2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. ATP disodium salt hydrate

ATP (disodium salt hydrate)  (Synonyms: Adenosine 5'-triphosphate (disodium salt hydrate); アデノシン5′-三リン酸 二ナトリウム塩 水和物)

製品番号: HY-W010735 純度: 99.80%
COA 取扱説明書 Technical Support

ATP disodium salt hydrate (Adenosine 5'-triphosphatedisodium salt hydrate) is a central component of energy storage and metabolism in vivo, provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt hydrate is an important endogenous signaling molecule in immunity and inflammation.

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CAS 番号 : 34369-07-8

容量 価格（税別） 在庫状況 数量
500 mg $50 在庫あり
1 g $60 在庫あり
5 g $120 在庫あり
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カスタマーレビュー

Based on 146 publication(s) in Google Scholar

Other Forms of ATP disodium salt hydrate:

ATP (disodium salt hydrate) 関連抗体

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顧客検証

WB
Cell Imaging/Staining
RT-PCR
IP
Cell Proliferation/Viability Assay

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    BV2 cells (2.5 × 10⁵ cells/ml) were pretreated with syringin for 2 h and subsequently stimulated with 100 ng/ml LPS for 16 h. For activation, LPS‑primed cells were treated with syringin or MCC950 for 2 h, followed by 3 mM ATP for 1.5 h or 10 μM nigericin for 1 h before collection. Representative WB showing NLRP3, pro-Caspase-1, and pro-IL-1β in cell lysates, and cleaved-Caspase-1 and cleaved-IL-1β in culture supernatants in ATP-induced activation. (B, C) Densitometric quantification of cleaved-Caspase-1 and cleaved-IL-1β in (A).

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    LPS (3 mM) + ATP treatment significantly increased Iba-1 intensity, indicating activation of BV2 cells.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    qRT-PCR analysis of a panel of microglial activation markers revealed that LPS + ATP (3 mM) significantly upregulated the transcription of CD11b, CD14, CD16, CD32, and Iba-1 in BV2 cell.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    DCFH-DA fluorescence staining revealed that LPS + ATP (3 mM) treatment markedly increased intracellular ROS levels in BV2 microglia, as indicated by elevated DCF fluorescence intensity. Treatment with 50 or 100 μM syringin significantly suppressed ROS production.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    100 μM syringin did not affect the expression levels of NLRP3, pro-Caspase-1, or ASC proteins in total lysates under LPS + ATP treatment in BV2 cells.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11.  [Abstract]

    Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Transl Res. 2023 May:255:1-13.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2022 Feb;29(2):439-450.  [Abstract]

    Primary BMDM cells were isolated from WT and Trim21-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356.  [Abstract]

    Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.

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    製品説明

    ATP disodium salt hydrate (Adenosine 5'-triphosphatedisodium salt hydrate) is a central component of energy storage and metabolism in vivo, provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt hydrate is an important endogenous signaling molecule in immunity and inflammation[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    体外実験

    ATP disodium salt hydrate (5mM; 1 hour) co-treatment with LPS (1 μg/ml) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs[3].
    ATP disodium salt hydrate (2 mM; 0.5-24 hours) induces secretion of interleukin 1β, KC and MIP-2 from bone marrow derived macrophages (BMDMs) in vitro in a caspase-1 activation-dependent manner[4].
    ATP disodium salt hydrate stimulats cytokine and chemokine secretion and inflammasome activation directly and indirectly induces in vitro neutrophil chemotaxis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP (disodium salt hydrate) 関連抗体
    体内実験

    ATP disodium salt hydrate (50 mg/kg; i.p.) protects mice against bacterial infection in vivo[4].
    ATP disodium salt hydrate induces the secretion of IL1β, KC and MIP-2 and neutrophils recruitment in vivo[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, before bacterial (E. coli) challenge
    Result: Protected mice from bacterial infection.
    分子式

    C10H14N5O13Na2P3.xH2O
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OP(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O1)(OP(OP(O)(O[Na])=O)(O[Na])=O)=O.[H]O[H].[x]
    Structure Classification
    Initial Source

    widespread
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : 100 mg/mL (Need ultrasonic)

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    一般には略語で表示されます：C1V1 = C2V2

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    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL; Clear solution; Need ultrasonic

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    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
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    純度とドキュメンテーション

    純度: 99.80%

    COA (252 KB) HNMR (286 KB) RP-HPLC (309 KB)

    取扱説明書 (2659 KB)
    参考文献
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    ATP disodium salt hydrate Related Classifications

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP disodium salt34369-07-8Adenosine 5'-triphosphate disodium saltEndogenous MetaboliteenergystoragemetabolismimmunityinflammationNLRP3inflammasomecytokineInhibitorinhibitorinhibit

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    製品名:
    ATP disodium salt hydrate
    製品番号:
    HY-W010735
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