 3 Compound: MG-132	Cytotoxicity against human NCI-H23 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human NCI-H23 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30964987]
RAW264.7	IC₅₀	0.1 3 Compound: MG132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 18 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 18 hrs by Griess method	[PMID: 33422907]
NCI-H727	IC₅₀	0.98 3 Compound: MG-132	Cytotoxicity against human NCI-H727 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human NCI-H727 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30964987]
HEK293	IC₅₀	0.009 3 Compound: MG132	Inhibition of chymotrypsin-like activity of proteasome beta-5 subunit in HEK293 cells using Suc-LLVY-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta5 assay Inhibition of chymotrypsin-like activity of proteasome beta-5 subunit in HEK293 cells using Suc-LLVY-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta5 assay	[PMID: 23540790]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 24219809]
NCI-H929	IC₅₀	0.17 3 Compound: MG-132	Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay	[PMID: 24625088]
HEK293	IC₅₀	0.6 3 Compound: MG132	Inhibition of postacid activity of 20s proteasome beta-1 subunit in HEK293 cells using Z-nLPnLD-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta1 assay Inhibition of postacid activity of 20s proteasome beta-1 subunit in HEK293 cells using Z-nLPnLD-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta1 assay	[PMID: 23540790]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method	[PMID: 23819871]
OCI-Ly3	IC₅₀	>10 3 Compound: 1, MG-132	Cytotoxicity against NF-kappaB overexpressing human OCI-Ly3 cells after 4 hrs Cytotoxicity against NF-kappaB overexpressing human OCI-Ly3 cells after 4 hrs	[PMID: 18024113]
HL-60	IC₅₀	0.068 3 Compound: MG132	Inhibitory concentration to inhibit chymotrypsin-like activity of 20S proteasome prepared from human leukemia HL-60 cells was determined Inhibitory concentration to inhibit chymotrypsin-like activity of 20S proteasome prepared from human leukemia HL-60 cells was determined	[PMID: 15780623]
RAW264.7	IC₅₀	0.1 3 Compound: MG132	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method	[PMID: 24597894]
PC-3	IC₅₀	600 1 Compound: 5b	Antiproliferative activity against human PC3 cell line Antiproliferative activity against human PC3 cell line	[PMID: 16686537]
HL-60	IC₅₀	1.4 3 Compound: MG132	Inhibitory concentration to inhibit the PGPH activity of 20S proteasome prepared from human leukemia HL-60 cells was determined Inhibitory concentration to inhibit the PGPH activity of 20S proteasome prepared from human leukemia HL-60 cells was determined	[PMID: 15780623]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells after 18 hrs by griess reagent based plate reader analysis Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells after 18 hrs by griess reagent based plate reader analysis	[PMID: 24697496]
RAW264.7	IC₅₀	2.6 3 Compound: MG132	Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated with compound for 2 hrs followed by LPS stimulation for 12 hrs by Griess reagent based analysis Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated with compound for 2 hrs followed by LPS stimulation for 12 hrs by Griess reagent based analysis	[PMID: 36607819]
HL-60	IC₅₀	4.5 3 Compound: MG132	Inhibitory concentration to inhibit trypsin-like activity of 20S proteasome from human leukemia HL-60 cells was determined Inhibitory concentration to inhibit trypsin-like activity of 20S proteasome from human leukemia HL-60 cells was determined	[PMID: 15780623]
RAW264.7	IC₅₀	0.15 3 Compound: MG-132	Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 25 uM preincubated with compound followed by LPS challenge measured after 18 hrs by Griess method Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 25 uM preincubated with compound followed by LPS challenge measured after 18 hrs by Griess method	[PMID: 26757019]
RAW264.7	IC₅₀	0.16 3 Compound: MG-132	Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent based assay Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent based assay	[PMID: 30629435]
RAW264.7	IC₅₀	0.15 3 Compound: MG-132	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells measured after 18 hrs by Griess reagent based multilabel plate reader analysis Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells measured after 18 hrs by Griess reagent based multilabel plate reader analysis	[PMID: 23327668]
RAW264.7	IC₅₀	0.17 6 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFNgamma-induced NO production after 5 mins by Greiss method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFNgamma-induced NO production after 5 mins by Greiss method	[PMID: 22277277]
HUVEC	IC₅₀	0.6 3 Compound: 1, MG-132	Inhibition of TNFalpha stimulated human NF-kappaB-mediated beta lactamase gene expression in HUVEC Inhibition of TNFalpha stimulated human NF-kappaB-mediated beta lactamase gene expression in HUVEC	[PMID: 18024113]
RAW264.7	IC₅₀	0.16 3 Compound: MG-132	Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent based assay Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent based assay	[PMID: 30629435]
RAW264.7	IC₅₀	0.18 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay	[PMID: 27203291]
RAW264.7	IC₅₀	0.17 6 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 5 mins by Greiss method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 5 mins by Greiss method	[PMID: 22277277]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method	[PMID: 23819871]
RAW264.7	IC₅₀	0.17 6 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFNgamma-induced NO production after 5 mins by Greiss method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFNgamma-induced NO production after 5 mins by Greiss method	[PMID: 22277277]
Huh-7	IC₅₀	< 10 3 Compound: MG132	Cytotoxicity against human HuH7 cells by XTT method Cytotoxicity against human HuH7 cells by XTT method	[PMID: 18088097]
RAW264.7	IC₅₀	0.18 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay	[PMID: 27203291]
RAW264.7	IC₅₀	0.1 3 Compound: MG132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 18 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 18 hrs by Griess method	[PMID: 33422907]
RAW264.7	IC₅₀	0.2 3 Compound: MG132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated iNOS activity preincubated with compound for 24 hrs followed by LPS stimulation for 2 hrs and measured 2 hrs after NADPH addition by fluorescence based assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated iNOS activity preincubated with compound for 24 hrs followed by LPS stimulation for 2 hrs and measured 2 hrs after NADPH addition by fluorescence based assay	[PMID: 33454546]
RAW264.7	IC₅₀	0.2 3 Compound: MG132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated iNOS activity preincubated with compound for 24 hrs followed by LPS stimulation for 2 hrs and measured 2 hrs after NADPH addition by fluorescence based assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated iNOS activity preincubated with compound for 24 hrs followed by LPS stimulation for 2 hrs and measured 2 hrs after NADPH addition by fluorescence based assay	[PMID: 33454546]
RAW264.7	IC₅₀	0.08 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent method	[PMID: 22620677]
RAW264.7	IC₅₀	0.2 3 Compound: MG132	Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated with compound for 24 hrs followed by LPS stimulation and measured after 2 hrs by Griess reagent-based assay Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated with compound for 24 hrs followed by LPS stimulation and measured after 2 hrs by Griess reagent-based assay	[PMID: 33454546]
RAW264.7	IC₅₀	0.2 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production preincubated for 1 hr followed by LPS stimulation measured after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production preincubated for 1 hr followed by LPS stimulation measured after 18 hrs by Griess assay	[PMID: 29338260]
RAW264.7	IC₅₀	0.2 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production preincubated for 1 hr followed by LPS stimulation measured after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production preincubated for 1 hr followed by LPS stimulation measured after 18 hrs by Griess assay	[PMID: 29338260]
RAW264.7	IC₅₀	0.35 3 Compound: MG-132	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs	[PMID: 21044847]
RAW264.7	IC₅₀	0.439 3 Compound: MG-132	Antiinflammatory against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production Antiinflammatory against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production	[PMID: 21561060]
MCF-10A	IC₅₀	0.29 3 Compound: MG-132	Growth inhibition of human MCF10A cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MCF10A cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
RAW264.7	IC₅₀	0.35 3 Compound: MG-132	Inhibition of NO production in Interferon gamma-stimulated mouse RAW264.7 cells after 18 hrs Inhibition of NO production in Interferon gamma-stimulated mouse RAW264.7 cells after 18 hrs	[PMID: 21044847]
RAW264.7	IC₅₀	0.08 6 Compound: MG132	Inhibition of LPS and IFN-gamma-stimulated nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess method Inhibition of LPS and IFN-gamma-stimulated nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess method	[PMID: 26351042]
RAW264.7	IC₅₀	0.63 3 Compound: MG132	Toxicity against mouse RAW264.7 cells by XTT assay Toxicity against mouse RAW264.7 cells by XTT assay	[PMID: 22858297]
MCF7	IC₅₀	0.13 3 Compound: MG-132	Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
RAW264.7	IC₅₀	0.1 3 Compound: MG132	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method	[PMID: 24597894]
MCF7	IC₅₀	0.3 3 Compound: MG-132	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 18598018]
RAW264.7	IC₅₀	0.35 3 Compound: MG-132	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs	[PMID: 21044847]
RAW264.7	IC₅₀	2.6 3 Compound: MG132	Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated with compound for 2 hrs followed by LPS stimulation for 12 hrs by Griess reagent based analysis Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated with compound for 2 hrs followed by LPS stimulation for 12 hrs by Griess reagent based analysis	[PMID: 36607819]
MDA-MB-231	IC₅₀	0.18 3 Compound: MG-132	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay	[PMID: 24153206]
RAW264.7	IC₅₀	0.15 3 Compound: MG-132	Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 25 uM preincubated with compound followed by LPS challenge measured after 18 hrs by Griess method Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 25 uM preincubated with compound followed by LPS challenge measured after 18 hrs by Griess method	[PMID: 26757019]
SW480	IC₅₀	29.5 3 Compound: MG132	Inhibition of NFkappaB transcription in human SW480 cells at by luciferase reporter gene assay Inhibition of NFkappaB transcription in human SW480 cells at by luciferase reporter gene assay	[PMID: 26841168]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 24219809]
MDA-MB-231	IC₅₀	0.25 3 Compound: MG-132	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 18598018]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells after 18 hrs by griess reagent based plate reader analysis Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells after 18 hrs by griess reagent based plate reader analysis	[PMID: 24697496]
NCI-H23	IC₅₀	0.48 3 Compound: MG-132	Cytotoxicity against human NCI-H23 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human NCI-H23 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30964987]
NCI-H727	IC₅₀	0.98 3 Compound: MG-132	Cytotoxicity against human NCI-H727 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human NCI-H727 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30964987]
RAW264.7	IC₅₀	0.15 3 Compound: MG-132	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells measured after 18 hrs by Griess reagent based multilabel plate reader analysis Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells measured after 18 hrs by Griess reagent based multilabel plate reader analysis	[PMID: 23327668]
NCI-H929	IC₅₀	0.17 3 Compound: MG-132	Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay	[PMID: 24625088]
RAW264.7	IC₅₀	0.63 3 Compound: MG132	Toxicity against mouse RAW264.7 cells by XTT assay Toxicity against mouse RAW264.7 cells by XTT assay	[PMID: 22858297]
SW480	IC₅₀	29.5 3 Compound: MG132	Inhibition of NFkappaB transcription in human SW480 cells at by luciferase reporter gene assay Inhibition of NFkappaB transcription in human SW480 cells at by luciferase reporter gene assay	[PMID: 26841168]
OCI-Ly3	IC₅₀	> 10 3 Compound: 1, MG-132	Cytotoxicity against NF-kappaB overexpressing human OCI-Ly3 cells after 4 hrs Cytotoxicity against NF-kappaB overexpressing human OCI-Ly3 cells after 4 hrs	[PMID: 18024113]
PC-3	IC₅₀	600 1 Compound: 5b	Antiproliferative activity against human PC3 cell line Antiproliferative activity against human PC3 cell line	[PMID: 16686537]
RAW264.7	IC₅₀	0.08 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent method	[PMID: 22620677]
RAW264.7	IC₅₀	0.08 6 Compound: MG132	Inhibition of LPS and IFN-gamma-stimulated nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess method Inhibition of LPS and IFN-gamma-stimulated nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess method	[PMID: 26351042]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method	[PMID: 23819871]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 24219809]
RAW264.7	IC₅₀	0.1 3 Compound: MG-132	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells after 18 hrs by griess reagent based plate reader analysis Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells after 18 hrs by griess reagent based plate reader analysis	[PMID: 24697496]
RAW264.7	IC₅₀	0.1 3 Compound: MG132	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method	[PMID: 24597894]
RAW264.7	IC₅₀	0.1 3 Compound: MG132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 18 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 18 hrs by Griess method	[PMID: 33422907]
RAW264.7	IC₅₀	0.15 3 Compound: MG-132	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells measured after 18 hrs by Griess reagent based multilabel plate reader analysis Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells measured after 18 hrs by Griess reagent based multilabel plate reader analysis	[PMID: 23327668]
RAW264.7	IC₅₀	0.15 3 Compound: MG-132	Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 25 uM preincubated with compound followed by LPS challenge measured after 18 hrs by Griess method Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 25 uM preincubated with compound followed by LPS challenge measured after 18 hrs by Griess method	[PMID: 26757019]
RAW264.7	IC₅₀	0.16 3 Compound: MG-132	Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent based assay Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-stimulated NO production after 18 hrs by Griess reagent based assay	[PMID: 30629435]
RAW264.7	IC₅₀	0.17 6 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFNgamma-induced NO production after 5 mins by Greiss method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFNgamma-induced NO production after 5 mins by Greiss method	[PMID: 22277277]
RAW264.7	IC₅₀	0.17 6 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 5 mins by Greiss method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 5 mins by Greiss method	[PMID: 22277277]
RAW264.7	IC₅₀	0.18 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay	[PMID: 27203291]
RAW264.7	IC₅₀	0.2 3 Compound: MG-132	Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production preincubated for 1 hr followed by LPS stimulation measured after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production preincubated for 1 hr followed by LPS stimulation measured after 18 hrs by Griess assay	[PMID: 29338260]
RAW264.7	IC₅₀	0.2 3 Compound: MG132	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated iNOS activity preincubated with compound for 24 hrs followed by LPS stimulation for 2 hrs and measured 2 hrs after NADPH addition by fluorescence based assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated iNOS activity preincubated with compound for 24 hrs followed by LPS stimulation for 2 hrs and measured 2 hrs after NADPH addition by fluorescence based assay	[PMID: 33454546]
RAW264.7	IC₅₀	0.2 3 Compound: MG132	Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated with compound for 24 hrs followed by LPS stimulation and measured after 2 hrs by Griess reagent-based assay Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated with compound for 24 hrs followed by LPS stimulation and measured after 2 hrs by Griess reagent-based assay	[PMID: 33454546]
RAW264.7	IC₅₀	0.35 3 Compound: MG-132	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs	[PMID: 21044847]
RAW264.7	IC₅₀	0.35 3 Compound: MG-132	Inhibition of NO production in Interferon gamma-stimulated mouse RAW264.7 cells after 18 hrs Inhibition of NO production in Interferon gamma-stimulated mouse RAW264.7 cells after 18 hrs	[PMID: 21044847]
RAW264.7	IC₅₀	0.439 3 Compound: MG-132	Antiinflammatory against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production Antiinflammatory against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production	[PMID: 21561060]
RAW264.7	IC₅₀	0.63 3 Compound: MG132	Toxicity against mouse RAW264.7 cells by XTT assay Toxicity against mouse RAW264.7 cells by XTT assay	[PMID: 22858297]
RAW264.7	IC₅₀	2.6 3 Compound: MG132	Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated with compound for 2 hrs followed by LPS stimulation for 12 hrs by Griess reagent based analysis Anti-inflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated with compound for 2 hrs followed by LPS stimulation for 12 hrs by Griess reagent based analysis	[PMID: 36607819]
SW480	IC₅₀	29.5 3 Compound: MG132	Inhibition of NFkappaB transcription in human SW480 cells at by luciferase reporter gene assay Inhibition of NFkappaB transcription in human SW480 cells at by luciferase reporter gene assay	[PMID: 26841168]

In Vitro

MG-132 (Z-Leu-Leu-Leu-al) initiates neurite outgrowth in PC12 cells at a low concentration (30 nM) and is a very strong inhibitor of 20S proteasome^[3].
MG-132 (10 μM; 1 hour) reverses the effects of TNF- α on I κ B degradation and NF-κ B activation in A549 cells^[4].
MG-132 (0.75-5 μM; 24 hours) potently induces p53-dependent apoptosis in KIM-2 cells by 26S proteasome inhibition^[5].
MG-132 (10-40 μM; 24 hours) significantly reduces the viability of C6 glioma cells in both time- and concentration-dependent manners and shows the IC₅₀ of 18.5 μM at 24 hours^[6].
MG-132 (18.5 μM; 24 hours) induces down-regulation of anti-apoptotic proteins Bcl-2 and XIAP and up-regulates expression of pro-apoptotic protein Bax and caspase-3^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	C6 glioma cells
Concentration:	10, 20, 30, 40 μM
Incubation Time:	24 hours
Result:	Significantly reduced the viability of C6 glioma cells beginning at 6 h in both time- and concentration-dependent manners and showed the IC₅₀ of 18.5 μM at 24 hours.

Western Blot Analysis^[3]

Cell Line:	A549 cells
Concentration:	10 μM
Incubation Time:	1 hour
Result:	Reversed the effects of TNF-α on IκB degradation and resulted in a reversal of TNF-α-induced NF-κB activation.

In Vivo

MG132 (10 mg/kg; i.p.; daily for 25 days starting 5 days after EC9706 cells injection) significantly inhibits tumor growth of the EC9706 xenograft without causing toxicity to mice^[7].
MG-132 (1 mg/kg; i.v.; twice a week for 4 weeks) shows potent tumor inhibitory effect against mice bearing HeLa tumors^[8].
MG-132 (1-10 μg/kg/24 hours; subcutaneously implanted osmotic pumps; for 8 days) greatly increases the expression levels of β-dystroglycan, α-dystroglycan, α-sarcoglycan, and dystrophin in skeletal muscle lysates in mice (six-month-old male C57BL/10ScSn DMD mdx mice)^[9].

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

MG-132, characterized by a short plasma half-life and poor blood-brain barrier permeability, is administered via stereotactic intranigral injection (0.4 μg) to achieve sustained local proteasome inhibition, successfully inducing Parkinson's disease-like pathology in the substantia nigra^[10].

Induction of Parkinson’s Disease^[10]

Background

MG-132 induces Parkinson's disease-like pathology by inhibiting the ubiquitin-proteasome system (UPS), leading to proteolytic stress, apoptosis of dopaminergic neurons, and a consequent depletion of striatal dopamine^[10].

Specific Modeling Methods

Mice: C57BL/6 black mice
Administration: MG-132 (0.4 μg in 4 μL) • stereotaxically injected into the substantia nigra at the target site (Bregma AP, 3.2 mm, ML, 2.0 mm, DV, 4.7 mm) in the right side

Note

(1) MG-132 is dissolved at a concentration of 0.1 μg/μL in a vehicle of 1% DMSO in PBS.
(2) Injection should be extremely slow (e.g., 0.5 μL/min), and the needle should be left in place for several minutes after injection to prevent fluid reflux and mechanical damage that could affect model specificity.
(3) The control side must use the same solvent as the drug solution (1% DMSO in PBS) to eliminate the influence of the solvent itself.

Modeling Indicators

Molecular changes: Striatal dopamine and DOPAC depletion
Histology analysis: Nigral tyrosine hydroxylase-positive (TH+) neurons loss

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5- to 6-weeks old female athymic nude mice (EC9706 xenograft)^[7]
Dosage:	10 mg/kg
Administration:	I.p.; daily for 25 days starting 5 days after EC9706 cells injection
Result:	Significantly inhibited tumor growth of the EC9706 xenograft without causing toxicity to the mice.

Animal Model:	Five-week-old female C.B-17/lcr-scid/scidJcl mice (bearing HeLa cells)^[8]
Dosage:	1 mg/kg
Administration:	Intravenous injection; twice a week for 4 weeks
Result:	The growth inhibition rates in HeLa tumors was 49% compared to the control.

Molecular Weight

475.62

Formula

C₂₆H₄₁N₃O₅

CAS No.

133407-82-6

Appearance

Solid

Color

White to yellow

SMILES

O=C(OCC1=CC=CC=C1)N[C@H](C(N[C@@H](CC(C)C)C(N[C@H](C([H])=O)CC(C)C)=O)=O)CC(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (105.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1025 mL	10.5126 mL	21.0252 mL
5 mM	0.4205 mL	2.1025 mL	4.2050 mL
10 mM	0.2103 mL	1.0513 mL	2.1025 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (3.51 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.67 mg/mL (3.51 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (3.51 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (285 KB) SDS (396 KB)

COA (246 KB) HNMR (280 KB) RP-HPLC (215 KB) MS (69 KB) Elemental Analysis Report (104 KB)

Handling Instructions (2659 KB)

References

[1]. Harhouri K, et al. MG132-induced progerin clearance is mediated by autophagy activation and splicing regulation. EMBO Mol Med. 2017 Sep;9(9):1294-1313. [Content Brief]

[2]. Fan WH, et al. Proteasome inhibitor MG-132 induces C6 glioma cell apoptosis via oxidative stress. Acta Pharmacol Sin. 2011 May;32(5):619-25. [Content Brief]

[3]. Tsubuki S, et al. Differential inhibition of calpain and proteasome activities by peptidyl aldehydes of di-leucine and tri-leucine. J Biochem. 1996 Mar;119(3):572-6. [Content Brief]

[4]. Fiedler MA, et al. Inhibition of TNF-alpha-induced NF-kappaB activation and IL-8 release in A549 cells with the proteasome inhibitor MG-132. Am J Respir Cell Mol Biol. 1998 Aug;19(2):259-68. [Content Brief]

[5]. MacLaren AP, et al. p53-dependent apoptosis induced by proteasome inhibition in mammary epithelial cells. Cell Death Differ. 2001 Mar;8(3):210-8. [Content Brief]

[6]. Han YH, et al. The effect of MG132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH. Oncol Rep. 2009 Jul;22(1):215-21. [Content Brief]

[7]. Dang L, et al. Proteasome inhibitor MG132 inhibits the proliferation and promotes the cisplatin-inducedapoptosis of human esophageal squamous cell carcinoma cells. Int J Mol Med. 2014 May;33(5):1083-8. [Content Brief]

[8]. Matsumoto Y, et al. Enhanced efficacy against cervical carcinomas through polymeric micelles physically incorporating theproteasome inhibitor MG132. Cancer Sci. 2016 Jun;107(6):773-81. [Content Brief]

[9]. Bonuccelli G, et al. Proteasome inhibitor (MG-132) treatment of mdx mice rescues the expression and membrane localization of dystrophin and dystrophin-associated proteins. Am J Pathol. 2003 Oct;163(4):1663-75. [Content Brief]

[10]. Sun F, et al. Proteasome inhibitor MG-132 induces dopaminergic degeneration in cell culture and animal models. Neurotoxicology. 2006 Sep;27(5):807-15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1025 mL	10.5126 mL	21.0252 mL	52.5630 mL
	5 mM	0.4205 mL	2.1025 mL	4.2050 mL	10.5126 mL
	10 mM	0.2103 mL	1.0513 mL	2.1025 mL	5.2563 mL
	15 mM	0.1402 mL	0.7008 mL	1.4017 mL	3.5042 mL
	20 mM	0.1051 mL	0.5256 mL	1.0513 mL	2.6281 mL
	25 mM	0.0841 mL	0.4205 mL	0.8410 mL	2.1025 mL
	30 mM	0.0701 mL	0.3504 mL	0.7008 mL	1.7521 mL
	40 mM	0.0526 mL	0.2628 mL	0.5256 mL	1.3141 mL
	50 mM	0.0421 mL	0.2103 mL	0.4205 mL	1.0513 mL
	60 mM	0.0350 mL	0.1752 mL	0.3504 mL	0.8760 mL
	80 mM	0.0263 mL	0.1314 mL	0.2628 mL	0.6570 mL
	100 mM	0.0210 mL	0.1051 mL	0.2103 mL	0.5256 mL

MG-132 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MG-132133407-82-6Z-Leu-Leu-Leu-al MG132MG132MG 132ProteasomeAutophagyApoptosispeptidealdehydeproteolytic26SproteasomecomplexcalpainInhibitorinhibitorinhibit

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Product Name			Requested Quantity *
Applicant Name *			Salutation
Email Address *			Phone Number *
Department			Organization Name *
City			State
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

MG-132 (Synonyms: Z-Leu-Leu-Leu-al; MG132)

Customer Review

Other Forms of MG-132:

MG-132 Related Antibodies

1998 Publications Citing Use of MCE MG-132

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

MG-132 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

MG-132 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report