2. Tomatidine hydrochloride

Tomatidine hydrochloride acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling. Tomatidine hydrochloride activates autophagy either in mammal cells or C elegans.

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Tomatidine hydrochloride Chemical Structure

Tomatidine hydrochloride Chemical Structure

CAS No. : 6192-62-7

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Based on 6 publication(s) in Google Scholar

Other Forms of Tomatidine hydrochloride:

Tomatidine hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Tomatidine hydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2019 Feb;33(2):2574-2586.  [Abstract]

    Tomatidine attenuates osteoclastogenesis by inhibiting the MAPK, NF-kB, and PI3K/Akt signaling pathways in vitro.

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    Description

    Tomatidine hydrochloride acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling[1]. Tomatidine hydrochloride activates autophagy either in mammal cells or C elegans[2].

    IC50 & Target

    NF-κB[1]
    JNK[1]
    In Vitro

    Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tomatidine hydrochloride Related Antibodies
    Molecular Weight

    452.11

    Formula

    C27H46ClNO2
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    [H][C@]1(O[C@]2(NC[C@@H](C)CC2)[C@H]3C)C[C@@]4([H])[C@]5([H])CC[C@@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]13[H].Cl[H]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    Methanol : 12.5 mg/mL (27.65 mM; Need ultrasonic)

    Ethanol : 3.45 mg/mL (7.63 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2119 mL 11.0593 mL 22.1185 mL
    5 mM 0.4424 mL 2.2119 mL 4.4237 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    Purity & Documentation

    Purity: ≥98.0%

    COA (252 KB) HNMR (300 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    Cell Assay
    [1]

    RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37 °C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / Methanol 1 mM 2.2119 mL 11.0593 mL 22.1185 mL 55.2963 mL
    5 mM 0.4424 mL 2.2119 mL 4.4237 mL 11.0593 mL
    Methanol 10 mM 0.2212 mL 1.1059 mL 2.2119 mL 5.5296 mL
    15 mM 0.1475 mL 0.7373 mL 1.4746 mL 3.6864 mL
    20 mM 0.1106 mL 0.5530 mL 1.1059 mL 2.7648 mL
    25 mM 0.0885 mL 0.4424 mL 0.8847 mL 2.2119 mL
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tomatidine6192-62-7Inhibitorinhibitorinhibit

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