Ca2+ Indicator Probes

Ca2+ Indicator Probes (44):

Cat. No.	Product Name	CAS No.	Purity	Chemical Structure

Fluo-4 AM	273221-67-3	≥99.0%
Fluo-4 AM is a cell-permeable Ca²⁺ (calcium ion) indicator (Ex/Em = 485/526 nm).

Fura-2 AM	108964-32-5	≥99.0%
Fura-2 AM is a membrane permeable, intracellular, UV light-excitable and ratiometric fluorescent Ca²⁺ (calcium ion) indicator. Fura-2 AM crosses cell membranes and is converted to Fura-2 (HY-D0110A) via cellular esterases. Fura-2 AM can be used to detect calcium in cells.

Fluo-3AM	121714-22-5	≥99.0%
Fluo-3 AM is a fluorecent Ca²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

Calcein (mixture of isomers)	154071-48-4
Calcein (mixture of isomers), Calcein (HY-D0040) Derivative, is a fluorescent dye (Ex/Em = 495/515 nm). Calcein (mixture of isomers) can be used for the researches for live cell staining, calcium ion detection, bone fluorescence labeling and membrane permeability leakage detection.

Indo-1 AM	112926-02-0
Calcium is an important part of the human body, usually in the form of calcium, a large number of bones and teeth of the human body, a small amount of blood and tissues. MCE calcium ion detection probe can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence, in addition, Mag-indo-1/AM and Mag-Fluo-4 AM at a certain concentration (usually 5 mM) can effectively identify intracellular magnesium ions.

Fluo-3AM (solution)	121714-22-5
Fluo-3AM (solution) is a fluorecent Ca²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence. Solvent and concentration: DMSO: 5 mM

TCM-4COOH

BOCA-1-BG	1236310-96-5
BOCA-1-BG is a BODIPY-labeled SNAP tag fluorescence probe. BOCA-1-BG can be used to detect Ca²⁺ concentration and serves as a highly sensitive Ca²⁺ fluorescent indicator.

Calcium Green 1AM	186501-28-0
Calcium Green 1AM is a cell-permeant fluorescent calcium indicator (Excitation 506 nm; Emission 531 nm). Calcium Green 1AM is converted to the fluorescent calcium indicator by intracellular esterases.

OG 488, SE	198139-51-4
OG 488, SE (Oregon green 488 succinimidyl ester), a fluorescent pH indicator, has many applications in biochemistry and neurosciences.

Calcium Orange AM	172646-19-4	98%
Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles.

Fura-2 pentapotassium	113694-64-7
Fura-2 pentapotassium is a cell-impermeable scaled fluorescent dye that can be used for intracellular calcium imaging. Fura-2 pentapotassium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca²⁺ level.

Calmagite	3147-14-6	98.0%
Calmagite is a chromomeric reagent which can be used to detect calcium and magnesium in various samples.

INDO 1 pentapotassium	132319-56-3
INDO 1 pentapotassium is a cell-impermeant fluorescent Ca²⁺ indicator, binds specifically to Ca²⁺ while emitting fluorescence, the maximum emission wavelength shifts from 485 nm before binding to 410 nm.

Fura-FF pentapotassium	192140-58-2	99%
Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration).

Calcium Green-5N AM	216699-33-1
Calcium Green-5N AM is a Ca²⁺-sensitive fluorescent probe (K_d = 14 μM). Calcium Green-5N AM shows enhanced fluorescence intensity upon binding to Ca²⁺, and it can monitor free Ca²⁺ in neurons.

Quin-2AM	83104-85-2
Quin-2AM is a fluorecent Ca²⁺ chelator, with high affinity for calcium. Quin-2AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

BAPTA-TMFM	96315-11-6
BAPTA-TMFM is a fluorescent chelating indicator used to study the role of cytosolic free calcium.

Calcium green-5N hexapotassium	153130-66-6
Calcium green-5N hexapotassium is a cell-impermeant Ca²⁺-sensitive fluorescent probe. Calcium green-5N hexapotassium shows enhancement in fluorescence intensity on binding Ca²⁺, and can monitor free Ca²⁺ in neurons.

INDO 1 pentasodium
INDO 1 pentasodium is a cell-impermeant ratiometric, free calcium ion (Ca²⁺) fluorescent indicator that can quantitatively monitor dynamic changes in intracellular free Ca²⁺ concentrations. When unbound to Ca²⁺ (free state), INDO 1 pentasodium exhibits a peak emission wavelength of 485 nm under UV excitation (350 nm). Upon binding to Ca²⁺ (bound state), the emission peak shifts to 405 nm. INDO 1 pentasodium is highly photolabile and susceptible to photobleaching, and its emission spectrum may overlap with the autofluorescence of NADH.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

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