(R)-MG-132 (Synonyms: (S,R,S)-(-)-MG-132; Z-Leu-D-Leu-Leu-al)

Name: (R)-MG-132
Brand: MedChemExpress
SKU: HY-13259C
Rating: 5.0 (16 reviews)

Cat. No.: HY-13259C Purity: 99.97%: Data Sheet
COA

SDS
Handling Instructions
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(R)-MG-132 ((S,R,S)-(-)-MG-132) is the enantiomer of MG-132. (R)-MG-132 is a proteasome inhibitor with weaker cell cytotoxicity than MG-132.

For research use only. We do not sell to patients.

CAS No. : 1211877-36-9

Size	Stock	Quantity
Free Sample (0.1 - 0.2 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 16 publication(s) in Google Scholar

Other Forms of (R)-MG-132:

MG-132 In-stock
MG-132 (negative control) In-stock

16 Publications Citing Use of MCE (R)-MG-132

: (R)-MG-132 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 8:e14512. [Abstract]; Western blotting analysis showed the expression of specified proteins in HSV-1-infected control or PBLD-silenced HeLa cells treated with Z-VAD-FMK (20 µM), MG132 (10 µg/mL), or CQ (50 nM) for 12 h.

: (R)-MG-132 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 8:e14512. [Abstract]; The HeLa cells were transfected with the indicated vectors for 36 h and treated with MG132, followed by co-IP analysis.

: (R)-MG-132 purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 Nov 18;24(1):382. [Abstract]; MG132 (20 μM; 8 h). Western blot analysis showed the levels of DDX20 in 293T cells with control (SCR shRNA) or knockdown of TRIM25. The relative DDX20 protein level was illustrated graphically.

: (R)-MG-132 purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 Nov 18;24(1):382. [Abstract]; Effect of HA-DAPK overexpression on the ubiquitination of endogenous DDX20 in 293T cells. Whole cell lysates (containing HA-DAPK) were immunoprecipitated using an anti-DDX20 antibody and blotted with an anti-Ubiquitin antibody. The working concentration of MG132 was 20 μM for 8 h.

: (R)-MG-132 purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Mar;43(13):931-943. [Abstract]; MG132 (10 μM; 12 h). TMEM65 decreased YWHAZ ubiquitination and increased its protein level.

: (R)-MG-132 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2020 Oct;27(10):875-885. [Abstract]; Immunoblot analysis of 293T cells transfected with indicated plasmids encoding wild-type or mutant NS5-HAs was performed using antibodies against HA, hSTAT2, and GAPDH, and the cells were incubated with or without proteasome inhibitor MG132 (10 nM; 36 h).

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Description

(R)-MG-132 ((S,R,S)-(-)-MG-132) is the enantiomer of MG-132. (R)-MG-132 is a proteasome inhibitor with weaker cell cytotoxicity than MG-132^[1].

IC₅₀ & Target

Proteasome^[1]

In Vitro

(R)-MG-132, the stereoisomer of MG-132, is studied as a potential inhibitor of chymotrypsin-like, trypsin-like, and peptidylglutamyl peptide hydrolyzing activities of proteasome^[1].
MG-132 and (R)-MG-132 are investigated for inhibition of ChTL, trypsin-like (TL) and peptidylglutamyl peptide hydrolyzing (PGPH) activities of purified 20S proteasomes isolated from human erythrocytes. For MG-132, the IC₅₀s of 0.89 μM, 104.43 μM, and 5.7 μM for ChTL, TL, and PGPH, respectively. For (R)-MG-132, the IC₅₀s of 0.22 μM, 34.4 μM, and 2.95 μM for ChTL, TL, and PGPH, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

475.62

Formula

C₂₆H₄₁N₃O₅

CAS No.

1211877-36-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@H](CC(C)C)C(N[C@@H](CC(C)C)C=O)=O)[C@H](CC(C)C)NC(OCC1=CC=CC=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (210.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1025 mL	10.5126 mL	21.0252 mL
5 mM	0.4205 mL	2.1025 mL	4.2050 mL
10 mM	0.2103 mL	1.0513 mL	2.1025 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.26 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (1.75 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (142 KB) RP-HPLC (220 KB)

Handling Instructions (2659 KB)

References

[1]. Mroczkiewicz M, et al. Studies of the synthesis of all stereoisomers of MG-132 proteasome inhibitors in the tumor targeting approach. J Med Chem. 2010 Feb 25;53(4):1509-18. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1025 mL	10.5126 mL	21.0252 mL	52.5630 mL
	5 mM	0.4205 mL	2.1025 mL	4.2050 mL	10.5126 mL
	10 mM	0.2103 mL	1.0513 mL	2.1025 mL	5.2563 mL
	15 mM	0.1402 mL	0.7008 mL	1.4017 mL	3.5042 mL
	20 mM	0.1051 mL	0.5256 mL	1.0513 mL	2.6281 mL
	25 mM	0.0841 mL	0.4205 mL	0.8410 mL	2.1025 mL
	30 mM	0.0701 mL	0.3504 mL	0.7008 mL	1.7521 mL
	40 mM	0.0526 mL	0.2628 mL	0.5256 mL	1.3141 mL
	50 mM	0.0421 mL	0.2103 mL	0.4205 mL	1.0513 mL
	60 mM	0.0350 mL	0.1752 mL	0.3504 mL	0.8760 mL
	80 mM	0.0263 mL	0.1314 mL	0.2628 mL	0.6570 mL
	100 mM	0.0210 mL	0.1051 mL	0.2103 mL	0.5256 mL

(R)-MG-132 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(R)-MG-1321211877-36-9(S,R,S)-(-)-MG-132 Z-Leu-D-Leu-Leu-alProteasomeInhibitorinhibitorinhibit

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(R)-MG-132 (Synonyms: (S,R,S)-(-)-MG-132; Z-Leu-D-Leu-Leu-al)

Customer Review

Other Forms of (R)-MG-132:

(R)-MG-132 Related Antibodies

16 Publications Citing Use of MCE (R)-MG-132

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

(R)-MG-132 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(R)-MG-132 Related Classifications

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