1. NF-κB
    MAPK/ERK Pathway
  2. NF-κB
    JNK
  3. Tomatidine hydrochloride

Tomatidine hydrochloride 

Cat. No.: HY-N2149A
Handling Instructions

Tomatidine hydrochloride acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

For research use only. We do not sell to patients.

Tomatidine hydrochloride Chemical Structure

Tomatidine hydrochloride Chemical Structure

CAS No. : 6192-62-7

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Top Publications Citing Use of Products

Publications Citing Use of MCE Tomatidine hydrochloride

    Tomatidine hydrochloride purchased from MCE. Usage Cited in: FASEB J. 2019 Feb;33(2):2574-2586.

    Tomatidine attenuates osteoclastogenesis by inhibiting the MAPK, NF-kB, and PI3K/Akt signaling pathways in vitro.

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    Description

    Tomatidine hydrochloride acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling.

    IC50 & Target

    p65

     

    JNK

     

    In Vitro

    Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity[1].

    Molecular Weight

    452.11

    Formula

    C₂₇H₄₆ClNO₂

    CAS No.

    6192-62-7

    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.

    References
    Cell Assay
    [1]

    RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO2 atmosphere at 37 °C in a humidified incubator. For all assay, cell is plated at 2×105 cells/cm2 in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    TomatidineNF-κBJNKNuclear factor-κBNuclear factor-kappaBc-Jun N-terminal kinaseanti-inflammatorychemopreventionLPS-stimulatedmousemacrophagesphosphorylationInhibitorinhibitorinhibit

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    Cat. No.:
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