2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. TCID

TCID  (Synonyms: 4,5,6,7-Tetrachloroindan-1,3-dione)

Cat. No.: HY-18638 Pureté: 99.78%
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TCID (4,5,6,7-Tetrachloroindan-1,3-dione) is a potent and selective neuronal ubiquitin C-terminal hydrolase (UCH-L3) inhibitor with an IC50 of 0.6 μM. TCID diminishes glycine transporter GlyT2 ubiquitination in brainstem and spinal cord primary neurons.

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CAS No. : 30675-13-9

Size Prix Stock Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
1 mg En stock
5 mg En stock
10 mg En stock
25 mg En stock
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100 mg   Obtenir un devis  

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Based on 6 publication(s) in Google Scholar

TCID Related Antibodies

Top Publications Citing Use of Products

    TCID purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 14;21(1):88.  [Abstract]

    qRT‒PCR of the mRNA expression of osteogenesis genes in BMSCs after treatment with UCHL3 inhibitor TCID (10 μM), and DMSO as a control.

    TCID purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 14;21(1):88.  [Abstract]

    Western blotting of RUNX2 and UCHL3 protein levels in BMSCs after treatment with UCHL3 inhibitor TCID (10 μM), and DMSO as a control.

    TCID purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 14;21(1):88.  [Abstract]

    ALP staining and Alizarin red staining in BMSCs after treatment with TCID (10 μM), and DMSO as a control.

    Voir tous les produits spécifiques à Isoform Deubiquitinase:

    Voir toutes les isoformes
    USP1 USP2 USP4 USP7 USP8 USP10 USP21 USP30 UCHL1 USP11 USP15 USP28 USP25

    • Activité biologique

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    Description

    TCID (4,5,6,7-Tetrachloroindan-1,3-dione) is a potent and selective neuronal ubiquitin C-terminal hydrolase (UCH-L3) inhibitor with an IC50 of 0.6 μM[1]. TCID diminishes glycine transporter GlyT2 ubiquitination in brainstem and spinal cord primary neurons[2].

    IC50 & Target

    IC50: 0.6 μM (UCH-L3) and 75 μM (UCH-L1)[1]
    In Vitro

    TCID (4,5,6,7-Tetrachloroindan-1,3-dione; compound 11) inhibits UCH-L1 with an IC50 of 75 μM[1].
    TCID (10 μM; 1, 2 hours) diminishes glycine transporter GlyT2 ubiquitination in brainstem and spinal cord primary neurons[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TCID Related Antibodies
    Masse moléculaire

    283.92

    Formule

    C9H2Cl4O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1CC(C2=C1C(Cl)=C(Cl)C(Cl)=C2Cl)=O
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvant et solubilité
    In Vitro: 

    DMSO : 20 mg/mL (70.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5221 mL 17.6106 mL 35.2212 mL
    5 mM 0.7044 mL 3.5221 mL 7.0442 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.33 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureté et documentation
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.5221 mL 17.6106 mL 35.2212 mL 88.0530 mL
    5 mM 0.7044 mL 3.5221 mL 7.0442 mL 17.6106 mL
    10 mM 0.3522 mL 1.7611 mL 3.5221 mL 8.8053 mL
    15 mM 0.2348 mL 1.1740 mL 2.3481 mL 5.8702 mL
    20 mM 0.1761 mL 0.8805 mL 1.7611 mL 4.4026 mL
    25 mM 0.1409 mL 0.7044 mL 1.4088 mL 3.5221 mL
    30 mM 0.1174 mL 0.5870 mL 1.1740 mL 2.9351 mL
    40 mM 0.0881 mL 0.4403 mL 0.8805 mL 2.2013 mL
    50 mM 0.0704 mL 0.3522 mL 0.7044 mL 1.7611 mL
    60 mM 0.0587 mL 0.2935 mL 0.5870 mL 1.4675 mL
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    TCID Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TCID30675-13-94,5,6,7-Tetrachloroindan-1,3-dioneDeubiquitinaseDUBsNeuronalubiquitinC-terminalhydrolaseUCH-L3UCH-L1glycinetransporterGlyT2ubiquitinationInhibitorinhibitorinhibit

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