Diallyl Trisulfide

Name: Diallyl Trisulfide
Brand: MedChemExpress
SKU: HY-117235
Rating: 5.0 (3 reviews)

製品番号: HY-117235 純度: 99.9%: Data Sheet SDS COA 取扱説明書
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Diallyl Trisulfide is an orally active anticancer agent that can be isolated from garlic. Diallyl Trisulfide has the ability to induce apoptosis and exhibits anticancer, anti-inflammatory, antioxidant, and antibacterial activities. Diallyl Trisulfide can be used to study a variety of cancers, including liver, colon and prostate cancer.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Diallyl Trisulfide 構造式

CAS 番号 : 2050-87-5

容量	価格（税別）	在庫状況	数量
25 mg	$185	在庫あり	Estimated Time of Arrival: December 31
50 mg	$312	在庫あり	Estimated Time of Arrival: December 31
100 mg	$503	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 3 publication(s) in Google Scholar

Other Forms of Diallyl Trisulfide:

Diallyl Trisulfide (Standard) お問い合わせ

顧客検証

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

Interleukin Related アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

IL-6

IL-8

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.49 μM Compound: 4	Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels	[PMID: 20356305]
MDCK	IC₅₀	0.05 mM Compound: 3	Cytotoxicity against MDCK cells assessed as decrease in cell viability after 16 hrs by tetrazolium salt WST-1 assay Cytotoxicity against MDCK cells assessed as decrease in cell viability after 16 hrs by tetrazolium salt WST-1 assay	[PMID: 23163425]

体外実験

Diallyl Trisulfide (25-100 μM; 24-72 h) induces apoptosis and inhibits cell proliferation in A549 cells, exhibiting anticancer activity^[1].
Diallyl Trisulfide (5-10 μM; 1 h) significantly inhibits naphthalene (20 μM)-stimulated ROS generation and reduces the levels of inflammatory factors IL-6, TNF-α, and IL-8 by increasing superoxide dismutase (SOD) activity, thereby possessing antioxidant and anti-inflammatory activities^[2].
Diallyl Trisulfide (93.75-375 µM; 24 h) attenuates H9N2 avian influenza virus (AIV) infection in human lung A549 epithelial cells, demonstrating antiviral activity^[3].
Diallyl Trisulfide inhibits the growth of Penicillium expansum with antifungal activity (minimum fungicidal concentration (MFC)₉₉ value: ≤ 90 μg/mL)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[3]

Cell Line:	A549
Concentration:	75 μM, 93.75 μM, 125 μM, 125 μM, 187.5 μM, 250 μM, 375 μM, 500 μM
Incubation Time:	48 h
Result:	Was not cytotoxic to A549 cells.

Cell Viability Assay^[1]

Cell Line:	A549
Concentration:	25 μM, 50 μM, 100 μM
Incubation Time:	24 h
Result:	Significantly inhibited cell activity in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	A549
Concentration:	25 μM, 50 μM, 100 μM
Incubation Time:	24 h; 48 h; 72 h
Result:	Significantly reduced the expression of Bcl-2 protein at a dose of 100 μM for 48 h. significantly increased the expression of Bax protein.

RT-PCR^[1]

Cell Line:	A549
Concentration:	100 μM
Incubation Time:	48 h
Result:	Increased the mRNA expression of caspase-3, -8, and -9. Significantly increased the expression of Bax mRNA.

Real Time qPCR^[3]

Cell Line:	A549
Concentration:	375 µM
Incubation Time:	24 h, 48 h
Result:	Increased the expression of antiviral factors RIG-I, IRF-3 and IFN-β.

体内実験

Diallyl Trisulfide (6 μM/animal; Oral gavage, every other day for 30 days) inhibits tumor growth and exhibits anticancer activity in BALB/c nude mice^[1].
Diallyl Trisulfide (20-80 mg/kg; Oral gavage, single dose) demonstrates antioxidant and anti-inflammatory activities in Kunming mice induced by naphthalene (100 mg/kg; orally, single dose)^[2].
Diallyl Trisulfide (30 mg/kg; intraperitoneal injection; once daily for 2 weeks) reduces lung edema and inflammation caused by H9N2 AIV infection in BABL/c mice, exhibiting antiviral activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Kunming mouse model of inflammation induced by naphthalene^[2]
Dosage:	20 mg/kg, 40 mg/kg, 80 mg/kg
Administration:	Oral gavage (p.o.); Single dose. Before naphthalene treatment (100 mg/kg; p.o., single dose)
Result:	Inhibited the production of serum nitric oxide (NO) and pulmonary myeloperoxidase (MPO). Significantly reduced the area of inflammatory cell infiltration induced by naphthalene and alleviated lung injury in mice.

Animal Model:	BABL/c mice model infected with H9N2 AIV^[3]
Dosage:	30 mg/kg
Administration:	Intraperitoneal injection (i.p.); Once daily for two weeks
Result:	Reduced the severity of pulmonary edema. Significantly reduced viral load in mouse lungs.

分子量

178.34

分子式

C₆H₁₀S₃

CAS 番号

2050-87-5

Appearance

Liquid (Density: 1.085 g/cm³)

Color

Colorless to light yellow

SMILES

C=CCSSSCC=C

Structure Classification

Others

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (560.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6073 mL	28.0363 mL	56.0727 mL
5 mM	1.1215 mL	5.6073 mL	11.2145 mL
10 mM	0.5607 mL	2.8036 mL	5.6073 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (14.02 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.9%

Select Batch:

データシート (279 KB) SDS (421 KB)

COA (241 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Li W, et al. Diallyl trisulfide induces apoptosis and inhibits proliferation of A549 cells in vitro and in vivo. Acta Biochim Biophys Sin (Shanghai). 2012 Jul;44(7):577-83. [Content Brief]

[2]. Zhang F, et al. Diallyl trisulfide inhibits naphthalene-induced oxidative injury and the production of inflammatory responses in A549 cells and mice. Int Immunopharmacol. 2015 Dec;29(2):326-333. [Content Brief]

[3]. Ming L, et al. Antiviral activity of diallyl trisulfide against H9N2 avian influenza virus infection in vitro and in vivo. Virol J. 2021 Aug 19;18(1):171. [Content Brief]

[4]. Pu Liu, et al. Diallyl trisulfide (DATS) effectively induced apoptosis of postharvest diseasePenicillium expansum of citrus. Annals of Microbiology. December 2009, Volume 59, Issue 4, pp 675-679.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6073 mL	28.0363 mL	56.0727 mL	140.1817 mL
	5 mM	1.1215 mL	5.6073 mL	11.2145 mL	28.0363 mL
	10 mM	0.5607 mL	2.8036 mL	5.6073 mL	14.0182 mL
	15 mM	0.3738 mL	1.8691 mL	3.7382 mL	9.3454 mL
	20 mM	0.2804 mL	1.4018 mL	2.8036 mL	7.0091 mL
	25 mM	0.2243 mL	1.1215 mL	2.2429 mL	5.6073 mL
	30 mM	0.1869 mL	0.9345 mL	1.8691 mL	4.6727 mL
	40 mM	0.1402 mL	0.7009 mL	1.4018 mL	3.5045 mL
	50 mM	0.1121 mL	0.5607 mL	1.1215 mL	2.8036 mL
	60 mM	0.0935 mL	0.4673 mL	0.9345 mL	2.3364 mL
	80 mM	0.0701 mL	0.3505 mL	0.7009 mL	1.7523 mL
	100 mM	0.0561 mL	0.2804 mL	0.5607 mL	1.4018 mL