2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. 5-Hydroxymethylcytosine

5-Hydroxymethylcytosine  (Synonyms: 5hmC)

製品番号: HY-W018324 純度: 98.14%
COA 取扱説明書 Technical Support

5-Hydroxymethylcytosine (5hmC) is an oxidized forms of 5-methylcytosine (5mC) in mammalian DNA. 5-Hydroxymethylcytosine is produced from 5mC in an enzymatic pathway involving three 5mC oxidases, Ten-eleven translocation (TET)1, TET2, and TET3. The conversion of 5mC into 5hmC can be the first step in a pathway leading towards DNA demethylation. 5-Hydroxymethylcytosine is associated with gene transcription and frequently used as a mark to investigate dynamic DNA methylation conversion during mammalian development. 5-Hydroxymethylcytosine can be used for the study of non-small cell lung cancer (NSCLC), neurodegenerative diseases (Alzheimer’s, Parkinson’s) and hematological malignancies (acute myeloid leukemia, myelodysplastic syndromes).

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5-Hydroxymethylcytosine

5-Hydroxymethylcytosine 構造式

CAS 番号 : 1123-95-1

容量 価格（税別） 在庫状況 数量
5 mg $112 在庫あり
10 mg $168 在庫あり
25 mg $340 在庫あり
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100 mg   お問い合わせ  

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Other Forms of 5-Hydroxymethylcytosine:

5-Hydroxymethylcytosine 関連抗体

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製品説明

5-Hydroxymethylcytosine (5hmC) is an oxidized forms of 5-methylcytosine (5mC) in mammalian DNA. 5-Hydroxymethylcytosine is produced from 5mC in an enzymatic pathway involving three 5mC oxidases, Ten-eleven translocation (TET)1, TET2, and TET3. The conversion of 5mC into 5hmC can be the first step in a pathway leading towards DNA demethylation. 5-Hydroxymethylcytosine is associated with gene transcription and frequently used as a mark to investigate dynamic DNA methylation conversion during mammalian development. 5-Hydroxymethylcytosine can be used for the study of non-small cell lung cancer (NSCLC), neurodegenerative diseases (Alzheimer’s, Parkinson’s) and hematological malignancies (acute myeloid leukemia, myelodysplastic syndromes)[1][2].

体外実験

5-Hydroxymethylcytosine (5hmC) is a stable epigenetic modification derived from the oxidation of 5-methylcytosine (5mC) by ten-eleven translocation (TET) enzymes (TET1, TET2, TET3). 5-Hydroxymethylcytosine functions as both an intermediate in active DNA demethylation and an independent regulator of gene expression-enriching in CpG islands, active/bivalent promoters, and transcriptional enhancers to promote euchromatin formation and modulate transcription factor binding. 5-Hydroxymethylcytosine plays pivotal roles in biological processes: maintaining embryonic stem cell (ESC) pluripotency, driving neurodevelopment, and restraining tumor progression[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-Hydroxymethylcytosine 関連抗体
臨床実験
分子量

141.13

分子式

C5H7N3O2
CAS 番号
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1N=CC(CO)=C(N1)N
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 6.25 mg/mL (44.29 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.0857 mL 35.4283 mL 70.8567 mL
5 mM 1.4171 mL 7.0857 mL 14.1713 mL
10 mM 0.7086 mL 3.5428 mL 7.0857 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

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体積 （開始）

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In Vivo Dissolution Calculator
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純度とドキュメンテーション

純度: 99.91%

COA (248 KB) RP-HPLC (283 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 7.0857 mL 35.4283 mL 70.8567 mL 177.1416 mL
5 mM 1.4171 mL 7.0857 mL 14.1713 mL 35.4283 mL
10 mM 0.7086 mL 3.5428 mL 7.0857 mL 17.7142 mL
15 mM 0.4724 mL 2.3619 mL 4.7238 mL 11.8094 mL
20 mM 0.3543 mL 1.7714 mL 3.5428 mL 8.8571 mL
25 mM 0.2834 mL 1.4171 mL 2.8343 mL 7.0857 mL
30 mM 0.2362 mL 1.1809 mL 2.3619 mL 5.9047 mL
40 mM 0.1771 mL 0.8857 mL 1.7714 mL 4.4285 mL
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5-Hydroxymethylcytosine Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
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Keywords:

5-Hydroxymethylcytosine1123-95-15hmCEndogenous Metabolite5-methylcytosine5mCmammalian5mC oxidasesTen-eleven translocationTET1TET2TET3DNA demethylationgene transcriptionInhibitorinhibitorinhibit

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5-Hydroxymethylcytosine
製品番号:
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