Amifostine trihydrate (Synonyms: WR2721 trihydrate)

Name: Amifostine trihydrate
Brand: MedChemExpress
SKU: HY-B0639A
Rating: 5.0 (13 reviews)

Cat. No.: HY-B0639A Purity: 99.95%: Data Sheet
COA

SDS
Handling Instructions
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Amifostine trihydrate (WR2721 trihydrate) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine trihydrate selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine trihydrate is potent hypoxia-inducible factor-α1 (HIF-α1) and p53 inducer. Amifostine trihydrate protects cells from damage by scavenging oxygen-derived free radicals. Amifostine trihydrate reduces renal toxicity and has antiangiogenic action.

For research use only. We do not sell to patients.

CAS No. : 112901-68-5

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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Based on 13 publication(s) in Google Scholar

Other Forms of Amifostine trihydrate:

Amifostine In-stock

13 Publications Citing Use of MCE Amifostine trihydrate

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

Histological Imaging/Staining

RT-PCR

: Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Feb 13;6(2):e70092. [Abstract]; The dynamics of the numbers of CFUs in the bone marrow from indicated groups within a month post‐irradiation. The results showed that the reduction in colony-forming unit (CFU) counts in mice treated with Amifostine (150 mg/kg; i.p.; single dose) was relatively mild at 7 days post-irradiation.

: Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]; HE staining of testis among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

: Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]; Protein level alterations of Bclaf1, P-Rela, Rela, Sycp3, and β-actin were analyzed among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

: Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]; RT-qPCR analysis of Spliceosome-related gene expression levels across the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

: Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Bioact Mater.2024 May 21:39:191-205. [Abstract]; Cell viabilities of A31 cells under different doses of IR (0, 2, 4, 6, 8, and 10 Gy) with pretreatment of tFNAs, Amifostine (AMF, 54 μg/mL; 12 h), or nanosuit (250 nM; 12 h) (n = 5).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Amifostine (0.78125-100 μM, 24 h) trihydrate reduces tert-Butyl hydroperoxide (TBHP)-induced cell damage in a dose-dependent manner and significantly reduces H9c2 cells apoptosis at a concentration of 100 μM^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Amifostine (i.v., 400 mg/kg, 4 h) trihydrate has a protective effect against myocardial I/R injury in male C57BL/6 mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice with myocardial I/R injury^[5]
Dosage:	400 mg/kg
Administration:	Intravenous injection; 4 hours
Result:	Attenuated cardiomyocyte apoptosis and reduced the production of I/R-induced ROS. Significantly reduced the expression of cleaved caspase 3 and Bax while enhanced the expression of SOD1, SOD2 and Bcl2. Significantly increased SOD activity and reduced MDA levels.

Molecular Weight

268.27

Formula

C₅H₂₁N₂O₆PS

CAS No.

112901-68-5

Appearance

Solid

Color

White to off-white

SMILES

OP(SCCNCCCN)(O)=O.[H]O[H].[H]O[H].[H]O[H]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : 70 mg/mL (260.93 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7276 mL	18.6379 mL	37.2759 mL
5 mM	0.7455 mL	3.7276 mL	7.4552 mL
10 mM	0.3728 mL	1.8638 mL	3.7276 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (274 KB) SDS (392 KB)

COA (247 KB) HNMR (259 KB) CNMR (256 KB) RP-HPLC (205 KB) MS (72 KB)

Handling Instructions (2659 KB)

References

[1]. D Maurici, et al. Amifostine (WR2721) restores transcriptional activity of specific p53 mutant proteins in a yeast functional assay. Oncogene. 2001 Jun 14;20(27):3533-40. [Content Brief]

[2]. Efstathia Giannopoulou, et al. Amifostine inhibits angiogenesis in vivo. J Pharmacol Exp Ther. 2003 Feb;304(2):729-37. [Content Brief]

[3]. Michael I Koukourakis, et al. Amifostine induces anaerobic metabolism and hypoxia-inducible factor 1 alpha. Cancer Chemother Pharmacol. 2004 Jan;53(1):8-14. [Content Brief]

[4]. John R Kouvaris, et al. Amifostine: the first selective-target and broad-spectrum radioprotector. Oncologist. 2007 Jun;12(6):738-47. [Content Brief]

[5]. Shao-Ze Wu, et al. Amifostine Pretreatment Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Apoptosis and Oxidative Stress. Oxid Med Cell Longev. 2017;2017:4130824. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.7276 mL	18.6379 mL	37.2759 mL	93.1897 mL
	5 mM	0.7455 mL	3.7276 mL	7.4552 mL	18.6379 mL
	10 mM	0.3728 mL	1.8638 mL	3.7276 mL	9.3190 mL
	15 mM	0.2485 mL	1.2425 mL	2.4851 mL	6.2126 mL
	20 mM	0.1864 mL	0.9319 mL	1.8638 mL	4.6595 mL
	25 mM	0.1491 mL	0.7455 mL	1.4910 mL	3.7276 mL
	30 mM	0.1243 mL	0.6213 mL	1.2425 mL	3.1063 mL
	40 mM	0.0932 mL	0.4659 mL	0.9319 mL	2.3297 mL
	50 mM	0.0746 mL	0.3728 mL	0.7455 mL	1.8638 mL
	60 mM	0.0621 mL	0.3106 mL	0.6213 mL	1.5532 mL
	80 mM	0.0466 mL	0.2330 mL	0.4659 mL	1.1649 mL
	100 mM	0.0373 mL	0.1864 mL	0.3728 mL	0.9319 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Amifostine trihydrate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Amifostine trihydrate (Synonyms: WR2721 trihydrate)

Customer Review

Other Forms of Amifostine trihydrate:

Amifostine trihydrate Related Antibodies

13 Publications Citing Use of MCE Amifostine trihydrate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HIF/HIF Prolyl-Hydroxylase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Amifostine trihydrate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Amifostine trihydrate Related Classifications

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Customer Review

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