Cat. No.: HY-17564

2'-Deoxycytidine (hydrochloride)

Name: 2'-Deoxycytidine (hydrochloride)
Brand: MedChemExpress
SKU: HY-17564
Price: 25.00 USD
Availability: InStock
Rating: 5 (9 reviews)

(Synonyms: 2'-Deoxycytidine monohydrochloride; Deoxycytidine hydrochloride; NSC 83251)

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

2'-Deoxycytidine hydrochloride (2'-Deoxycytidine monohydrochloride; NSC 83251) is an orally active nucleic acid biosynthesis enzyme inhibitor. 2'-Deoxycytidine hydrochloride competes with nucleic acid biosynthesis enzymes and nucleoside transporters, thereby reducing the toxicity of cytarabine. 2'-Deoxycytidine hydrochloride can be used in the research of L1210 lymphoblastic leukemia.

For research use only. We do not sell to patients.

Purity: 99.98%
CAS No.: 3992-42-5
Formula: C₉H₁₄ClN₃O₄
Molecular Weight:263.68

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

2'-Deoxycytidine (hydrochloride) Chemical Structure

CAS No.: 3992-42-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vivo
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vivo

2'-Deoxycytidine (40-480 mg/kg; p.o.; three/four times daily; 4-18 days) hydrochloride co-administration completely prevents araC-induced lethal toxicity in normal mice, partially or fully protects most hematopoietic cell lineages except bone marrow lymphocytes, and normalizes araC-induced cellular immune suppression without reversing araC's humoral immune suppression^[1].
2'-Deoxycytidine (360-480 mg/kg; p.o.; three/four times daily; days 5-26) hydrochloride co-administration prevents araC-induced lethal toxicity in leukemia-bearing mice, maintains or enhances araC's antitumor efficacy against L1210 lymphoblastic leukemia, and reduces araC's antitumor effect against non-lymphoid Graffi MG and Rauscher ER leukemias^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6j (B6) female; DBA/2 (D2) female; outbred white; (DBA/2 x C57BL/6j) F1 (B6D2F1) male; BALB/c (C) female^[1]
Dosage:

40 mg/kg; 120 mg/kg; 180 mg/kg; 360 mg/kg; 480 mg/kg; 48 mg/kg
Administration:

p.o.; three times daily (4 days, days 5-7, 11-13, 18-20); four times daily (5 days, days 5-7, 12-14, 19-20, days 8,9,13-15, days 10-18, days 10-17 then escalated to 480 mg/kg four times daily on days 13-18)
Result:

Prevented araC-induced lethal toxicity.
Reduced araC-induced diarrhea.
Resulted in significantly higher peripheral blood leukocyte, lymphocyte, and granulocyte counts compared to araC-only treated mice at 3-4 days post-treatment.
Completely protected bone marrow reticular cells, hemocytoblasts, mitotic granulocytes, and total mitotic cells.
Partially protected bone marrow postmitotic granulocytes, erythroid cells, and total nucleated cells.
Did not protect bone marrow lymphocytes.

Chemical Information

CAS No. 3992-42-5
Appearance Solid
Molecular Weight 263.68
Formula C₉H₁₄ClN₃O₄
Color White to off-white
SMILES

OC[C@@H]1[C@@H](O)C[C@H](N2C(N=C(N)C=C2)=O)O1.Cl
Synonyms

2'-Deoxycytidine monohydrochloride; Deoxycytidine hydrochloride; NSC 83251
Structure Classification
- Others
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (9)

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Int J Biol Macromol

Crude polysaccharide from Danggui Buxue decoction enhanced the anti-tumor effect of gemcitabine by remodeling tumor-associated macrophages. [Abstract]

2023 Jul 1;242(Pt 4):125063. PMID: 37245770
Cell Rep

SAMHD1 Limits the Efficacy of Forodesine in Leukemia by Protecting Cells against the Cytotoxicity of dGTP. [Abstract]

2020 May 12;31(6):107640. PMID: 32402273
Anal Chem

Ultrahigh Flow Regulated Discharge Coupling with Targeted Mass Spectrometry Analysis: Real Time Capturing Biomolecules in Exhaled Breath. [Abstract]

2025 May 13;97(18):9827-9835. PMID: 40302637
JCI Insight

Augmenting chemotherapy with low-dose decitabine through an immune-independent mechanism. [Abstract]

2022 Nov 22;7(22):e159419. PMID: 36227698
Food Biosci

2025 Jun.
Clin Epigenetics

Methylation silencing of TGF-β receptor type II is involved in malignant transformation of esophageal squamous cell carcinoma. [Abstract]

2020 Feb 11;12(1):25. PMID: 32046777
J Pharm Biomed Anal

A novel UHPLC-MS/MS approach for simultaneous quantification of pyrimidine metabolites in human biofluids. [Abstract]

2025 Nov 15:265:117026. PMID: 40544701
Research Square Preprint

2021 May.

Solvent & Solubility

In Vitro:

H₂O : ≥ 41 mg/mL (155.49 mM)

DMSO : 25 mg/mL (94.81 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.7925 mL

18.9624 mL

37.9248 mL

5 mM

0.7585 mL

3.7925 mL

7.5850 mL

10 mM

0.3792 mL

1.8962 mL

3.7925 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (9.48 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (9.48 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (189.62 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. V. M. Buchman, et al. 2′-Deoxycytidine hydrochloride protection of mice against the lethal toxicity of cytosine arabinoside. Cancer Chemotherapy and Pharmacology. Volume 3, pages 229–234, (1979).

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	3.7925 mL	18.9624 mL	37.9248 mL	94.8119 mL
	5 mM	0.7585 mL	3.7925 mL	7.5850 mL	18.9624 mL
	10 mM	0.3792 mL	1.8962 mL	3.7925 mL	9.4812 mL
	15 mM	0.2528 mL	1.2642 mL	2.5283 mL	6.3208 mL
	20 mM	0.1896 mL	0.9481 mL	1.8962 mL	4.7406 mL
	25 mM	0.1517 mL	0.7585 mL	1.5170 mL	3.7925 mL
	30 mM	0.1264 mL	0.6321 mL	1.2642 mL	3.1604 mL
	40 mM	0.0948 mL	0.4741 mL	0.9481 mL	2.3703 mL
	50 mM	0.0758 mL	0.3792 mL	0.7585 mL	1.8962 mL
	60 mM	0.0632 mL	0.3160 mL	0.6321 mL	1.5802 mL
	80 mM	0.0474 mL	0.2370 mL	0.4741 mL	1.1851 mL
H₂O	100 mM	0.0379 mL	0.1896 mL	0.3792 mL	0.9481 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

2'-Deoxycytidine (hydrochloride)

9 Cited Publications

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2'-Deoxycytidine (hydrochloride)

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