2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation Apoptosis
  3. Aldose Reductase Heme Oxygenase (HO) Reactive Oxygen Species (ROS) Apoptosis
  4. Soyasaponin Bb

Soyasaponin Bb  (Synonyms: ソヤサポニンI; ソーヤサポニンBb)

製品番号: HY-N0310 純度: 99.88%
COA 取扱説明書 Technical Support

Soyasaponin Bb is an orally active, covalent inducer of heme oxygenase HO-1 and an inhibitor of aldose reductase AKR1B1. Soyasaponin Bb can regulate oxidative stress pathways, enhance antioxidant capacity, reduce reactive oxygen species (ROS) generation, and inhibit lipid peroxidation and hepatocyte apoptosis. Soyasaponin Bb improves alcohol-induced hepatocyte membrane damage and liver function abnormalities, and improves Scopolamine (HY-N0296)-induced memory impairment. Soyasaponin Bb has antioxidant, hepatoprotective, and neuroprotective activities.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Soyasaponin Bb

Soyasaponin Bb 構造式

CAS 番号 : 51330-27-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 247 在庫あり
Solution
10 mM * 1 mL in DMSO USD 247 在庫あり
Solid
5 mg $119 在庫あり
10 mg $179 在庫あり
20 mg $267 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 1 publication(s) in Google Scholar

Other Forms of Soyasaponin Bb:

Soyasaponin Bb 関連抗体

Top Publications Citing Use of Products

Heme Oxygenase (HO) アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
HO-1 HO-2

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

Soyasaponin Bb is an orally active, covalent inducer of heme oxygenase HO-1 and an inhibitor of aldose reductase AKR1B1. Soyasaponin Bb can regulate oxidative stress pathways, enhance antioxidant capacity, reduce reactive oxygen species (ROS) generation, and inhibit lipid peroxidation and hepatocyte apoptosis. Soyasaponin Bb improves alcohol-induced hepatocyte membrane damage and liver function abnormalities, and improves Scopolamine (HY-N0296)-induced memory impairment. Soyasaponin Bb has antioxidant, hepatoprotective, and neuroprotective activities[1][2][3][4].

IC50 & Target

HO-1

 

体外実験

Soyasaponin Bb (25-100 μg/mL; pretreatment for 24 h + ethanol treatment for 24 h) increases cell viability, reduces reactive oxygen species (ROS) levels, increases superoxide dismutase (SOD) and glutathione (GSH) activities, downregulates malondialdehyde (MDA) and alanine aminotransferase (ALT) levels, and induces heme oxygenase-1 (HO-1) expression in rat hepatocyte BRL 3A assay[1].
Soyasaponin Bb (2.36-94.30 mg/L; 4 h) shows no significant cytotoxicity in the Caco-2 intestinal epithelial cell experiment. Soyasaponin Bb has an apparent permeability (Papp) across the membrane of less than 1.0×10-6 cm/s), with the apical-to-basolateral (AP-BL) transport significantly higher than the basolateral-to-apical (BL-AP) transport, indicating that the cell permeation of Soyasaponin Bb is mainly passive transport[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Soyasaponin Bb 関連抗体

Cell Viability Assay[1]

Cell Line: Rat BRL 3A hepatocytes
Concentration: 0, 25, 50, 100 μg/mL
Incubation Time: 24 h pretreatment with Soyasaponin Bb, followed by 24 h ethanol (250 mmol/L) treatment
Result: Dose-dependently increased cell viability, with 50 and 100 μg/mL groups showing significant protection compared to the ethanol-alone group.
The 25 μg/mL group showed minimal improvement.
体内実験

Soyasaponin Bb (100 mg/kg; oral administration; single dose) showes a peak plasma drug concentration of 19.8 ng/mL in the Sprague-Dawley rat model. It was mainly excreted in the feces, with very little excretion in the urine. The metabolite Soyasapogenol B was only detected in the feces[2].
Soyasaponin Bb (10 mg/kg; oral administration; once a day; for 5 consecutive days) significantly improves passive avoidance, Y-maze and Morris water maze task performance in the Scopolamine (HY-N0296)-induced mouse memory impairment model, increases brain-derived neurotrophic factor (BDNF) expression and cyclic adenosine monophosphate response element binding protein (CREB) phosphorylation level, without inhibiting acetylcholinesterase (AChE) activity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Scopolamine-induced ICR mice Memory Impairment Model (male; 18-22 g, 5 weeks old)[3]
Dosage: 10 mg/kg
Administration: Oral gavage, once 1 hour before scopolamine injection, single dose
Result: Significantly increased the latency time to enter the dark compartment in the retention trial, restoring memory impairment to 97% of the untreated normal control level, comparable to the positive control tacrine (10 mg/kg).
No significant differences were observed during the acquisition trial.
分子量

943.12

分子式

C48H78O18
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@H]1O[C@@]2([H])[C@H](O[C@]3([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)[C@H](O)[C@@H](C(O)=O)O[C@@]1([H])O[C@@H]4[C@@](CO)(C)[C@]5([H])CC[C@@]6(C)[C@]7(C)CC[C@@]8(C)[C@H](O)CC(C)(C)C[C@@]8([H])C7=CC[C@]6([H])[C@@]5(C)CC4
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (106.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0603 mL 5.3016 mL 10.6031 mL
5 mM 0.2121 mL 1.0603 mL 2.1206 mL
10 mM 0.1060 mL 0.5302 mL 1.0603 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (2.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.88%

COA (258 KB) HNMR (92 KB) RP-HPLC (208 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0603 mL 5.3016 mL 10.6031 mL 26.5078 mL
5 mM 0.2121 mL 1.0603 mL 2.1206 mL 5.3016 mL
10 mM 0.1060 mL 0.5302 mL 1.0603 mL 2.6508 mL
15 mM 0.0707 mL 0.3534 mL 0.7069 mL 1.7672 mL
20 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3254 mL
25 mM 0.0424 mL 0.2121 mL 0.4241 mL 1.0603 mL
30 mM 0.0353 mL 0.1767 mL 0.3534 mL 0.8836 mL
40 mM 0.0265 mL 0.1325 mL 0.2651 mL 0.6627 mL
50 mM 0.0212 mL 0.1060 mL 0.2121 mL 0.5302 mL
60 mM 0.0177 mL 0.0884 mL 0.1767 mL 0.4418 mL
80 mM 0.0133 mL 0.0663 mL 0.1325 mL 0.3313 mL
100 mM 0.0106 mL 0.0530 mL 0.1060 mL 0.2651 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Soyasaponin Bb Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Soyasaponin Bb51330-27-9Aldose ReductaseHeme Oxygenase (HO)Reactive Oxygen Species (ROS)ApoptosisHeme OxygenaseInhibitorinhibitorinhibit

最近チェックした製品:

オンラインお問い合わせ

Your information is safe with us. * Required Fields.

製品名

  

カスタマ需要量 *

お名前 *

 

タイトル

メールアドレス *

 

電話番号 *

デパートメント

 

組纖名 *

市区町村

都道府県

国或いは地域 *

      

必ず会社名を記載ください。個人への返信は行いません。

備考

バルクお問い合わせ

Inquiry Information

製品名:
Soyasaponin Bb
製品番号:
HY-N0310
数量:
MCE 日本正規代理店:

カスタマーレビュー

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

製品名

  

Lot #

お名前 *

 

メールアドレス *

電話番号 *

 

部門 *

組纖名 *

 

国或いは地域 *

備考

Request for HNMR Report

We have received your request and will respond to you as soon as possible.