1. NF-κB
    MAPK/ERK Pathway
  2. NF-κB


Cat. No.: HY-N2149 Purity: >98.0%
Handling Instructions

Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

For research use only. We do not sell to patients.
Tomatidine Chemical Structure

Tomatidine Chemical Structure

CAS No. : 77-59-8

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
25 mg USD 60 In-stock
50 mg USD 96 In-stock
100 mg USD 168 In-stock
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

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  • References


Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

IC50 & Target[1]





In Vitro

Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.4059 mL 12.0294 mL 24.0587 mL
5 mM 0.4812 mL 2.4059 mL 4.8117 mL
10 mM 0.2406 mL 1.2029 mL 2.4059 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Tomatidine is prepared in 0.1% DMSO or 0.1% ethanol[1].

RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37°C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







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Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: >98.0%

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