7-Ethoxyresorufin  (Synonyms: Resorufin ethyl ether)

7-Ethoxyresorufin (Resorufin ethyl ether) is a fluorometric substrate and competitive inhibitor of cytochrome P450, especially CYP1A1. 7-Ethoxyresorufin also inhibits NO synthase^[1][2][3][4].

7-Ethoxyresorufin (2 μM, 10-15 min) reduces the relaxant actions of acetylcholine (0.01-10 μM) in endothelium-intact rings and of NO (0.3-10 M), GTN (0.01-10 μM) and SNP (0.01-10 μM) in endothelium-denuded rings in concentration-dependent manners in rat aortic rings^[1].
7-Ethoxyresorufin (2.5, 5.0 and 10 μM, 2 h) reacts with NADPH in the presence of acid with the formation of a product which fluoresces at the same wavelength as Resorufin (HY-123533)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

241.24

C₁₄H₁₁NO₃

5725-91-7

Solid

Orange to red

460

335

O=C1C=CC2=NC3=C(C=C(OCC)C=C3)OC2=C1

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. C G Li, et al. Inhibition of NO-medicate responses by 7-ethoxyresorufin, a substrate and competitive inhibitor of cytochrome P450. Br J Pharmacol. 1996 May;118(1):57-62.  [Content Brief]

  [2]. Thomas K H Chang, et al. Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate. Methods Mol Biol. 2006;320:85-90.  [Content Brief]

  [3]. Grafstöm R, et al. Inhibitors of 7-ethoxyresorufin and 7-ethoxycoumarin de-ethylases in rat small intestinal microsomes and cells. Drug Chem Toxicol. 1981;4(2):147-59.  [Content Brief]

  [4]. Pohl RJ, et al. A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions. Anal Biochem. 1980 Sep 1;107(1):150-5.  [Content Brief]