2. Metabolic Enzyme/Protease
  3. Liposome Endogenous Metabolite
  4. 1,2-Dioleoyl-sn-glycero-3-phosphocholine

1,2-Dioleoyl-sn-glycero-3-phosphocholine  (Synonyms: DOPC)

Cat. No.: HY-113424A Pureza: 99.93%
COA
SDS
Instrucciones de manejo Technical Support

1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) is a phospholipid and is commonly used alone, or with other components, in the generation of micelles, liposomes, and other types of artificial membranes.

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1,2-Dioleoyl-sn-glycero-3-phosphocholine

1,2-Dioleoyl-sn-glycero-3-phosphocholine Estructura química

No. CAS : 4235-95-4

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Based on 10 publication(s) in Google Scholar

Other Forms of 1,2-Dioleoyl-sn-glycero-3-phosphocholine:

1,2-Dioleoyl-sn-glycero-3-phosphocholine Related Antibodies

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Descripciòn

1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) is a phospholipid and is commonly used alone, or with other components, in the generation of micelles, liposomes, and other types of artificial membranes.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Application of DOPC in biochemical experiments
1. Liposome construction
Drug delivery carrier: DOPC is widely used in liposome preparation due to its high membrane stability and low cytotoxicity. For example, liposomes formed by mixing DOPC and cholesterol (2:1 molar ratio) can efficiently encapsulate hydrophobic drugs (such as paclitaxel) with a drug loading efficiency of more than 85%, and achieve tumor-targeted delivery through enhanced permeability retention (EPR)[1].
Gene transfection assistance: DOPC is combined with cationic lipids (such as DOTAP) to form lipid complexes, which can reduce the cytotoxicity of cationic lipids and improve the transfection efficiency of plasmid DNA or siRNA[2].
2. Membrane protein research
Membrane protein reconstitution: DOPC is a commonly used phospholipid for the construction of nanodiscs. Together with membrane scaffold protein (MSP), it forms a phospholipid bilayer structure with a diameter of about 10 nm, providing a natural membrane environment for membrane proteins (such as GPCRs) to maintain their conformation and function[3].
Electrophysiological studies: DOPC is used to form a planar lipid bilayer (BLM), and combined with the patch clamp technique, the conductivity characteristics and gating mechanism of ion channels (such as potassium channels) are studied[4].
3. Cell membrane structral studies
Membrane fluidity analysis: DOPC lipid bilayers are labeled with fluorescent probes (such as DPH) to measure their microdomain fluidity and quantify the effect of cholesterol on membrane order (for every 10 mol% increase in cholesterol concentration, membrane fluidity decreases by 15%)[5].
Molecular interactions: Using surface plasmon resonance (SPR) technology, the affinity of the antimicrobial peptide LL-37 to DOPC membranes was measured (KD ≈ 1.2×10⁻⁶ M), revealing its membrane damage mechanism[6].
4. Cell toxicity assessment
Toxicity control: As a neutral phospholipid, DOPC is often used as a negative control to evaluate the cytotoxicity of other lipid nanoparticles (e.g., cationic liposomes)[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1,2-Dioleoyl-sn-glycero-3-phosphocholine Related Antibodies
Peso molecular

786.11

Fòrmula

C44H84NO8P
No. CAS
Appearance

<-21 °C Solid,>-21 °C Liquid

Color

Colorless to off-white

SMILES

CCCCCCCC/C=C\CCCCCCCC(OC[C@@H](OC(CCCCCCC/C=C\CCCCCCCC)=O)COP(OCC[N+](C)(C)C)([O-])=O)=O
Structure Classification
Initial Source
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvente y solubilidad
In Vitro: 

Ethanol : 50 mg/mL (63.60 mM; Need ultrasonic)

DMSO : 5 mg/mL (6.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2721 mL 6.3604 mL 12.7209 mL
5 mM 0.2544 mL 1.2721 mL 2.5442 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 0.5 mg/mL (0.64 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 0.5 mg/mL (0.64 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación

Purity: 99.93%

SDS (647 KB)

COA (240 KB) HNMR (292 KB) RP-HPLC (210 KB) MS (87 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / Ethanol 1 mM 1.2721 mL 6.3604 mL 12.7209 mL 31.8022 mL
5 mM 0.2544 mL 1.2721 mL 2.5442 mL 6.3604 mL
Ethanol 10 mM 0.1272 mL 0.6360 mL 1.2721 mL 3.1802 mL
15 mM 0.0848 mL 0.4240 mL 0.8481 mL 2.1201 mL
20 mM 0.0636 mL 0.3180 mL 0.6360 mL 1.5901 mL
25 mM 0.0509 mL 0.2544 mL 0.5088 mL 1.2721 mL
30 mM 0.0424 mL 0.2120 mL 0.4240 mL 1.0601 mL
40 mM 0.0318 mL 0.1590 mL 0.3180 mL 0.7951 mL
50 mM 0.0254 mL 0.1272 mL 0.2544 mL 0.6360 mL
60 mM 0.0212 mL 0.1060 mL 0.2120 mL 0.5300 mL
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1,2-Dioleoyl-sn-glycero-3-phosphocholine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1,2-Dioleoyl-sn-glycero-3-phosphocholine4235-95-4DOPCLiposomeEndogenous MetaboliteInhibitorinhibitorinhibit

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Nombre del producto:
1,2-Dioleoyl-sn-glycero-3-phosphocholine
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HY-113424A
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