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Calmagite is a chromomeric reagent which can be used to detect calcium and magnesium in various samples.

For research use only. We do not sell to patients.

Calmagite

Calmagite Chemical Structure

CAS No. : 3147-14-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
100 mg In-stock
500 mg In-stock
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Based on 1 publication(s) in Google Scholar

Calmagite Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Calmagite is a chromomeric reagent which can be used to detect calcium and magnesium in various samples[1][2][3].

Cellular Effect
Cell Line Type Value Description References
BaF3 EC50
3 μM
Compound: 5
Effective concentration for thrombopoietin luciferase activity was determined in BAF-3 cells
Effective concentration for thrombopoietin luciferase activity was determined in BAF-3 cells
[PMID: 11606138]
In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of Calmagite Staining Solution[3]
40 mg Calmagite dissolved in 100 ml water
2. Analysis of biological materials
Serum: 50 μL serum was added to each tube directly, mixed with 2 mL buffer and 0.5 mL EGTA solution, and made up to 4 mL with water. Finally 1 mL Calmagite solution was added for color development, In addition, two standards containing 1 and 1.5 μg Mg2+ were always run parallel to check the dependability of the earlier standard curve.
3. Evaluate and compare the magnesium levels in serum and saliva of individuals with tobacco habits with and without potentially malignant disorders[2]
3.1 Serum collection
Two millilitres of blood was collected in plain red vacutainers by venipuncture using aseptic measures. At 3500 rpm, the serum was separated by centrifugation for 10 min.
3.2 Unstimulated saliva collection
Three millilitres of unstimulated whole saliva sample was collected from the mouth directly into the Eppendorf on a single occasion to press their tongue against their palate so that freshly secreted saliva was collected in the sublingual region for 5 min. Saliva was centrifuged at 3500 rpm for 10 min to remove the excess mucus and unwanted particles.
3.3 Serum and saliva estimation
The estimation of magnesium in serum and saliva was done by calmagite method.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Calmagite Related Antibodies
Molecular Weight

358.37

Formula

C17H14N2O5S
CAS No.
Appearance

Solid

Color

Light brown to black

SMILES

O=S(C1=C2C=CC=CC2=C(/N=N/C3=CC(C)=CC=C3O)C(O)=C1)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (93.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 7.14 mg/mL (19.92 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7904 mL 13.9521 mL 27.9041 mL
5 mM 0.5581 mL 2.7904 mL 5.5808 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.7904 mL 13.9521 mL 27.9041 mL 69.7603 mL
5 mM 0.5581 mL 2.7904 mL 5.5808 mL 13.9521 mL
10 mM 0.2790 mL 1.3952 mL 2.7904 mL 6.9760 mL
15 mM 0.1860 mL 0.9301 mL 1.8603 mL 4.6507 mL
DMSO 20 mM 0.1395 mL 0.6976 mL 1.3952 mL 3.4880 mL
25 mM 0.1116 mL 0.5581 mL 1.1162 mL 2.7904 mL
30 mM 0.0930 mL 0.4651 mL 0.9301 mL 2.3253 mL
40 mM 0.0698 mL 0.3488 mL 0.6976 mL 1.7440 mL
50 mM 0.0558 mL 0.2790 mL 0.5581 mL 1.3952 mL
60 mM 0.0465 mL 0.2325 mL 0.4651 mL 1.1627 mL
80 mM 0.0349 mL 0.1744 mL 0.3488 mL 0.8720 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Calmagite Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Calmagite3147-14-6Fluorescent DyeInhibitorinhibitorinhibit

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