Tomatidine hydrochloride
Based on 10 publication(s) in Google Scholar
Tomatidine hydrochloride acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling. Tomatidine hydrochloride activates autophagy either in mammal cells or C elegans.
For research use only. We do not sell to patients.
- Purity: 99.70%
- CAS No.: 6192-62-7
- Formula: C27H46ClNO2
- Molecular Weight:452.11
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tomatidine hydrochloride
More- Drug Des Devel Ther. 2024 Dec 27:18:6313-6329. [Abstract]
- Eur J Pharmacol. 2020 Sep 5:882:173280. [Abstract]
- Sci Rep. 2026 May 8;16(1):21171. [Abstract]
- PLoS Pathog. 2025 Jan 2;21(1):e1012800. [Abstract]
- J Neurochem. 2025 May;169(5):e70087. [Abstract]
- iScience. 2023 Jul 17;26(8):107408. [Abstract]
- FASEB J. 2019 Feb;33(2):2574-2586. [Abstract]
- Res Sq. 2025 Jul 13.
- Research Square Preprint. 2023 Apr 27.
- Aging (Albany NY). 2020 Jul 5;12(13):12799-12811. [Abstract]
Customer Validation & Images
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All Endogenous Metabolite Isoforms
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Biological Activity
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p65 |
JNK |
Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6192-62-7
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Appearance Solid
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Molecular Weight 452.11
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Formula C27H46ClNO2
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Color White to yellow
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SMILES
[H][C@]1(O[C@]2(NC[C@@H](C)CC2)[C@H]3C)C[C@@]4([H])[C@]5([H])CC[C@@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]13[H].Cl[H]
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (10)
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Journal Impact Factor
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Most Recent
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Drug Des Devel Ther
Tomatidine Alleviates Intervertebral Disc Degeneration by Activating the Nrf2/HO-1/GPX4 Signaling Pathway. [Abstract]2024 Dec 27:18:6313-6329. PMID: 39741916 -
Eur J Pharmacol
2020 Sep 5:882:173280. PMID: 32580039 -
Sci Rep
Tomatidine modulatesfibrosis-related markers by promoting autophagy in hepatic stellate cells via the ERK/MAPK-mTOR-ULK1 signaling pathway. [Abstract]2026 May 8;16(1):21171. PMID: 42103827 -
PLoS Pathog
2025 Jan 2;21(1):e1012800. PMID: 39746094 -
J Neurochem
Tomatidine Ameliorates Diabetes-Induced Cognitive Impairment and Tau Hyperphosphorylation Through the AMPK-TFEB Pathway. [Abstract]2025 May;169(5):e70087. PMID: 40387453 -
iScience
Tomatidine targets ATF4-dependent signaling and induces ferroptosis to limit pancreatic cancer progression. [Abstract]2023 Jul 17;26(8):107408. PMID: 37554459 -
FASEB J
Tomatidine suppresses osteoclastogenesis and mitigates estrogen deficiency-induced bone mass loss by modulating TRAF6-mediated signaling. [Abstract]2019 Feb;33(2):2574-2586. PMID: 30285579
Tomatidine hydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2019 Feb;33(2):2574-2586. [Abstract]
Tomatidine attenuates osteoclastogenesis by inhibiting the MAPK, NF-kB, and PI3K/Akt signaling pathways in vitro.
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Aging (Albany NY)
Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats. [Abstract]2020 Jul 5;12(13):12799-12811. PMID: 32628132
Solvent & Solubility
Methanol : 12.5 mg/mL (27.65 mM; Need ultrasonic)
Ethanol : 3.33 mg/mL (7.37 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37 °C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chiu FL, et al. Tomatidine inhibits iNOS and COX-2 through suppression of NF-kappaB and JNK pathways in LPS-stimulated mouse macrophages. FEBS Lett. 2008 Jul 9;582(16):2407-12. [Content Brief]
[2]. Anil Ahsan, et al. Tomatidine Protects Against Ischemic Neuronal Injury by Improving Lysosomal Function. Eur J Pharmacol. 2020 Jun 21;173280. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| Ethanol / Methanol | 1 mM | 2.2119 mL | 11.0593 mL | 22.1185 mL | 55.2963 mL |
| 5 mM | 0.4424 mL | 2.2119 mL | 4.4237 mL | 11.0593 mL | |
| Methanol | 10 mM | 0.2212 mL | 1.1059 mL | 2.2119 mL | 5.5296 mL |
| 15 mM | 0.1475 mL | 0.7373 mL | 1.4746 mL | 3.6864 mL | |
| 20 mM | 0.1106 mL | 0.5530 mL | 1.1059 mL | 2.7648 mL | |
| 25 mM | 0.0885 mL | 0.4424 mL | 0.8847 mL | 2.2119 mL |
Tomatidine hydrochloride Related Classifications
HY-N2149A Related Classifications
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