Calmagite
Based on 1 Customer Validation
Calmagite is a chromomeric reagent which can be used to detect calcium and magnesium in various samples.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 3147-14-6
- Formula: C17H14N2O5S
- Molecular Weight:358.37
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| BaF3 | EC50 |
3 μM
Compound: 5
|
Effective concentration for thrombopoietin luciferase activity was determined in BAF-3 cells
Effective concentration for thrombopoietin luciferase activity was determined in BAF-3 cells
|
[PMID: 11606138] |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of Calmagite Staining Solution[3]
40 mg Calmagite dissolved in 100 ml water
2. Analysis of biological materials
Serum: 50 μL serum was added to each tube directly, mixed with 2 mL buffer and 0.5 mL EGTA solution, and made up to 4 mL with water. Finally 1 mL Calmagite solution was added for color development, In addition, two standards containing 1 and 1.5 μg Mg2+ were always run parallel to check the dependability of the earlier standard curve.
3. Evaluate and compare the magnesium levels in serum and saliva of individuals with tobacco habits with and without potentially malignant disorders[2]
3.1 Serum collection
Two millilitres of blood was collected in plain red vacutainers by venipuncture using aseptic measures. At 3500 rpm, the serum was separated by centrifugation for 10 min.
3.2 Unstimulated saliva collection
Three millilitres of unstimulated whole saliva sample was collected from the mouth directly into the Eppendorf on a single occasion to press their tongue against their palate so that freshly secreted saliva was collected in the sublingual region for 5 min. Saliva was centrifuged at 3500 rpm for 10 min to remove the excess mucus and unwanted particles.
3.3 Serum and saliva estimation
The estimation of magnesium in serum and saliva was done by calmagite method.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 3147-14-6
-
Appearance Solid
-
Molecular Weight 358.37
-
Formula C17H14N2O5S
-
Color Light brown to black
-
SMILES
O=S(C1=C2C=CC=CC2=C(/N=N/C3=CC(C)=CC=C3O)C(O)=C1)(O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 33.33 mg/mL (93.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 7.14 mg/mL (19.92 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Rasouli Z, et al. Simultaneously detection of calcium and magnesium in various samples by calmagite and chemometrics data processing. Spectrochim Acta A Mol Biomol Spectrosc. 2016 Dec 5;169:72-81. [Content Brief]
[2]. Singhal I, et al. Evaluation of magnesium levels in serum and saliva by calmagite method in individuals with tobacco habits with or without potentially malignant disorders. J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):425. [Content Brief]
[3]. Chauhan UP, et al. Use of calmagite for the determination of traces of magnesium in biological materials. Anal Biochem. 1969 Oct 15;32(1):70-80. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.7904 mL | 13.9521 mL | 27.9041 mL | 69.7603 mL |
| 5 mM | 0.5581 mL | 2.7904 mL | 5.5808 mL | 13.9521 mL | |
| 10 mM | 0.2790 mL | 1.3952 mL | 2.7904 mL | 6.9760 mL | |
| 15 mM | 0.1860 mL | 0.9301 mL | 1.8603 mL | 4.6507 mL | |
| DMSO | 20 mM | 0.1395 mL | 0.6976 mL | 1.3952 mL | 3.4880 mL |
| 25 mM | 0.1116 mL | 0.5581 mL | 1.1162 mL | 2.7904 mL | |
| 30 mM | 0.0930 mL | 0.4651 mL | 0.9301 mL | 2.3253 mL | |
| 40 mM | 0.0698 mL | 0.3488 mL | 0.6976 mL | 1.7440 mL | |
| 50 mM | 0.0558 mL | 0.2790 mL | 0.5581 mL | 1.3952 mL | |
| 60 mM | 0.0465 mL | 0.2325 mL | 0.4651 mL | 1.1627 mL | |
| 80 mM | 0.0349 mL | 0.1744 mL | 0.3488 mL | 0.8720 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Powered by Bioz