1. Dye Reagents

Indo-1 AM (Synonyms: Indo-1 Acetoxymethyl ester)

Cat. No.: HY-101898
Handling Instructions

Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

For research use only. We do not sell to patients.

Indo-1 AM Chemical Structure

Indo-1 AM Chemical Structure

CAS No. : 112926-02-0

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

In Vitro

Indo-1 AM is a fluorescent Ca2+ indicator with λex=340 nm and λem=405/485 nm[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus[2].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9902 mL 4.9509 mL 9.9019 mL
5 mM 0.1980 mL 0.9902 mL 1.9804 mL
10 mM 0.0990 mL 0.4951 mL 0.9902 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1009.91

Formula

C₄₇H₅₁N₃O₂₂

CAS No.

112926-02-0

SMILES

O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)N2)OCOC(C)=O

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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This equation is commonly abbreviated as: C1V1 = C2V2

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Product Name:
Indo-1 AM
Cat. No.:
HY-101898
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Indo-1 AM

Cat. No.: HY-101898