2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. ATP disodium salt hydrate

ATP disodium salt hydrate  (Synonyms: Adenosine 5'-triphosphate disodium salt hydrate)

Cat. No.: HY-W010735 Pureza: 99.80%
COA
SDS
Instrucciones de manejo Technical Support

ATP disodium salt hydrate (Adenosine 5'-triphosphatedisodium salt hydrate) is a central component of energy storage and metabolism in vivo, provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt hydrate is an important endogenous signaling molecule in immunity and inflammation.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 34369-07-8

Tamaño Precio Stock Cantidad
500 mg En stock
1 g En stock
5 g En stock
10 g   Obtener un presupuesto  
50 g   Obtener un presupuesto  

* Seleccione Cantidad antes de agregar artículos.

This product is a controlled substance and not for sale in your territory.

Revisión del cliente

Based on 146 publication(s) in Google Scholar

Other Forms of ATP disodium salt hydrate:

ATP disodium salt hydrate Related Antibodies

Top Publications Citing Use of Products

146 Publications Citing Use of MCE ATP disodium salt hydrate

WB
Cell Imaging/Staining
RT-PCR
IP
Cell Proliferation/Viability Assay

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    BV2 cells (2.5 × 10⁵ cells/ml) were pretreated with syringin for 2 h and subsequently stimulated with 100 ng/ml LPS for 16 h. For activation, LPS‑primed cells were treated with syringin or MCC950 for 2 h, followed by 3 mM ATP for 1.5 h or 10 μM nigericin for 1 h before collection. Representative WB showing NLRP3, pro-Caspase-1, and pro-IL-1β in cell lysates, and cleaved-Caspase-1 and cleaved-IL-1β in culture supernatants in ATP-induced activation. (B, C) Densitometric quantification of cleaved-Caspase-1 and cleaved-IL-1β in (A).

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    LPS (3 mM) + ATP treatment significantly increased Iba-1 intensity, indicating activation of BV2 cells.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    qRT-PCR analysis of a panel of microglial activation markers revealed that LPS + ATP (3 mM) significantly upregulated the transcription of CD11b, CD14, CD16, CD32, and Iba-1 in BV2 cell.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    DCFH-DA fluorescence staining revealed that LPS + ATP (3 mM) treatment markedly increased intracellular ROS levels in BV2 microglia, as indicated by elevated DCF fluorescence intensity. Treatment with 50 or 100 μM syringin significantly suppressed ROS production.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 25:148:157454.  [Abstract]

    100 μM syringin did not affect the expression levels of NLRP3, pro-Caspase-1, or ASC proteins in total lysates under LPS + ATP treatment in BV2 cells.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11.  [Abstract]

    Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Transl Res. 2023 May:255:1-13.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2022 Feb;29(2):439-450.  [Abstract]

    Primary BMDM cells were isolated from WT and Trim21-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356.  [Abstract]

    Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

    ATP disodium salt hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.

    Ver todos los productos específicos de isoformas Endogenous Metabolite:

    Ver todas las isoformas
    Human Endogenous Metabolite Microbial Metabolite

    • Actividad biológica

    • Pureza y Documentación

    • Referencias

    • Revisión del cliente

    Descripciòn

    ATP disodium salt hydrate (Adenosine 5'-triphosphatedisodium salt hydrate) is a central component of energy storage and metabolism in vivo, provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt hydrate is an important endogenous signaling molecule in immunity and inflammation[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    ATP disodium salt hydrate (5mM; 1 hour) co-treatment with LPS (1 μg/ml) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs[3].
    ATP disodium salt hydrate (2 mM; 0.5-24 hours) induces secretion of interleukin 1β, KC and MIP-2 from bone marrow derived macrophages (BMDMs) in vitro in a caspase-1 activation-dependent manner[4].
    ATP disodium salt hydrate stimulats cytokine and chemokine secretion and inflammasome activation directly and indirectly induces in vitro neutrophil chemotaxis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP disodium salt hydrate Related Antibodies
    In Vivo

    ATP disodium salt hydrate (50 mg/kg; i.p.) protects mice against bacterial infection in vivo[4].
    ATP disodium salt hydrate induces the secretion of IL1β, KC and MIP-2 and neutrophils recruitment in vivo[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, before bacterial (E. coli) challenge
    Result: Protected mice from bacterial infection.
    Fòrmula

    C10H14N5O13Na2P3.xH2O
    No. CAS
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OP(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O1)(OP(OP(O)(O[Na])=O)(O[Na])=O)=O.[H]O[H].[x]
    Structure Classification
    Initial Source

    widespread
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvente y solubilidad
    In Vitro: 

    H2O : 100 mg/mL (Need ultrasonic)

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL; Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureza y Documentación

    Purity: 99.80%

    SDS (393 KB)

    COA (252 KB) HNMR (286 KB) RP-HPLC (309 KB)

    Instrucciones de manejo (2659 KB)
    Referencias
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    ATP disodium salt hydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP disodium salt34369-07-8Adenosine 5'-triphosphate disodium saltEndogenous MetaboliteenergystoragemetabolismimmunityinflammationNLRP3inflammasomecytokineInhibitorinhibitorinhibit

    Productos vistos recientemente:

    Consulta en línea

    Your information is safe with us. * Required Fields.

    Nombre del producto

    		  

    Requested Quantity *

    Nombre del solicitante *

    		 

    Saludo

    Direcciòn del E-mail *

    		 

    Número de teléfono *

    Department

    		 

    Nombre de la Organizaciòn *

    City

    Provincia

    Country or Region *

    		      

    Observaciones

    Consulta para venta a granel

    Inquiry Information

    Nombre del producto:
    ATP disodium salt hydrate
    Cat. No.:
    HY-W010735
    Cantidad:
    MCE Japan Authorized Agent:

    Revisión del cliente

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Nombre del producto

    		  

    Lot #

    Nombre del solicitante *

    		 

    Direcciòn del E-mail *

    Número de teléfono

    		 

    Department *

    Nombre de la Organizaciòn *

    		 

    Country or Region *

    Observaciones

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.