1. NF-κB
    MAPK/ERK Pathway
  2. NF-κB
    JNK

Tomatidine 

Cat. No.: HY-N2149 Purity: >98.0%
Handling Instructions

Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

For research use only. We do not sell to patients.
Tomatidine Chemical Structure

Tomatidine Chemical Structure

CAS No. : 77-59-8

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
25 mg USD 60 In-stock
50 mg USD 96 In-stock
100 mg USD 168 In-stock
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

IC50 & Target[1]

p65

 

JNK

 

In Vitro

Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.4059 mL 12.0294 mL 24.0587 mL
5 mM 0.4812 mL 2.4059 mL 4.8117 mL
10 mM 0.2406 mL 1.2029 mL 2.4059 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

Tomatidine is prepared in 0.1% DMSO or 0.1% ethanol[1].

RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37°C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

415.65

Formula

C₂₇H₄₅NO₂

CAS No.

77-59-8

SMILES

[H][[email protected]]1(O[[email protected]]2(NC[[email protected]@H](C)CC2)[[email protected]]3C)C[[email protected]@]4([H])[[email protected]]5([H])CC[[email protected]@]6([H])C[[email protected]@H](O)CC[[email protected]]6(C)[[email protected]@]5([H])CC[[email protected]]4(C)[[email protected]]13[H]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: >98.0%

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Tomatidine
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HY-N2149
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