Amifostine trihydrate
Based on 16 publication(s) in Google Scholar
Amifostine trihydrate (WR2721 trihydrate) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine trihydrate selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine trihydrate is potent hypoxia-inducible factor-α1 (HIF-α1) and p53 inducer. Amifostine trihydrate protects cells from damage by scavenging oxygen-derived free radicals. Amifostine trihydrate reduces renal toxicity and has antiangiogenic action.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 112901-68-5
- Formula: C5H21N2O6PS
- Molecular Weight:268.27
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Amifostine trihydrate
More- Mol Cancer. 2024 Oct 19;23(1):234. [Abstract]
- Bioact Mater.2024 May 21:39:191-205. [Abstract]
- Nat Commun. 2025 Dec 8. [Abstract]
- Exp Mol Med. 2026 May;58(5):1642-1656. [Abstract]
- MedComm (2020). 2025 Feb 13;6(2):e70092. [Abstract]
- Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
- Int J Nanomedicine. 2024 Dec 6:19:13135-13148. [Abstract]
- ACS Appl Mater Interfaces. 2023 Mar 29;15(12):15203-15219. [Abstract]
- Int J Radiat Oncol Biol Phys. 2026 Apr 17:S0360-3016(26)00591-2. [Abstract]
- mLife. 2026 Apr 30;5(2):199-216. [Abstract]
- Int J Mol Sci. 2025 May 29;26(11):5238. [Abstract]
- Int Immunopharmacol. 2020 Nov;88:106998. [Abstract]
- Sci Rep. 2023 Jun 28;13(1):10485. [Abstract]
- Cancers (Basel). 2024 Jan 13;16(2):351. [Abstract]
- arXiv. 2025 Aug 28.
- University of Pardubice. 2023 Apr 27.
Customer Validation & Images
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In Vivo Efficacy Study
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Histological Imaging/Staining
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WB
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RT-PCR
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Cell Proliferation/Viability Assay
Biological Activity
Amifostine (0.78125-100 μM, 24 h) trihydrate reduces tert-Butyl hydroperoxide (TBHP)-induced cell damage in a dose-dependent manner and significantly reduces H9c2 cells apoptosis at a concentration of 100 μM[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6 mice with myocardial I/R injury[5]
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Dosage:400 mg/kg
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Administration:Intravenous injection; 4 hours
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Result:Attenuated cardiomyocyte apoptosis and reduced the production of I/R-induced ROS.
Significantly reduced the expression of cleaved caspase 3 and Bax while enhanced the expression of SOD1, SOD2 and Bcl2.
Significantly increased SOD activity and reduced MDA levels.
Chemical Information
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CAS No. 112901-68-5
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Appearance Solid
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Molecular Weight 268.27
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Formula C5H21N2O6PS
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Color White to off-white
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SMILES
OP(SCCNCCCN)(O)=O.[H]O[H].[H]O[H].[H]O[H]
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Synonyms
WR2721 trihydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (16)
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Journal Impact Factor
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Most Recent
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Mol Cancer
Anti irradiation nanoparticles shelter immune organ from radio-damage via preventing the IKK/IκB/NF-κB activation. [Abstract]2024 Oct 19;23(1):234. PMID: 39425231 -
Bioact Mater
A DNA tetrahedron-based nanosuit for efficient delivery of amifostine and multi-organ radioprotection. [Abstract]2024 May 21:39:191-205. PMID: 38808157
Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Bioact Mater.2024 May 21:39:191-205. [Abstract]
Cell viabilities of A31 cells under different doses of IR (0, 2, 4, 6, 8, and 10 Gy) with pretreatment of tFNAs, Amifostine (AMF, 54 μg/mL; 12 h), or nanosuit (250 nM; 12 h) (n = 5).
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Nat Commun
2025 Dec 8. PMID: 41361241 -
Exp Mol Med
Cannabidiol triggers fatty acids β-oxidation mediated by Stat2 to facilitate intestinal stem cells regeneration post radiation. [Abstract]2026 May;58(5):1642-1656. PMID: 42120478 -
MedComm (2020)
Cannabidiol restores hematopoietic stem cell stemness in mouse through Atf2-Lrp6 axis after acute irradiation. [Abstract]2025 Feb 13;6(2):e70092. PMID: 39949985
Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Feb 13;6(2):e70092. [Abstract]
The dynamics of the numbers of CFUs in the bone marrow from indicated groups within a month post‐irradiation. The results showed that the reduction in colony-forming unit (CFU) counts in mice treated with Amifostine (150 mg/kg; i.p.; single dose) was relatively mild at 7 days post-irradiation.
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Cell Commun Signal
Ionizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction. [Abstract]2025 Jan 31;23(1):58. PMID: 39891142
Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
HE staining of testis among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.
Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
Protein level alterations of Bclaf1, P-Rela, Rela, Sycp3, and β-actin were analyzed among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.
Amifostine trihydrate purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
RT-qPCR analysis of Spliceosome-related gene expression levels across the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.
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Int J Nanomedicine
Self-Assembled EGCG Nanoparticles with Enhanced Intracellular ROS Scavenging for Skin Radioprotection. [Abstract]2024 Dec 6:19:13135-13148. PMID: 39670199 -
ACS Appl Mater Interfaces
Amifostine-Loaded Nanocarrier Traverses the Blood-Brain Barrier and Prevents Radiation-Induced Brain Injury. [Abstract]2023 Mar 29;15(12):15203-15219. PMID: 36917732 -
Int J Radiat Oncol Biol Phys
Unraveling the Redox Mechanisms Underlying FLASH Radiotherapy: Critical Dose Thresholds and NRF2-Driven Sparing of Tissue. [Abstract]2026 Apr 17:S0360-3016(26)00591-2. PMID: 42002152 -
mLife
The synthetic estradiol analog E0703 enhances Akkermansia muciniphila growth for radiation-induced intestinal damage repair. [Abstract]2026 Apr 30;5(2):199-216. PMID: 42079447 -
Int J Mol Sci
New Cellular Interactions Due to the Radioprotective Effect of N-Acetylcysteine in a Model of Radiation-Induced Pancreatitis. [Abstract]2025 May 29;26(11):5238. PMID: 40508047 -
Int Immunopharmacol
Amifostine ameliorates induction of experimental autoimmune encephalomyelitis: Effect on reactive oxygen species/NLRP3 pathway. [Abstract]2020 Nov;88:106998. PMID: 33182064 -
Sci Rep
Amifostine attenuates bleomycin-induced pulmonary fibrosis in mice through inhibition of the PI3K/Akt/mTOR signaling pathway. [Abstract]2023 Jun 28;13(1):10485. PMID: 37380638 -
Cancers (Basel)
Mitochondria-Targeted Nitronyl Nitroxide Radical Nanoparticles for Protection against Radiation-Induced Damage with Antioxidant Effects. [Abstract]2024 Jan 13;16(2):351. PMID: 38254840 -
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Solvent & Solubility
H2O : 70 mg/mL (260.93 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. D Maurici, et al. Amifostine (WR2721) restores transcriptional activity of specific p53 mutant proteins in a yeast functional assay. Oncogene. 2001 Jun 14;20(27):3533-40. [Content Brief]
[2]. Efstathia Giannopoulou, et al. Amifostine inhibits angiogenesis in vivo. J Pharmacol Exp Ther. 2003 Feb;304(2):729-37. [Content Brief]
[3]. Michael I Koukourakis, et al. Amifostine induces anaerobic metabolism and hypoxia-inducible factor 1 alpha. Cancer Chemother Pharmacol. 2004 Jan;53(1):8-14. [Content Brief]
[4]. John R Kouvaris, et al. Amifostine: the first selective-target and broad-spectrum radioprotector. Oncologist. 2007 Jun;12(6):738-47. [Content Brief]
[5]. Shao-Ze Wu, et al. Amifostine Pretreatment Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Apoptosis and Oxidative Stress. Oxid Med Cell Longev. 2017;2017:4130824. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.7276 mL | 18.6379 mL | 37.2759 mL | 93.1897 mL |
| 5 mM | 0.7455 mL | 3.7276 mL | 7.4552 mL | 18.6379 mL | |
| 10 mM | 0.3728 mL | 1.8638 mL | 3.7276 mL | 9.3190 mL | |
| 15 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL | 6.2126 mL | |
| 20 mM | 0.1864 mL | 0.9319 mL | 1.8638 mL | 4.6595 mL | |
| 25 mM | 0.1491 mL | 0.7455 mL | 1.4910 mL | 3.7276 mL | |
| 30 mM | 0.1243 mL | 0.6213 mL | 1.2425 mL | 3.1063 mL | |
| 40 mM | 0.0932 mL | 0.4659 mL | 0.9319 mL | 2.3297 mL | |
| 50 mM | 0.0746 mL | 0.3728 mL | 0.7455 mL | 1.8638 mL | |
| 60 mM | 0.0621 mL | 0.3106 mL | 0.6213 mL | 1.5532 mL | |
| 80 mM | 0.0466 mL | 0.2330 mL | 0.4659 mL | 1.1649 mL | |
| 100 mM | 0.0373 mL | 0.1864 mL | 0.3728 mL | 0.9319 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Amifostine trihydrate Related Classifications
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