2. Apoptosis Metabolic Enzyme/Protease
  3. MDM-2/p53 HIF/HIF Prolyl-Hydroxylase
  4. Amifostine

Amifostine  (Synonyms: WR2721; アミホスチン)

製品番号: HY-B0639 純度: 99.95%
COA 取扱説明書 Technical Support

Amifostine (WR2721) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine is potent hypoxia-inducible factor-α1 (HIF-α1) and p53 inducer. Amifostine protects cells from damage by scavenging oxygen-derived free radicals. Amifostine reduces renal toxicity and has antiangiogenic action.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 20537-88-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 45 在庫あり
Solution
10 mM * 1 mL in Water USD 45 在庫あり
Solid
5 mg $41 在庫あり
10 mg $65 在庫あり
25 mg $110 在庫あり
50 mg $154 在庫あり
100 mg $215 在庫あり
200 mg $300 在庫あり
500 mg   お問い合わせ  
1 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 13 publication(s) in Google Scholar

Other Forms of Amifostine:

Amifostine 関連抗体

Top Publications Citing Use of Products

    Amifostine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Feb 13;6(2):e70092.  [Abstract]

    The dynamics of the numbers of CFUs in the bone marrow from indicated groups within a month post‐irradiation. The results showed that the reduction in colony-forming unit (CFU) counts in mice treated with Amifostine (150 mg/kg; i.p.; single dose) was relatively mild at 7 days post-irradiation.

    Amifostine purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58.  [Abstract]

    HE staining of testis among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

    Amifostine purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58.  [Abstract]

    Protein level alterations of Bclaf1, P-Rela, Rela, Sycp3, and β-actin were analyzed among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

    Amifostine purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58.  [Abstract]

    RT-qPCR analysis of Spliceosome-related gene expression levels across the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

    Amifostine purchased from MedChemExpress. Usage Cited in: Bioact Mater.2024 May 21:39:191-205.  [Abstract]

    Cell viabilities of A31 cells under different doses of IR (0, 2, 4, 6, 8, and 10 Gy) with pretreatment of tFNAs, Amifostine (AMF, 54 μg/mL; 12 h), or nanosuit (250 nM; 12 h) (n = 5).

    HIF/HIF Prolyl-Hydroxylase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    HIF-1α HIF

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Amifostine (WR2721) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine is potent hypoxia-inducible factor-α1 (HIF-α1) and p53 inducer. Amifostine protects cells from damage by scavenging oxygen-derived free radicals. Amifostine reduces renal toxicity and has antiangiogenic action[1][2][3][4].

    体外実験

    Amifostine (0.78125-100 μM, 24 h) reduces tert-Butyl hydroperoxide (TBHP)-induced cell damage in a dose-dependent manner and significantly reduces H9c2 cells apoptosis at a concentration of 100 μM[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Amifostine 関連抗体
    体内実験

    Amifostine (i.v., 400 mg/kg, 4 h) has a protective effect against myocardial I/R injury in male C57BL/6 mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/6 mice with myocardial I/R injury[5]
    Dosage: 400 mg/kg
    Administration: Intravenous injection; 4 hours
    Result: Attenuated cardiomyocyte apoptosis and reduced the production of I/R-induced ROS.
    Significantly reduced the expression of cleaved caspase 3 and Bax while enhanced the expression of SOD1, SOD2 and Bcl2.
    Significantly increased SOD activity and reduced MDA levels.
    臨床実験
    分子量

    214.22

    分子式

    C5H15N2O3PS
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OP(SCCNCCCN)(O)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    H2O : 100 mg/mL (466.81 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.6681 mL 23.3405 mL 46.6810 mL
    5 mM 0.9336 mL 4.6681 mL 9.3362 mL
    10 mM 0.4668 mL 2.3340 mL 4.6681 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

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    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (466.81 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.95%

    COA (242 KB) HNMR (213 KB) CNMR (219 KB) RP-HPLC (167 KB) MS (85 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 4.6681 mL 23.3405 mL 46.6810 mL 116.7025 mL
    5 mM 0.9336 mL 4.6681 mL 9.3362 mL 23.3405 mL
    10 mM 0.4668 mL 2.3340 mL 4.6681 mL 11.6702 mL
    15 mM 0.3112 mL 1.5560 mL 3.1121 mL 7.7802 mL
    20 mM 0.2334 mL 1.1670 mL 2.3340 mL 5.8351 mL
    25 mM 0.1867 mL 0.9336 mL 1.8672 mL 4.6681 mL
    30 mM 0.1556 mL 0.7780 mL 1.5560 mL 3.8901 mL
    40 mM 0.1167 mL 0.5835 mL 1.1670 mL 2.9176 mL
    50 mM 0.0934 mL 0.4668 mL 0.9336 mL 2.3340 mL
    60 mM 0.0778 mL 0.3890 mL 0.7780 mL 1.9450 mL
    80 mM 0.0584 mL 0.2918 mL 0.5835 mL 1.4588 mL
    100 mM 0.0467 mL 0.2334 mL 0.4668 mL 1.1670 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Amifostine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Amifostine20537-88-6WR2721WR 2721WR-2721MDM-2/p53HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHBroad-spectrumradioprotectorcytoprotectivethiolantiangiogenicHIF-α1p53DNA-damageInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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