Calcium Green-5N AM
Calcium Green-5N AM is a Ca2+-sensitive fluorescent probe (Kd = 14 μM). Calcium Green-5N AM shows enhanced fluorescence intensity upon binding to Ca2+, and it can monitor free Ca2+ in neurons.
For research use only. We do not sell to patients.
- CAS No.: 216699-33-1
- Formula: C59H52Cl2N4O282-
- Molecular Weight:1335.96
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1.\tIsolate Mitochondria: Prepare mitochondria from tissues (e.g., heart, liver) using differential centrifugation.
2.\tSetup Assay: Add isolated mitochondria (0.1-0.5 mg/mL) to a 96-well plate or spectrofluorometer cuvette containing respiratory buffer.
3.\tAdd Dye: Add Calcium Green-5N to a final concentration of 1 μM.
4.\tEnergize: Add substrate (e.g., 5 mM glutamate + 5 mM malate) and allow 2-5 min to incubate at room temperature, protecting from light.
5.\tInject Calcium: Program a plate reader to inject Cacl2 pulses (e.g., 5-10 μM per injection) every 1-2 minutes.
6.\tAnalyze: Monitor the increase in fluorescence. A sudden, sharp increase indicates the mitochondria are overloaded, the MPTP has opened, and is being released.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR outbred (male, cauda epididymides source)[1]
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Dosage:4 μM (loading concentration)
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Administration:passive intracellular loading; during final 30 minutes of sperm capacitation
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Result:Detected high levels of Ca2+ (≥ 1 μM) localized specifically to the acrosomal crescent and midpiece of acrosome-intact sperm, with no signal detected in the entire head or principal piece.
Lost signal in the acrosomal region when acrosomal exocytosis was induced with 20 μM thapsigargin.
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Chemical Information
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CAS No. 216699-33-1
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Appearance Oil
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Molecular Weight 1335.96
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Formula C59H52Cl2N4O282-
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Color Brown to reddish brown
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SMILES
O=C1OC2(C3=CC(Cl)=C(C=C3OC4=CC(OC(C)=O)=C(C=C42)Cl)OC(C)=O)C5=CC=C(C(NC6=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC7=CC([N-]([O-])=O)=CC=C7N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C6)=O)C=C15
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 6.68 mg/mL (5.00 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (288 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Herrick SB, et al. The acrosomal vesicle of mouse sperm is a calcium store. J Cell Physiol. 2005;202(3):663-671. [Content Brief]
[2]. Thomas D, et al. A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals. Cell Calcium. 2000;28(4):213-223. [Content Brief]
[3]. Rajdev S, et al. Calcium green-5N, a novel fluorescent probe for monitoring high intracellular free Ca2+ concentrations associated with glutamate excitotoxicity in cultured rat brain neurons. Neurosci Lett. 1993;162(1-2):149-152. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7485 mL | 3.7426 mL | 7.4853 mL | 18.7131 mL |
Calcium Green-5N AM Related Classifications
HY-D1631 Related Classifications
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