1. NF-κB
    MAPK/ERK Pathway
    Autophagy
    Metabolic Enzyme/Protease
  2. NF-κB
    JNK
    Autophagy
    Endogenous Metabolite
  3. Tomatidine

Tomatidine 

Cat. No.: HY-N2149 Purity: ≥98.0%
COA Handling Instructions

Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling. Tomatidine activates autophagy either in mammal cells or C elegans.

For research use only. We do not sell to patients.

Tomatidine Chemical Structure

Tomatidine Chemical Structure

CAS No. : 77-59-8

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5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 70 In-stock
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25 mg USD 140 In-stock
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50 mg USD 220 In-stock
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100 mg USD 350 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Tomatidine:

Top Publications Citing Use of Products

    Tomatidine purchased from MCE. Usage Cited in: FASEB J. 2019 Feb;33(2):2574-2586.  [Abstract]

    Tomatidine attenuates osteoclastogenesis by inhibiting the MAPK, NF-kB, and PI3K/Akt signaling pathways in vitro.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Tomatidine acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling[1]. Tomatidine activates autophagy either in mammal cells or C elegans[2].

    IC50 & Target[1]

    p65

     

    JNK

     

    In Vitro

    Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    415.65

    Formula

    C27H45NO2

    CAS No.
    SMILES
    Structure Classification
    Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 16.67 mg/mL (40.11 mM; ultrasonic and warming and heat to 60°C)

    0.1 M HCL : < 1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4059 mL 12.0294 mL 24.0587 mL
    5 mM 0.4812 mL 2.4059 mL 4.8117 mL
    10 mM 0.2406 mL 1.2029 mL 2.4059 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  50% PEG300    50% saline

      Solubility: 5 mg/mL (12.03 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 0.29 mg/mL (0.70 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: ≥98.0%

    References
    Cell Assay
    [1]

    RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37°C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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    Cat. No.:
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