Fura-2FF-based calcium indicator for protein labeling

  • Org Biomol Chem. 2010 Aug 7;8(15):3398-401. doi: 10.1039/c000158a.
Agostina A Ruggiu  1 ,  Michael Bannwarth ,  Kai Johnsson
Affiliations
  • 1. Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
PMID: 20556282 DOI: 10.1039/c000158a
Abstract

We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent CA(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its CA(2+) sensing ability after coupling to protein.

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