Fura-FF pentapotassium 

Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration)^[1].

Guidelines^[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Human Sperm Cells:
1. Incubate the cells according to your normal protocol.
2. Load cells with 5 μM Fura-FF in 1 mL Hepes-buffered human sperm (H-HSM) medium at 36 ℃ for 40 min.
3. Wash the sample by centrifugation and scatter cells in disposable centrifuge tubes at 1 10⁷ cells in 3 mL of H-HSM and maintained at 36 ℃.
4. Immediately measure fluorescence recordings.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

853.94

C₂₈H₁₈F₂K₅N₃O₁₄

192140-58-2

514

365

O=C(C1=CN=C(C2=CC3=CC(OCCOC4=C(N(CC(O[K])=O)CC(O[K])=O)C=CC(F)=C4F)=C(N(CC(O[K])=O)CC(O[K])=O)C=C3O2)O1)O[K]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ruggiu AA, et al. Fura-2FF-based calcium indicator for protein labeling. Org Biomol Chem. 2010 Aug 7;8(15):3398-401.  [Content Brief]

  [2]. Neri-Vidaurri Pdel C, et al. A remarkable increase in the pHi sensitivity of voltage-dependent calcium channels occurs in human sperm incubated in capacitating conditions. Biochem Biophys Res Commun. 2006 Apr 28;343(1):105-9.  [Content Brief]