5-Fluoroorotic acid

Name: 5-Fluoroorotic acid
Brand: MedChemExpress
SKU: HY-W016819
Rating: 4.5 (0 reviews)

製品番号: HY-W016819 純度: 99.17%: Data Sheet SDS COA 取扱説明書
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5-Fluoroorotic acid is the inhibitor for thymidylate synthase that acts as a selective agent in yeast molecular genetics. 5-Fluoroorotic acid exhibits antimalarial activity.

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5-Fluoroorotic acid 構造式

CAS 番号 : 703-95-7

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solid
500 mg	$30	在庫あり	Estimated Time of Arrival: December 31
1 g	$50	在庫あり	Estimated Time of Arrival: December 31
5 g		お問い合わせ
10 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 0 publication(s) in Google Scholar

Other Forms of 5-Fluoroorotic acid:

5-Fluoroorotic acid (Standard) お問い合わせ

Parasite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

5-Fluoroorotic acid is the inhibitor for thymidylate synthase that acts as a selective agent in yeast molecular genetics. 5-Fluoroorotic acid exhibits antimalarial activity^[1]^[2]^[3].

体外実験

5-Fluoroorotic acid (1 mg/mL, 4-7 days) inhibits thymidylate synthase, selectively inhibits the growth of Ura+ cells with the presence of UMP synthase, and can be used for screening of Ura- mutated yeast (such as Saccharomyces cerevisiae)^[1]^[3].
5-Fluoroorotic acid (1 nM-100 μM, 48 h) exhibits antimalarial activity against Plasmodium falciparum with an IC₅₀ of 6.0 nM. 5-Fluoroorotic acid exhibits relatively lower cytotoxicity in mammalian cell HT-1080 with an IC₅₀ of 10 μM^[4]^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[4]

Cell Line:	HT-1080
Concentration:	1 nM-100 μM
Incubation Time:	48 h
Result:	Inhibited cell proliferation.

体内実験

5-Fluoroorotic acid (0.2-5 mg/kg, ip, every 4 hours for 3 days) suppresses malarial parasitemia, exhibits toxicity with the absence of uridine (at a dose of 5 mg/kg) in P. yoelii-infected mouse models^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	P. yoelii-infected mouse models^[5]
Dosage:	0.2-5 mg/kg
Administration:	ip, every 4 hours for 3 days
Result:	Eliminated malarial parasites. Caused weight loss, diarrhea, decreased numbers of leukocytes, and increased mortality (5 mg/kg).

分子量

174.09

分子式

C₅H₃FN₂O₄

CAS 番号

703-95-7

Appearance

Solid

Color

Light brown to brown

SMILES

O=C(C(NC1=O)=C(F)C(N1)=O)O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (574.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.7442 mL	28.7208 mL	57.4416 mL
5 mM	1.1488 mL	5.7442 mL	11.4883 mL
10 mM	0.5744 mL	2.8721 mL	5.7442 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (11.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.84%

Select Batch:

データシート (276 KB) SDS (396 KB)

COA (250 KB) HNMR (252 KB) LCMS (92 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Boeke JD, et, al. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 1987; 154: 164-75. [Content Brief]

[2]. Nikolova K, et, al. Studies on the antimycotic activity of 5-fluoroorotic acid. Methods Find Exp Clin Pharmacol. 1987 Feb; 9(2): 85-9. [Content Brief]

[3]. Santoso D, et al., Fluoroorotic acid-selected Nicotiana plumbaginifolia cell lines with a stable thymine starvation phenotype have lost the thymine-regulated transcriptional program. Plant Physiol. 2000 Aug;123(4):1517-24. [Content Brief]

[4]. Rathod PK, et al., Selective activity of 5-fluoroorotic acid against Plasmodium falciparum in vitro. Antimicrob Agents Chemother. 1989 Jul;33(7):1090-4. [Content Brief]

[5]. Gómez ZM, et al., Antimalarial activity of a combination of 5-fluoroorotate and uridine in mice. Antimicrob Agents Chemother. 1990 Jul;34(7):1371-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.7442 mL	28.7208 mL	57.4416 mL	143.6039 mL
	5 mM	1.1488 mL	5.7442 mL	11.4883 mL	28.7208 mL
	10 mM	0.5744 mL	2.8721 mL	5.7442 mL	14.3604 mL
	15 mM	0.3829 mL	1.9147 mL	3.8294 mL	9.5736 mL
	20 mM	0.2872 mL	1.4360 mL	2.8721 mL	7.1802 mL
	25 mM	0.2298 mL	1.1488 mL	2.2977 mL	5.7442 mL
	30 mM	0.1915 mL	0.9574 mL	1.9147 mL	4.7868 mL
	40 mM	0.1436 mL	0.7180 mL	1.4360 mL	3.5901 mL
	50 mM	0.1149 mL	0.5744 mL	1.1488 mL	2.8721 mL
	60 mM	0.0957 mL	0.4787 mL	0.9574 mL	2.3934 mL
	80 mM	0.0718 mL	0.3590 mL	0.7180 mL	1.7950 mL
	100 mM	0.0574 mL	0.2872 mL	0.5744 mL	1.4360 mL