2. Others Apoptosis Metabolic Enzyme/Protease
  3. Ferroptosis Environmental Pollutants Endogenous Metabolite
  4. Butylated hydroxytoluene

Butylated hydroxytoluene  (Synonyms: 2,6-ジ-tert-ブチル-4-メチルフェノール)

製品番号: HY-Y0172 純度: 99.74%
COA 取扱説明書 Technical Support

Butylated hydroxytoluene is an antioxidant widely used in foods and in food-related products. Butylated hydroxytoluene is a Ferroptosis inhibitor.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 128-37-0

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
100 mg $55 在庫あり
500 mg $88 在庫あり
1 g $105 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Other Forms of Butylated hydroxytoluene:

Butylated hydroxytoluene 関連抗体

Top Publications Citing Use of Products

    Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529.  [Abstract]

    Viability of HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

    Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529.  [Abstract]

    MDA levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

    Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529.  [Abstract]

    4-HNE levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

    Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529.  [Abstract]

    ROS levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

    Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529.  [Abstract]

    Representative images of C11-BODIPY probe in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Butylated hydroxytoluene is an antioxidant widely used in foods and in food-related products[1]. Butylated hydroxytoluene is a Ferroptosis inhibitor[2].

    Cellular Effect
    Cell Line Type Value Description References
    Hepatocyte IC50
    1.7 μM
    Compound: 26
    Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes
    Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes
    		[PMID: 15546710]
    HL-60 IC50
    3.18 μM
    Compound: table 1, 31(BHT)
    Induction of DNA fragmentation in human HL60 cells after 16 hrs
    Induction of DNA fragmentation in human HL60 cells after 16 hrs
    		[PMID: 16279782]
    L1210 IC50
    91.2 μM
    Compound: table 1, 31(BHT)
    Cytotoxicity against mouse L1210 cells
    Cytotoxicity against mouse L1210 cells
    		[PMID: 16279782]
    Platelet IC50
    14.5 μM
    Compound: butylated hydroxy toulene
    Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control
    Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control
    		[PMID: 9358644]
    体内実験

    Butylhydroxytoluene (BHT) is well-known as a potent promoter of carcinogen-induced lung tumors in mice. Butylated hydroxytoluene (orally; 400 mg/kg; weekly) administration increases the susceptibility of 7-week-old rasH2 mice to lung carcinogens[3]

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    220.36

    分子式

    C15H24O
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    OC1=C(C(C)(C)C)C=C(C)C=C1C(C)(C)C
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    RT, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (RT, stored under nitrogen)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (453.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5380 mL 22.6901 mL 45.3803 mL
    5 mM 0.9076 mL 4.5380 mL 9.0761 mL
    10 mM 0.4538 mL 2.2690 mL 4.5380 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (11.35 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (453.80 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.93%

    COA (249 KB) HNMR (109 KB) GC (195 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.5380 mL 22.6901 mL 45.3803 mL 113.4507 mL
    5 mM 0.9076 mL 4.5380 mL 9.0761 mL 22.6901 mL
    10 mM 0.4538 mL 2.2690 mL 4.5380 mL 11.3451 mL
    15 mM 0.3025 mL 1.5127 mL 3.0254 mL 7.5634 mL
    20 mM 0.2269 mL 1.1345 mL 2.2690 mL 5.6725 mL
    25 mM 0.1815 mL 0.9076 mL 1.8152 mL 4.5380 mL
    30 mM 0.1513 mL 0.7563 mL 1.5127 mL 3.7817 mL
    40 mM 0.1135 mL 0.5673 mL 1.1345 mL 2.8363 mL
    50 mM 0.0908 mL 0.4538 mL 0.9076 mL 2.2690 mL
    60 mM 0.0756 mL 0.3782 mL 0.7563 mL 1.8908 mL
    80 mM 0.0567 mL 0.2836 mL 0.5673 mL 1.4181 mL
    100 mM 0.0454 mL 0.2269 mL 0.4538 mL 1.1345 mL
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    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Butylated hydroxytoluene Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Butylated hydroxytoluene128-37-0FerroptosisEnvironmental PollutantsEndogenous MetaboliteEnvironmental ContaminantsInhibitorinhibitorinhibit

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    製品名:
    Butylated hydroxytoluene
    製品番号:
    HY-Y0172
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