1. NF-κB
    MAPK/ERK Pathway
  2. NF-κB
    JNK
  3. Tomatidine

Tomatidine 

Cat. No.: HY-N2149 Purity: >98.0%
Handling Instructions

Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

For research use only. We do not sell to patients.

Tomatidine Chemical Structure

Tomatidine Chemical Structure

CAS No. : 77-59-8

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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE Tomatidine

    Tomatidine purchased from MCE. Usage Cited in: FASEB J. 2019 Feb;33(2):2574-2586.

    Tomatidine attenuates osteoclastogenesis by inhibiting the MAPK, NF-kB, and PI3K/Akt signaling pathways in vitro.

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    • Biological Activity

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    • References

    • Customer Review

    Description

    Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

    IC50 & Target[1]

    p65

     

    JNK

     

    In Vitro

    Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].

    Molecular Weight

    415.65

    Formula

    C₂₇H₄₅NO₂

    CAS No.

    77-59-8

    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 2.86 mg/mL (6.88 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4059 mL 12.0294 mL 24.0587 mL
    5 mM 0.4812 mL 2.4059 mL 4.8117 mL
    10 mM --- --- ---
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37°C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Product Name:
    Tomatidine
    Cat. No.:
    HY-N2149
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