2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. 2'-Deoxycytidine

Deoxycytidine  (Synonyms: 2'-Deoxycytidine; 2′-デオキシシチジン; Cytosine deoxyriboside; Deoxyribose cytidine)

製品番号: HY-D0184 純度: 99.95%
COA 取扱説明書 Technical Support

2'-Deoxycytidine, a deoxyribonucleoside, can inhibit biological effects of Bromodeoxyuridine (Brdu). 2'-Deoxycytidine is essential for the synthesis of nucleic acids, that can be used for the research of cancer.

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2'-Deoxycytidine

Deoxycytidine 構造式

CAS 番号 : 951-77-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
500 mg $25 在庫あり
1 g $40 在庫あり
5 g $120 在庫あり
10 g $192 在庫あり
25 g $385 在庫あり
50 g $616 在庫あり
> 100 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 9 publication(s) in Google Scholar

Other Forms of 2'-Deoxycytidine:

Deoxycytidine 関連抗体

Top Publications Citing Use of Products

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Human Endogenous Metabolite Microbial Metabolite

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製品説明

2'-Deoxycytidine, a deoxyribonucleoside, can inhibit biological effects of Bromodeoxyuridine (Brdu). 2'-Deoxycytidine is essential for the synthesis of nucleic acids, that can be used for the research of cancer[1][2].

IC50 & Target[1]

Brdu

 

Human Endogenous Metabolite

 

Microbial Metabolite

 

Cellular Effect
Cell Line Type Value Description References
C6 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
ATH-8 cell line ED50
>100 3
Compound: 4b
Protection of ATH8 cells against the cytopathic effect of HIV.
Protection of ATH8 cells against the cytopathic effect of HIV.
[PMID: 3497272]
C6 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
DU-145 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
C6 IC50
>250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
HaCaT IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
DU-145 IC50
>250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
DU-145 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
HCT-116 EC50
> 10 3
Compound: 5azadC
Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay
Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay
[PMID: 26220519]
HCT-116 EC50
> 10 3
Compound: 5azadC
Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay
Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay
[PMID: 26220519]
HaCaT IC50
>250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
KARPAS-299 EC50
> 0 3
Compound: 5azadC
Antiproliferative activity against human KARPAS299 cells after 2 to 4 days by ATPlite 1step luminescence assay
Antiproliferative activity against human KARPAS299 cells after 2 to 4 days by ATPlite 1step luminescence assay
[PMID: 26220519]
HCT-116 EC50
>10 3
Compound: 5azadC
Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay
Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay
[PMID: 26220519]
HaCaT IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
KG-1 EC50
0.1 3
Compound: 5azadC
Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay
Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay
[PMID: 26220519]
KG-1 EC50
0.1 3
Compound: 5azadC
Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay
Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay
[PMID: 26220519]
L6 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
L6 IC50
>250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
MCF7 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
MCF7 IC50
>250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
KG-1 EC50
0.1 3
Compound: 5azadC
Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay
Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay
[PMID: 26220519]
L6 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
MCF7 IC50
> 250 3
Compound: 2'-Deoxycytidine
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
体内実験

2'-Deoxycytidine (25 mg/kg, i.v., 4 times, from 8 days to 16 days) inhibits tumor growth in mice inoculated SP2/0 myeloma cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice inoculated SP2/0 myeloma cells[2]
Dosage: 25 mg/kg
Administration: i.v., 4 times, from 8 days to 16 days
Result: Decreased the tumor weight and volume.
Increased survival time.
臨床実験
分子量

227.22

分子式

C9H13N3O4
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C1N([C@H]2C[C@H](O)[C@@H](CO)O2)C=CC(N)=N1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

H2O : 100 mg/mL (440.10 mM; Need ultrasonic)

DMSO : 50 mg/mL (220.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4010 mL 22.0051 mL 44.0102 mL
5 mM 0.8802 mL 4.4010 mL 8.8020 mL
10 mM 0.4401 mL 2.2005 mL 4.4010 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 110 mg/mL (484.11 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.95%

COA (251 KB) RP-HPLC (244 KB) MS (68 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.4010 mL 22.0051 mL 44.0102 mL 110.0255 mL
5 mM 0.8802 mL 4.4010 mL 8.8020 mL 22.0051 mL
10 mM 0.4401 mL 2.2005 mL 4.4010 mL 11.0026 mL
15 mM 0.2934 mL 1.4670 mL 2.9340 mL 7.3350 mL
20 mM 0.2201 mL 1.1003 mL 2.2005 mL 5.5013 mL
25 mM 0.1760 mL 0.8802 mL 1.7604 mL 4.4010 mL
30 mM 0.1467 mL 0.7335 mL 1.4670 mL 3.6675 mL
40 mM 0.1100 mL 0.5501 mL 1.1003 mL 2.7506 mL
50 mM 0.0880 mL 0.4401 mL 0.8802 mL 2.2005 mL
60 mM 0.0734 mL 0.3668 mL 0.7335 mL 1.8338 mL
80 mM 0.0550 mL 0.2751 mL 0.5501 mL 1.3753 mL
100 mM 0.0440 mL 0.2201 mL 0.4401 mL 1.1003 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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2'-Deoxycytidine Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2'-Deoxycytidine951-77-9Deoxycytidine Cytosine deoxyriboside Deoxyribose cytidineEndogenous Metabolitedeoxyribonucleosidecancernucleic acidsBrduInhibitorinhibitorinhibit

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製品名:
2'-Deoxycytidine
製品番号:
HY-D0184
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