1. Dye Reagents
  2. Indo-1 AM

Indo-1 AM (Synonyms: Indo-1 Acetoxymethyl ester)

Cat. No.: HY-101898
Handling Instructions

Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

For research use only. We do not sell to patients.

Indo-1 AM Chemical Structure

Indo-1 AM Chemical Structure

CAS No. : 112926-02-0

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Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

In Vitro

Indo-1 AM is a fluorescent Ca2+ indicator with λex=340 nm and λem=405/485 nm[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus[2].

Molecular Weight









Room temperature in continental US; may vary elsewhere.


Please store the product under the recommended conditions in the Certificate of Analysis.

Cell Assay

The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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Indo-1 AMIndo-1 Acetoxymethyl esterOthersInhibitorinhibitorinhibit

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