1. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  2. Reactive Oxygen Species
  3. Resorufin

Resorufin  (Synonyms: NSC 12097)

Cat. No.: HY-123533 Purity: ≥99.0%
COA Handling Instructions

Resorufin (NSC 12097) is a highly fluorescent pink dye for the detection of ROS/RNS and a second analyte.

For research use only. We do not sell to patients.

Resorufin Chemical Structure

Resorufin Chemical Structure

CAS No. : 635-78-9

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Based on 1 publication(s) in Google Scholar

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Description

Resorufin (NSC 12097) is a highly fluorescent pink dye for the detection of ROS/RNS and a second analyte[1].

Molecular Weight

213.19

Formula

C12H7NO3

CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

584

Excitation (Ex)

571

SMILES

O=C1C=CC2=NC3=C(OC2=C1)C=C(C=C3)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

0.5 M NaOH : 5 mg/mL (23.45 mM; ultrasonic and warming and adjust pH to 12 with NaOH and heat to 60°C)

Ethanol : < 1 mg/mL (insoluble)

H2O : < 0.1 mg/mL (insoluble)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6907 mL 23.4533 mL 46.9065 mL
5 mM 0.9381 mL 4.6907 mL 9.3813 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
0.5 M NaOH 1 mM 4.6907 mL 23.4533 mL 46.9065 mL 117.2663 mL
5 mM 0.9381 mL 4.6907 mL 9.3813 mL 23.4533 mL
10 mM 0.4691 mL 2.3453 mL 4.6907 mL 11.7266 mL
15 mM 0.3127 mL 1.5636 mL 3.1271 mL 7.8178 mL
20 mM 0.2345 mL 1.1727 mL 2.3453 mL 5.8633 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Resorufin
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