Artemisinin (Synonyms: Qinghaosu; NSC 369397)

Name: Artemisinin
Brand: MedChemExpress
SKU: HY-B0094
Rating: 5.0 (32 reviews)

Cat. No.: HY-B0094 Purity: 98.0%: Data Sheet
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Artemisinin (Qinghaosu), a sesquiterpene lactone, is an anti-malarial agent isolated from the aerial parts of Artemisia annua L. plants. Artemisinin inhibits AKT signaling pathway by decreasing pAKT in a dose-dependent manner. Artemisinin reduces cancer cell proliferation, migration, invasion, tumorigenesis and metastasis and has neuroprotective effects.

For research use only. We do not sell to patients.

CAS No. : 63968-64-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 32 publication(s) in Google Scholar

Other Forms of Artemisinin:

32 Publications Citing Use of MCE Artemisinin

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

In Vivo Imaging

: Artemisinin purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117. [Abstract]; ART (50 μM, 15 min) pre-incubation significantly inhibited the Yoda1-induced increase in calcium ion influx in human umbilical vein endothelial cells (HUVECs).

: Artemisinin purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117. [Abstract]; Yoda1 greatly increased the calcium influx in OA HACs, which was dramatically reduced by addition of ART (50 μM, 15 min).

: Artemisinin purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117. [Abstract]; CCK8 assay was used to detect the effects of different concentrations of ART (0-1000 μM) on OA HACs proliferation. The black trace represents the average response of 3-dish repeats. Error bars are shown in colors.

: Artemisinin purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117. [Abstract]; KEGG pathway analysis of transcripts from experiments of Yoda1 and Yoda1 + ART. The three biologically independent samples in each group were combined into one.

: Artemisinin purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117. [Abstract]; Western blot of PIEZO1, COL2A1, RUNX2 and MMP13 in OA HACs treated with Yoda1 with and without ART (50 μM) for 24 h.

: Artemisinin purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117. [Abstract]; Representative 3D reconstruction derived from μCT scans of knee joints from Yoda1-treated mice and controls with or without ART (1 mM, 2 μL/time, intra-articular administration, once a week) treatment at 8 weeks after DMM surgery. Scale bar, 1.0 mm.

: Artemisinin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Jan;20(1):51-64. [Abstract]; Comparison of drug (Epirubicin hydrochloride, EPI; Daunorubicin hydrochloride, DNR; Vinorelbine ditartrate, VNR; Oxaliplatin, OXA; Vincristine, VCR; Artemisinin, ART; Colchicine, COL) cytotoxicity to TC1 cells at the indicated doses and time points measured with CCK-8 assays. R.U. (Relative unit) was calculated from the average O.D. values in each condition as the indicator of cell viability (n = 3).

: Artemisinin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Oct;118:109383. [Abstract]; ART (Artemisinin) suppresses the phosphorylation of AKT in UMRC-2 xenograft tumor. Tumors are homogenized in RIPA buffer. The protein levels were measured by western blot analysis.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Artemisinin (Qinghaosu), a sesquiterpene lactone, is an anti-malarial agent isolated from the aerial parts of Artemisia annua L. plants^[1]. Artemisinin inhibits AKT signaling pathway by decreasing pAKT in a dose-dependent manner. Artemisinin reduces cancer cell proliferation, migration, invasion, tumorigenesis and metastasis and has neuroprotective effects^[2].

IC₅₀ & Target^[2]

Plasmodium

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human A375 cells after 72 hrs by CCK-8 assay Antiproliferative activity against human A375 cells after 72 hrs by CCK-8 assay	[PMID: 29597166]
A-375	IC₅₀	> 50 μM Compound: Artemisinin	Cytotoxicity against human A375 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A375 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29789258]
A-431	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human A431 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human A431 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31546197]
A-431	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human A-431 cells Antiproliferative activity against human A-431 cells	[PMID: 31945642]
A549	IC₅₀	15.2 μM Compound: 1a	Anticancer activity against human A549 cells by sulforhodamine B assay Anticancer activity against human A549 cells by sulforhodamine B assay	[PMID: 19819696]
A549	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31546197]
A549	IC₅₀	9.85 μM Compound: Artemisinin	Anticancer activity against human A549 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Anticancer activity against human A549 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 34303874]
A549	IC₅₀	> 20 μM Compound: Artemisinin	Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by SRB assay Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by SRB assay	[PMID: 26318055]
A549	IC₅₀	> 300 μM Compound: Artemisinin	Antiproliferative activity against human A549 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition by MTT assay	[PMID: 31945642]
A549	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human A549 cells after 72 hrs by CCK-8 assay Antiproliferative activity against human A549 cells after 72 hrs by CCK-8 assay	[PMID: 29597166]
A549	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human A549 cells measured after 48 hrs by MTT assay Antiproliferative activity against human A549 cells measured after 48 hrs by MTT assay	[PMID: 30837097]
A549	IC₅₀	> 50 μM Compound: Artemisinin	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29789258]
A549	IC₅₀	> 50 μM Compound: Artemisinin	Antiproliferative activity against human A549 cells Antiproliferative activity against human A549 cells	[PMID: 31945642]
BEAS-2B	IC₅₀	7.53 μM Compound: Artemisinin	Anticancer activity against human BEAS-2B cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Anticancer activity against human BEAS-2B cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 34303874]
BT-549	IC₅₀	40 μM Compound: Artemisinin	Antiproliferative activity against human BT-549 cells assessed as cell growth inhibition measured after 72 hrs by SRB assay Antiproliferative activity against human BT-549 cells assessed as cell growth inhibition measured after 72 hrs by SRB assay	[PMID: 27010926]
Breast cancer cell line	IC₅₀	>= 40 μM Compound: Artemisinin	Antiproliferative activity against human Breast cancer cell line Antiproliferative activity against human Breast cancer cell line	[PMID: 31945642]
CAKI-2	IC₅₀	40 μM Compound: Artemisinin	Antiproliferative activity against human CAKI-2 cells assessed as inhibition of cell growth Antiproliferative activity against human CAKI-2 cells assessed as inhibition of cell growth	[PMID: 27010926]
CCRF-CEM	EC₅₀	36.9 μM Compound: 1	Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 29887512]
CCRF-CEM	IC₅₀	36.9 μM Compound: 1	Cytotoxicity against human CCRF-CEM cells by resazurin assay Cytotoxicity against human CCRF-CEM cells by resazurin assay	[PMID: 24561670]
CCRF-CEM	IC₅₀	36.9 μM Compound: 1	Cytotoxicity against human CCRF-CEM cells assessed as cell viability after 72 hrs by resazurin assay Cytotoxicity against human CCRF-CEM cells assessed as cell viability after 72 hrs by resazurin assay	[PMID: 26260339]
CCRF-CEM	IC₅₀	36.9 μM Compound: Artemisinin	Cytotoxicity against human CCRF-CEM cells by CCK-8 assay Cytotoxicity against human CCRF-CEM cells by CCK-8 assay	[PMID: 22884578]
CCRF-CEM	IC₅₀	36.9 μM Compound: artemisinin	Cytotoxicity against human CCRF-CEM by XTT assay Cytotoxicity against human CCRF-CEM by XTT assay	[PMID: 20527917]
CFU-GM	IC₅₀	385 μM Compound: 165	Cytotoxicity against human CFUGM Cytotoxicity against human CFUGM	[PMID: 17618015]
COLO 205	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human COLO205 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human COLO205 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31546197]
COLO 205	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human COLO 205 cells Antiproliferative activity against human COLO 205 cells	[PMID: 31945642]
Ehrlich	IC₅₀	29.8 μM Compound: 1	Cytotoxicity against mouse EAC after 3 days by MTT assay Cytotoxicity against mouse EAC after 3 days by MTT assay	[PMID: 8350087]
FM3A	EC₅₀	10 nM Compound: artemisinin	Antiproliferative activity in Mouse mammary tumor FM3A Antiproliferative activity in Mouse mammary tumor FM3A	[PMID: 10966746]
FM3A	EC₅₀	10 μM Compound: Artemisinin	Mammalian cell cytotoxicity assessed against mouse mammary tumor FM3A cells Mammalian cell cytotoxicity assessed against mouse mammary tumor FM3A cells	[PMID: 11882006]
FM3A	EC₅₀	10 μM Compound: artemisinin	In vitro cytotoxicity in mouse mammary tumor FM3A cells In vitro cytotoxicity in mouse mammary tumor FM3A cells	[PMID: 10411480]
FM3A	EC₅₀	10 μM Compound: artemisinin	Effective concentration against mouse mammary FM3A cells Effective concentration against mouse mammary FM3A cells	[PMID: 12361400]
FM3A	EC₅₀	10 μM Compound: artemisinin	Compound was tested in vitro cytotoxicity against FM3A cell Compound was tested in vitro cytotoxicity against FM3A cell	[PMID: 12723958]
FM3A	EC₅₀	1.0 x 10^-5 M Compound: Artemisinin	In vitro cytotoxicity against mouse mammary FM3A cells In vitro cytotoxicity against mouse mammary FM3A cells	[PMID: 12036365]
FM3A	EC₅₀	1 x 10^-5 M Compound: artemisinin	Compound was tested for cytotoxicity against FM3A mouse mammary cells. Compound was tested for cytotoxicity against FM3A mouse mammary cells.	[PMID: 10447961]
FM3A	EC₅₀	1.0 x 10^-5 M Compound: artemisinin	in vitro cytotoxicity against mouse mammary tumor in cell culture. in vitro cytotoxicity against mouse mammary tumor in cell culture.	[PMID: 11428929]
FM3A	EC₅₀	1.0 x 10^-5 M Compound: artemisinin	Cytotoxicity against FM3A mouse mammary cells Cytotoxicity against FM3A mouse mammary cells	[PMID: 13678413]
FM3A	IC₅₀	5.0 x 10^-9 M Compound: 1	Mean of EC50 values for FM3A cells/ mean of EC50 values for Plasmodium falciparum Mean of EC50 values for FM3A cells/ mean of EC50 values for Plasmodium falciparum	[PMID: 9057849]
HCT-116	GI₅₀	22.5 μM Compound: ART	Growth inhibition of human HCT-116 cells incubated for 24 hrs by Cell-titer Glo luminescent assay Growth inhibition of human HCT-116 cells incubated for 24 hrs by Cell-titer Glo luminescent assay	[PMID: 38287228]
HCT-116	GI₅₀	4.3 μM Compound: ART	Growth inhibition of human HCT-116 cells incubated for 72 hrs by Cell-titer Glo luminescent assay Growth inhibition of human HCT-116 cells incubated for 72 hrs by Cell-titer Glo luminescent assay	[PMID: 38287228]
HCT-116	GI₅₀	7.2 μM Compound: ART	Growth inhibition of human HCT-116 cells incubated for 48 hrs by Cell-titer Glo luminescent assay Growth inhibition of human HCT-116 cells incubated for 48 hrs by Cell-titer Glo luminescent assay	[PMID: 38287228]
HCT-116	IC₅₀	7.2 μM Compound: ART	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth by Cell-titer Glo luminescent assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth by Cell-titer Glo luminescent assay	[PMID: 38287228]
HCT-116	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human HCT116 cells after 72 hrs by CCK-8 assay Antiproliferative activity against human HCT116 cells after 72 hrs by CCK-8 assay	[PMID: 29597166]
HCT-15	IC₅₀	17.2 μM Compound: 1a	Anticancer activity against human HCT15 cells by sulforhodamine B assay Anticancer activity against human HCT15 cells by sulforhodamine B assay	[PMID: 19819696]
HCT-15	IC₅₀	> 300 μM Compound: Artemisinin	Antiproliferative activity against human HCT-15 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human HCT-15 cells assessed as cell growth inhibition by MTT assay	[PMID: 31945642]
HEK293	CC₅₀	> 26 μM Compound: Artemisinin	Cytotoxicity against human HEK293 cells assessed as cell viability measured for 24 hrs by MTT assay Cytotoxicity against human HEK293 cells assessed as cell viability measured for 24 hrs by MTT assay	[PMID: 34303874]
HEK293	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human HEK293 cells Antiproliferative activity against human HEK293 cells	[PMID: 31945642]
HEK293	IC₅₀	> 120 nM Compound: Artemisinin	Cytotoxicity against HEK293 cells by alamar blue assay Cytotoxicity against HEK293 cells by alamar blue assay	[PMID: 27212070]
HFF	EC₅₀	0.8 μM Compound: Artemisinin	Antiparasitic activity against Toxoplasma gondii ATCC 50839 infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay Antiparasitic activity against Toxoplasma gondii ATCC 50839 infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay	[PMID: 17698618]
HFF	EC₅₀	1.51 μM Compound: Artemisinin	Antiparasitic activity against artemisinin-resistant Toxoplasma gondii KN200-6 infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay Antiparasitic activity against artemisinin-resistant Toxoplasma gondii KN200-6 infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay	[PMID: 17698618]
HFF	EC₅₀	1.73 μM Compound: Artemisinin	Antiparasitic activity against artemisinin-resistant Toxoplasma gondii KN200-1 infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay Antiparasitic activity against artemisinin-resistant Toxoplasma gondii KN200-1 infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay	[PMID: 17698618]
HFF	EC₅₀	1.8 μM Compound: Artemisinin	Antiparasitic activity against artemisinin-resistant Toxoplasma gondii STL500-10A infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay Antiparasitic activity against artemisinin-resistant Toxoplasma gondii STL500-10A infected in HFF cells after 72 hrs by beta-galactosidase reporter gene assay	[PMID: 17698618]
HFF	IC₅₀	0.64 μM Compound: Artemisinin	Antiparasitic activity against Toxoplasma gondii 2F infected in HFF cells assessed as beta galactosidase activity after 5 days Antiparasitic activity against Toxoplasma gondii 2F infected in HFF cells assessed as beta galactosidase activity after 5 days	[PMID: 20373807]
HL-60	IC₅₀	40 μM Compound: Artemisinin	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition measured after 48 hrs by SRB assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition measured after 48 hrs by SRB assay	[PMID: 27010926]
HL-60	IC₅₀	> 50 μM Compound: 3	Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry	[PMID: 22985027]
HT-29	ED₅₀	1.25 μg/mL Compound: Artemisinin	Cytotoxicity against human HT-29 cells Cytotoxicity against human HT-29 cells	[PMID: 31784199]
HUVEC	IC₅₀	1.1 μM Compound: ART	Cytotoxicity against HUVEC assessed as reduction in cell number after 48 hrs by MTT assay Cytotoxicity against HUVEC assessed as reduction in cell number after 48 hrs by MTT assay	[PMID: 28549888]
HUVEC	IC₅₀	> 50 μM Compound: 1	Inhibitory activity against human umbilical vein endothelial cells (HUVEC) was assayed using MTT colorimetric proliferation assay Inhibitory activity against human umbilical vein endothelial cells (HUVEC) was assayed using MTT colorimetric proliferation assay	[PMID: 14552753]
HeLa	IC₅₀	493.38 μM Compound: ARS	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth	[PMID: 36538859]
HeLa	IC₅₀	493.38 μM Compound: Artemisinin	Cytotoxicity against human HeLa cells measured at 48 hrs by MTT assay Cytotoxicity against human HeLa cells measured at 48 hrs by MTT assay	[PMID: 26099535]
HepG2	CC₅₀	> 10 μM Compound: Artemisinin	Cytotoxicity against human HepG2 cells expressing CD81 assessed as cell viability after 72 hrs by CellTiter-glo assay Cytotoxicity against human HepG2 cells expressing CD81 assessed as cell viability after 72 hrs by CellTiter-glo assay	[PMID: 25791675]
HepG2	CC₅₀	> 30 μM Compound: ART	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by CellTiter-Glo assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by CellTiter-Glo assay	[PMID: 33620219]
HepG2	IC₅₀	12 μM Compound: ART	Antimalarial activity against sporozoite stage of Plasmodium berghei yoelii infected in human HepG2 cells Antimalarial activity against sporozoite stage of Plasmodium berghei yoelii infected in human HepG2 cells	[PMID: 26640981]
HepG2	IC₅₀	4.09 μM Compound: Artemisinin	Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 34303874]
HepG2	IC₅₀	97 μM Compound: ARS	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay	[PMID: 36538859]
HepG2	IC₅₀	> 10000 nM Compound: Artemisinin	Antiplasmodial activity against liver stage of Plasmodium yoelii 17X NL sporozoites infected in human HepG2 cells expressing CD81 after 48 hrs by DAPI staining-based immunofluorescence analysis Antiplasmodial activity against liver stage of Plasmodium yoelii 17X NL sporozoites infected in human HepG2 cells expressing CD81 after 48 hrs by DAPI staining-based immunofluorescence analysis	[PMID: 25791675]
HepG2	IC₅₀	> 200 μM Compound: ART	Cytotoxicity against human HepG2 cells after 72 hrs by formazan test Cytotoxicity against human HepG2 cells after 72 hrs by formazan test	[PMID: 23685181]
HepG2	IC₅₀	> 300 μM Compound: Artemisinin	Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition by MTT assay	[PMID: 31945642]
HepG2	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human HepG2 cells measured after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells measured after 48 hrs by MTT assay	[PMID: 30837097]
HepG2 2.2.15	IC₅₀	55 μM Compound: 20	Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV surface antigen secretion after 21 days Antiviral activity against Hepatitis B virus infected in human HepG2.2.15 cells assessed as decrease in HBV surface antigen secretion after 21 days	[PMID: 24549242]
Hepatocyte	EC₅₀	0.6 μM Compound: QHS, Qing-hao-su	Induction of CYP2B6 in human hepatocytes after 72 hrs relative to vehicle-treated control Induction of CYP2B6 in human hepatocytes after 72 hrs relative to vehicle-treated control	[PMID: 22679214]
Hepatocyte	EC₅₀	4 μM Compound: QHS, Qing-hao-su	Induction of CYP2A6 mRNA expression in human hepatocytes after 72 hrs by RT-PCR analysis relative to vehicle-treated control Induction of CYP2A6 mRNA expression in human hepatocytes after 72 hrs by RT-PCR analysis relative to vehicle-treated control	[PMID: 22679214]
Hepatocyte	EC₅₀	5.2 μM Compound: QHS, Qing-hao-su	Induction of CYP1A2 mRNA expression in human hepatocytes after 72 hrs by RT-PCR analysis relative to vehicle-treated control Induction of CYP1A2 mRNA expression in human hepatocytes after 72 hrs by RT-PCR analysis relative to vehicle-treated control	[PMID: 22679214]
Hepatocyte	EC₅₀	5.9 μM Compound: QHS, Qing-hao-su	Induction of CYP3A4 in human hepatocytes after 72 hrs relative to vehicle-treated control Induction of CYP3A4 in human hepatocytes after 72 hrs relative to vehicle-treated control	[PMID: 22679214]
Huh-7	CC₅₀	> 10 μM Compound: GNF-Pf-5341	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]
Huh-7	CC₅₀	> 100 μg/mL Compound: Artemisinin	Cytotoxicity against human HuH7 cells after 72 hrs by MTT assay Cytotoxicity against human HuH7 cells after 72 hrs by MTT assay	[PMID: 26922227]
Huh-7	IC₅₀	> 10 μM Compound: 1, ART	Antimalarial activity against liver stage of Plasmodium berghei infected in human Huh7 cells after 48 hrs by luciferase assay Antimalarial activity against liver stage of Plasmodium berghei infected in human Huh7 cells after 48 hrs by luciferase assay	[PMID: 24900781]
K562	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31546197]
K562	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human K562 cells Antiproliferative activity against human K562 cells	[PMID: 31945642]
KB	ED₅₀	1.25 μg/mL Compound: Artemisinin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 31784199]
KB	ED₅₀	> 20 μg/mL Compound: artemisinin	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 11421741]
KB	ED₅₀	> 20 μg/mL Compound: artemisinin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 16933872]
KB	ED₅₀	> 70900 nM Compound: artemisinin	Cytotoxicity against human KB cells after 72 hrs by SRB assay Cytotoxicity against human KB cells after 72 hrs by SRB assay	[PMID: 9917283]
KB	IC₅₀	16 μg/mL Compound: 8	Compound was tested for in vitro cytotoxicity against KB (human epidermoid carcinoma) cell line Compound was tested for in vitro cytotoxicity against KB (human epidermoid carcinoma) cell line	10.1016/S0960-894X(97)00169-8
KB	IC₅₀	> 20 μM Compound: Artemisinin	Cytotoxicity against human KB cells assessed as inhibition of cell proliferation after 72 hrs by SRB assay Cytotoxicity against human KB cells assessed as inhibition of cell proliferation after 72 hrs by SRB assay	[PMID: 26318055]
KB	IC₅₀	> 20000 ng/mL Compound: Artemisinin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 8759172]
KB	IC₅₀	> 20000 ng/mL Compound: artemisinin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 1294700]
KB	IC₅₀	> 20000 ng/mL Compound: artemisinin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 9134742]
KB	IC₅₀	> 354 μM Compound: artemisinin	Cytotoxicity against human KB cells after 72 hrs by alamar blue assay Cytotoxicity against human KB cells after 72 hrs by alamar blue assay	[PMID: 19957999]
L02	IC₅₀	0.43 μM Compound: 1b; ARS	Cytotoxicity against human L02 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human L02 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 33636536]
L02	IC₅₀	8.25 μM Compound: Artemisinin	Anticancer activity against human L02 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Anticancer activity against human L02 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 34303874]
L02	IC₅₀	> 100 μM Compound: ART	Cytotoxicity against human L02 cells measured after 48 hrs by MTT assay Cytotoxicity against human L02 cells measured after 48 hrs by MTT assay	[PMID: 30837097]
L02	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human L02 cells after 72 hrs by CCK-8 assay Antiproliferative activity against human L02 cells after 72 hrs by CCK-8 assay	[PMID: 29597166]
L02	IC₅₀	> 50 μM Compound: Artemisinin	Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29789258]
L6	CC₅₀	> 350 μM Compound: ART	Cytotoxicity against rat L6 cels assessed as reduction in cell viability after 72 hrs by Alamar Blue dye-based fluorometric analysis Cytotoxicity against rat L6 cels assessed as reduction in cell viability after 72 hrs by Alamar Blue dye-based fluorometric analysis	[PMID: 30615444]
L6	IC₅₀	150 μM Compound: ATM	Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay	[PMID: 23795673]
L6	IC₅₀	170 μM Compound: artemisinin	Cytotoxicity against rat L6 cells Cytotoxicity against rat L6 cells	[PMID: 19091562]
L6	IC₅₀	450 μM Compound: ART	Cytotoxicity against rat L6 cells after 70 hrs by alamar blue staining based fluorometric assay Cytotoxicity against rat L6 cells after 70 hrs by alamar blue staining based fluorometric assay	[PMID: 28279559]
L6	IC₅₀	450 μM Compound: ATM	Cytotoxicity against rat L6 cells after 70 hrs by alamar blue assay Cytotoxicity against rat L6 cells after 70 hrs by alamar blue assay	[PMID: 19267462]
L6	IC₅₀	450 μM Compound: ATMS	Cytotoxicity against rat L6 cells Cytotoxicity against rat L6 cells	[PMID: 17948982]
L6	IC₅₀	450 μM Compound: ATMS	Cytotoxicity against rat L6 cells Cytotoxicity against rat L6 cells	[PMID: 18841956]
L6	IC₅₀	450 μM Compound: ATMS	Cytotoxicity against rat L6 cells after 70 hrs by alamar blue assay Cytotoxicity against rat L6 cells after 70 hrs by alamar blue assay	[PMID: 19606902]
L6	IC₅₀	450 μM Compound: ATMS	Cytotoxicity against rat L6 cells after 70 hrs by alamar blue assay Cytotoxicity against rat L6 cells after 70 hrs by alamar blue assay	[PMID: 19928900]
L6	IC₅₀	450.5 μM Compound: ART	Cytotoxicity against rat L6 cells assessed as reduction in cell viability after 72 hrs by Alamar blue assay Cytotoxicity against rat L6 cells assessed as reduction in cell viability after 72 hrs by Alamar blue assay	[PMID: 27344215]
L6	IC₅₀	450.5 μM Compound: ART	Cytotoxicity in rat L6 cells assessed as reduction in cell viability incubated for 72 hrs by alamar blue assay Cytotoxicity in rat L6 cells assessed as reduction in cell viability incubated for 72 hrs by alamar blue assay	[PMID: 30962114]
L6	IC₅₀	450.5 μM Compound: ATMS	Cytotoxicity against rat L6 cells Cytotoxicity against rat L6 cells	[PMID: 19409677]
L6	IC₅₀	450.5 μM Compound: Art	Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay	[PMID: 19395265]
L6	IC₅₀	450.5 μM Compound: Art	Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorometry Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorometry	[PMID: 23880082]
L6	IC₅₀	450.5 μM Compound: Artemisinin	Cytotoxicity against rat L6 cells Cytotoxicity against rat L6 cells	[PMID: 19879671]
L6	IC₅₀	450.5 μM Compound: At	Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay	[PMID: 25746816]
L6	IC₅₀	450.5 μM Compound: art	Cytotoxicity against L6 cells Cytotoxicity against L6 cells	[PMID: 16889962]
L6	IC₅₀	450.5 μM Compound: art	Cytotoxicity against rat L6 cells after 72 hrs by microplate assay Cytotoxicity against rat L6 cells after 72 hrs by microplate assay	[PMID: 17544672]
L6	IC₅₀	450.5 μM Compound: art	Cytotoxicity against rat L6 cells Cytotoxicity against rat L6 cells	[PMID: 20709557]
L6	IC₅₀	450.5 μM Compound: art	Cytotoxicity against rat L6 cells Cytotoxicity against rat L6 cells	[PMID: 22136906]
L6	IC₅₀	450.5 μM Compound: art	Cytotoxicity against rat L6 cells after 70 hrs by Alamar blue assay Cytotoxicity against rat L6 cells after 70 hrs by Alamar blue assay	[PMID: 33148495]
L6	IC₅₀	450.5 μM Compound: artemisinin (art)	Cytotoxicity against rat L6 cells after 72 hrs by microplate alamar blue assay Cytotoxicity against rat L6 cells after 72 hrs by microplate alamar blue assay	[PMID: 18502136]
L6	IC₅₀	> 180 μM Compound: ATM	Cytotoxicity against rat L6 cells assessed as cell viability after 70 hrs by Alamar blue assay Cytotoxicity against rat L6 cells assessed as cell viability after 70 hrs by Alamar blue assay	[PMID: 24268543]
L6	IC₅₀	> 319 mM Compound: ATMS	Cytotoxicity against rat L6 cells after 72 hrs by Alamar blue assay Cytotoxicity against rat L6 cells after 72 hrs by Alamar blue assay	[PMID: 23871911]
L929	EC₅₀	> 100 μg/mL Compound: artemisinin	Cytotoxicity against mouse L929 cells by MTT assay Cytotoxicity against mouse L929 cells by MTT assay	[PMID: 16854076]
L929	IC₅₀	> 40 μg/mL Compound: Artemisinine	Cytotoxicity against mouse L929 cells after 5 days by MTT assay Cytotoxicity against mouse L929 cells after 5 days by MTT assay	[PMID: 18922036]
LLC-PK1	IC₅₀	0.6 μg/mL Compound: artemisinin	Cytotoxicity against pig LLC-PK1 cells after 48 hrs by neutral red assay Cytotoxicity against pig LLC-PK1 cells after 48 hrs by neutral red assay	[PMID: 19105653]
LS174T	EC₅₀	30 μM Compound: QHS, Qing-hao-su	Activation of human PXR expressed in human LS174T cells Activation of human PXR expressed in human LS174T cells	[PMID: 22679214]
LS174T	IC₅₀	> 200 μM Compound: ART	Cytotoxicity against human LS 174T cells after 72 hrs by formazan test Cytotoxicity against human LS 174T cells after 72 hrs by formazan test	[PMID: 23685181]
MCF7	ED₅₀	1.25 μg/mL Compound: Artemisinin	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 31784199]
MCF7	IC₅₀	1.4 x 10^-4 μM Compound: ART	Cytotoxicity against human MCF7 cells assessed as reduction in cell number after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell number after 48 hrs by MTT assay	[PMID: 28549888]
MCF7	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human MCF7 cells measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells measured after 48 hrs by MTT assay	[PMID: 30837097]
MCF7	IC₅₀	> 50 μM Compound: Artemisinin	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29789258]
MCF7	IC₅₀	> 50 μM Compound: Artemisinin	Anticancer activity against human MCF7 cells Anticancer activity against human MCF7 cells	[PMID: 31945642]
MDA-MB-231	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31546197]
MDA-MB-231	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human MDA-MB-231 cells Antiproliferative activity against human MDA-MB-231 cells	[PMID: 31945642]
MDA-MB-231	IC₅₀	> 100 μM Compound: ART	Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by MTT assay	[PMID: 30837097]
MDA-MB-231	IC₅₀	> 50 μM Compound: Artemisinin	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29789258]
MDA-MB-231	IC₅₀	> 50 μM Compound: Artemisinin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability	[PMID: 31945642]
MRC5	CC₅₀	> 25 μM Compound: Artemisinin	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 36282784]
MRC5	IC₅₀	50.3 μM Compound: Artemisinin	Cytotoxicity against human MRC5 cells assessed as cell growth inhibition and measured after 2 days by WST-8 assay Cytotoxicity against human MRC5 cells assessed as cell growth inhibition and measured after 2 days by WST-8 assay	[PMID: 35512262]
MRC5	IC₅₀	> 25 μM Compound: Artemisinin	Cytotoxicity against human MRC5 cells assessed as inhibition of cell growth Cytotoxicity against human MRC5 cells assessed as inhibition of cell growth	[PMID: 38421618]
Melanoma cell line	IC₅₀	>= 40 μM Compound: Artemisinin	Antiproliferative activity against human Melanoma cell line Antiproliferative activity against human Melanoma cell line	[PMID: 31945642]
Ovarian cancer cell line	IC₅₀	>= 40 μM Compound: Artemisinin	Antiproliferative activity against human Ovarian cancer cell line Antiproliferative activity against human Ovarian cancer cell line	[PMID: 31945642]
P388	ED₅₀	1.25 μg/mL Compound: Artemisinin	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 31784199]
P388	IC₅₀	28 μg/mL Compound: 8	Compound was tested for in vitro cytotoxicity against P388 (murine lymphocytic leukemia) cell line Compound was tested for in vitro cytotoxicity against P388 (murine lymphocytic leukemia) cell line	10.1016/S0960-894X(97)00169-8
PC-12	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against rat PC12 cells after 72 hrs by CCK-8 assay Antiproliferative activity against rat PC12 cells after 72 hrs by CCK-8 assay	[PMID: 29597166]
PC-3	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31546197]
PC-3	IC₅₀	39.03 μM Compound: Artemisinin	Antiproliferative activity against human PC-3 cells Antiproliferative activity against human PC-3 cells	[PMID: 31945642]
SH-SY5Y	IC₅₀	> 50 μM Compound: ART	Antiproliferative activity against human SH-SY5Y cells after 72 hrs by CCK-8 assay Antiproliferative activity against human SH-SY5Y cells after 72 hrs by CCK-8 assay	[PMID: 29597166]
SH-SY5Y	IC₅₀	> 50 μM Compound: Artemisinin	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29789258]
SK-HEP1	IC₅₀	> 200 μM Compound: ART	Cytotoxicity against human SKHEP1 cells after 72 hrs by formazan test Cytotoxicity against human SKHEP1 cells after 72 hrs by formazan test	[PMID: 23685181]
SK-MEL-2	IC₅₀	12.9 μM Compound: 1a	Anticancer activity against human SK-MEL-2 cells by sulforhodamine B assay Anticancer activity against human SK-MEL-2 cells by sulforhodamine B assay	[PMID: 19819696]
SK-OV-3	IC₅₀	14.3 μM Compound: 1a	Anticancer activity against human SKOV3 cells by sulforhodamine B assay Anticancer activity against human SKOV3 cells by sulforhodamine B assay	[PMID: 19819696]
SMMC-7721	IC₅₀	0.44 μM Compound: Artemisinin	Antiproliferative activity against human SMMC-7721 cells Antiproliferative activity against human SMMC-7721 cells	[PMID: 31945642]
SMMC-7721	IC₅₀	0.63 μM Compound: 1b; ARS	Cytotoxicity against human SMMC-7721 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human SMMC-7721 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 33636536]
T-cell	IC₅₀	4.43 x 10^-6 M Compound: Artemisinin	In vitro concentration of compound required to inhibit ConA-induced T cell proliferation to 50% in BALB/c mice In vitro concentration of compound required to inhibit ConA-induced T cell proliferation to 50% in BALB/c mice	[PMID: 15999998]
U-87MG ATCC	IC₅₀	> 50 μM Compound: Artemisinin	Cytotoxicity against human U87 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human U87 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29789258]
Vero	CC₅₀	130 μM Compound: Artemisinin	Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell proliferation Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell proliferation	[PMID: 33636304]
Vero	CC₅₀	434.6 μM Compound: ART	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 37163950]
Vero	IC₅₀	28 nM Compound: Artemisinin	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in African green monkey (Cercopithecus aethiops) (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in African green monkey (Cercopithecus aethiops) (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs	[PMID: 19833520]
Vero	IC₅₀	31 μg/mL Compound: 8	Compound was tested for in vitro cytotoxicity against VERO (African green monkey kidney) cell line Compound was tested for in vitro cytotoxicity against VERO (African green monkey kidney) cell line	10.1016/S0960-894X(97)00169-8
Vero	IC₅₀	46 nM Compound: Artemisinin	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in African green monkey (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in African green monkey (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs	[PMID: 19833520]
Vero	IC₅₀	4 ng/mL Compound: Artemisinin	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in African green monkey (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs by Malstat reagent method Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 infected in African green monkey (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs by Malstat reagent method	[PMID: 19914074]
Vero	IC₅₀	6 ng/mL Compound: Artemisinin	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in African green monkey (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs by Malstat reagent method Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 infected in African green monkey (Cercopithecus aethiops) Vero cells assessed as parasite LDH activity after 72 hrs by Malstat reagent method	[PMID: 19914074]
Vero	IC₅₀	> 10 μg/mL Compound: artemisinin	Cytotoxicity against african green monkey kidney Vero cells Cytotoxicity against african green monkey kidney Vero cells	[PMID: 15165136]
Vero	IC₅₀	> 16.86 μM Compound: Artemisinin	Cytotoxicity against African green monkey Vero cells after 48 hrs by neutral red uptake assay Cytotoxicity against African green monkey Vero cells after 48 hrs by neutral red uptake assay	[PMID: 27936446]
Vero	IC₅₀	> 4.76 μg/mL Compound: artemisinin	Cytotoxicity against african green monkey Vero cells after 48 hrs by neutral red assay Cytotoxicity against african green monkey Vero cells after 48 hrs by neutral red assay	[PMID: 19105653]
Vero	IC₅₀	> 4.7 μg/mL Compound: artemisinin	Cytotoxicity against Vero cells Cytotoxicity against Vero cells	[PMID: 17134906]
XF498	IC₅₀	16.2 μM Compound: 1a	Anticancer activity against human XF498 cells by sulforhodamine B assay Anticancer activity against human XF498 cells by sulforhodamine B assay	[PMID: 19819696]
leukaemia cell line	IC₅₀	>= 40 μM Compound: Artemisinin	Antiproliferative activity against human leukaemia cell line Antiproliferative activity against human leukaemia cell line	[PMID: 31945642]

In Vitro

Artemisinin (Qinghaosu) (25 or 50 μM; 24 hours) concentration-dependently suppresses Aβ25-35 induced cytotoxicity in PC12 cells^[1].
Artemisinin (1-100 μM; 24 hours) selectively inhibits cancer cell growth in a dose-dependent manner with IC₅₀ values of 31.30 ± 0.73 μM in UMRC-2 cells and 23.97 ± 0.92 CAKI-2 cells^[2].
Artemisinin (25, 50 μM; 24 hours) suppresses the phosphorylation of AKT in UMRC-2 and CAKI-2 cells in a dose-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	PC12 cells
Concentration:	25 or 50 μM
Incubation Time:	24 hours
Result:	Protected and rescue PC12 cells against Aβ25-35-induced cell death.

Cell Viability Assay^[2]

Cell Line:	RCC cells, RCC cell lines UMRC-2 and CAKI-2, and normal renal cell HK-2
Concentration:	1, 5, 10, 50, and 100 μM
Incubation Time:	24 hours
Result:	Selectively inhibited cancer cell growth in a dose-dependent manner.

Western Blot Analysis^[2]

Cell Line:	UMRC-2 and CAKI-2 cells
Concentration:	25, 50 μM
Incubation Time:	24 hours
Result:	Decreased pAKT in a dose-dependent manner.

In Vivo

Artemisinin (gavage; 20 mg/kg/day; for two weeks) suppresses UMRC-2 xenograft tumor growth^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	4-6 weeks old male nude mice^[2]
Dosage:	20 mg/kg
Administration:	gavage; every day for two weeks
Result:	Suppressed UMRC-2 xenograft tumor growth.

Clinical Trial

Molecular Weight

282.33

Formula

C₁₅H₂₂O₅

CAS No.

63968-64-9

Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@H](C)[C@]2([H])CC[C@@H](C)[C@]3([H])CC[C@@](O4)(C)OO[C@]32[C@]4([H])O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (177.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5420 mL	17.7098 mL	35.4195 mL
5 mM	0.7084 mL	3.5420 mL	7.0839 mL
10 mM	0.3542 mL	1.7710 mL	3.5420 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (287 KB) SDS (393 KB)

COA (260 KB) HNMR (261 KB) MS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Zeng Z, et al. Artemisinin protects PC12 cells against β-amyloid-induced apoptosis through activation of the ERK1/2 signaling pathway. Redox Biol. 2017 Apr 4;12:625-633. [Content Brief]

[2]. Lin SP, et al. Artemisinin Prevents Glutamate-Induced Neuronal Cell Death Via Akt Pathway Activation. Front Cell Neurosci. 2018 Apr 20;12:108. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5420 mL	17.7098 mL	35.4195 mL	88.5489 mL
	5 mM	0.7084 mL	3.5420 mL	7.0839 mL	17.7098 mL
	10 mM	0.3542 mL	1.7710 mL	3.5420 mL	8.8549 mL
	15 mM	0.2361 mL	1.1807 mL	2.3613 mL	5.9033 mL
	20 mM	0.1771 mL	0.8855 mL	1.7710 mL	4.4274 mL
	25 mM	0.1417 mL	0.7084 mL	1.4168 mL	3.5420 mL
	30 mM	0.1181 mL	0.5903 mL	1.1807 mL	2.9516 mL
	40 mM	0.0885 mL	0.4427 mL	0.8855 mL	2.2137 mL
	50 mM	0.0708 mL	0.3542 mL	0.7084 mL	1.7710 mL
	60 mM	0.0590 mL	0.2952 mL	0.5903 mL	1.4758 mL
	80 mM	0.0443 mL	0.2214 mL	0.4427 mL	1.1069 mL
	100 mM	0.0354 mL	0.1771 mL	0.3542 mL	0.8855 mL

Artemisinin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Artemisinin63968-64-9Qinghaosu NSC 369397NSC369397NSC 369397NSC-369397HCVParasiteAktFerroptosisHepatitis C virusPKBProtein kinase BInhibitorinhibitorinhibit

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Artemisinin (Synonyms: Qinghaosu; NSC 369397)

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