Resorufin sodium salt
Based on 1 Customer Validation
Resorufin sodium salt (NSC 12097) is a highly fluorescent pink dye. Resorufin sodium salt shows favorable properties such as water solubility, red-shifted absorption/emission signals. Resorufin sodium salt is used for the detection of ROS/RNS and a second analyte.
For research use only. We do not sell to patients.
- Purity: 99.42%
- CAS No.: 34994-50-8
- Formula: C12H6NNaO3
- Molecular Weight:235.17
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
Preparation of Resorufin sodium salt staining solution
1.1 Preparation of stock solution
Prepare a 1000 μM Resorufin sodium salt stock solution in DMSO.
Note: (1) It is recommended to aliquot the Resorufin sodium salt stock solution and store at -20°C protected from light.
1.2 Preparation of working solution
Prepare a 0.4 μM Resorufin sodium salt working solution in PBS (pH 7.4).
Resorufin sodium salt staining
1. Pre-treat HepG2 cells with CD for 30 min, then remove the culture medium. After that, wash the cells three times with 200 μL PBS, and add 150 μL fresh DMEM and 15 μL of 0.4 μM Resorufin sodium salt solution (diluted in DMEM) to each well. Incubate at 37 °C in the dark for 30 min, then measure Resorufin content using a plate reader.
2. Pre-treat HepG2 cells with CD for 120 min, then remove the culture medium. After that, wash the cells three times with 200 μL PBS, and add 150 μL fresh DMEM and 15 μL of 0.4 μM Resorufin sodium salt solution (diluted in DMEM) to each well. Incubate at 37 °C in the dark for 120 min, then measure Resorufin content using a plate reader.
3. Measure fluorescence/absorbance and perform routine quantitative analysis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 34994-50-8
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Appearance Solid
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Molecular Weight 235.17
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Formula C12H6NNaO3
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Color Dark purple to black
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SMILES
O=C1C=CC2=NC3=C(OC2=C1)C=C(C=C3)O[Na]
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Synonyms
NSC 12097 sodium salt
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 62.5 mg/mL (265.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (21.26 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Odyniec ML , et al. 'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte. Chem Commun (Camb). 2018 Jul 26;54(61):8466-8469. [Content Brief]
[2]. Almammadov T, et al. Resorufin Enters the Photodynamic Therapy Arena: A Monoamine Oxidase Activatable Agent for Selective Cytotoxicity. ACS Med Chem Lett. 2020 Nov 23;11(12):2491-2496. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.2522 mL | 21.2612 mL | 42.5224 mL | 106.3061 mL |
| 5 mM | 0.8504 mL | 4.2522 mL | 8.5045 mL | 21.2612 mL | |
| 10 mM | 0.4252 mL | 2.1261 mL | 4.2522 mL | 10.6306 mL | |
| 15 mM | 0.2835 mL | 1.4174 mL | 2.8348 mL | 7.0871 mL | |
| 20 mM | 0.2126 mL | 1.0631 mL | 2.1261 mL | 5.3153 mL | |
| DMSO | 25 mM | 0.1701 mL | 0.8504 mL | 1.7009 mL | 4.2522 mL |
| 30 mM | 0.1417 mL | 0.7087 mL | 1.4174 mL | 3.5435 mL | |
| 40 mM | 0.1063 mL | 0.5315 mL | 1.0631 mL | 2.6577 mL | |
| 50 mM | 0.0850 mL | 0.4252 mL | 0.8504 mL | 2.1261 mL | |
| 60 mM | 0.0709 mL | 0.3544 mL | 0.7087 mL | 1.7718 mL | |
| 80 mM | 0.0532 mL | 0.2658 mL | 0.5315 mL | 1.3288 mL | |
| 100 mM | 0.0425 mL | 0.2126 mL | 0.4252 mL | 1.0631 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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