Fura-FF pentapotassium
Based on 1 Customer Validation
Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration).
For research use only. We do not sell to patients.
- Purity: 99%
- CAS No.: 192140-58-2
- Formula: C28H18F2K5N3O14
- Molecular Weight:853.94
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Human Sperm Cells:
[2] 1. Incubate the cells according to your normal protocol.
2. Load cells with 5 μM Fura-FF in 1 mL Hepes-buffered human sperm (H-HSM) medium at 36 °C for 40 min.
3. Wash the sample by centrifugation and scatter cells in disposable centrifuge tubes at 107 cells in 3 mL of H-HSM and maintained at 36 °C.
4. Immediately measure fluorescence recordings.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 192140-58-2
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Appearance Solid
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Molecular Weight 853.94
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Formula C28H18F2K5N3O14
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Color White to light yellow
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SMILES
O=C(C1=CN=C(C2=CC3=CC(OCCOC4=C(N(CC(O[K])=O)CC(O[K])=O)C=CC(F)=C4F)=C(N(CC(O[K])=O)CC(O[K])=O)C=C3O2)O1)O[K]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ruggiu AA, et al. Fura-2FF-based calcium indicator for protein labeling. Org Biomol Chem. 2010 Aug 7;8(15):3398-401. [Content Brief]
[2]. Neri-Vidaurri Pdel C, et al. A remarkable increase in the pHi sensitivity of voltage-dependent calcium channels occurs in human sperm incubated in capacitating conditions. Biochem Biophys Res Commun. 2006 Apr 28;343(1):105-9. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fura-FF pentapotassium Related Classifications
HY-135239 Related Classifications
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