Amifostine (Synonyms: WR2721)

Name: Amifostine
Brand: MedChemExpress
SKU: HY-B0639
Rating: 5.0 (13 reviews)

Cat. No.: HY-B0639 Purity: 99.95%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Amifostine (WR2721) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine is potent hypoxia-inducible factor-α1 (HIF-α1) and p53 inducer. Amifostine protects cells from damage by scavenging oxygen-derived free radicals. Amifostine reduces renal toxicity and has antiangiogenic action.

For research use only. We do not sell to patients.

CAS No. : 20537-88-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Amifostine:

Amifostine trihydrate In-stock
Amifostine sodium Get quote

13 Publications Citing Use of MCE Amifostine

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

Histological Imaging/Staining

RT-PCR

: Amifostine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Feb 13;6(2):e70092. [Abstract]; The dynamics of the numbers of CFUs in the bone marrow from indicated groups within a month post‐irradiation. The results showed that the reduction in colony-forming unit (CFU) counts in mice treated with Amifostine (150 mg/kg; i.p.; single dose) was relatively mild at 7 days post-irradiation.

: Amifostine purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]; HE staining of testis among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

: Amifostine purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]; Protein level alterations of Bclaf1, P-Rela, Rela, Sycp3, and β-actin were analyzed among the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

: Amifostine purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]; RT-qPCR analysis of Spliceosome-related gene expression levels across the control group, IR group, Amifostine (50 mg/kg; i.p.) group, NF-κB activator 2 group, and CU-T12-9 group.

: Amifostine purchased from MedChemExpress. Usage Cited in: Bioact Mater.2024 May 21:39:191-205. [Abstract]; Cell viabilities of A31 cells under different doses of IR (0, 2, 4, 6, 8, and 10 Gy) with pretreatment of tFNAs, Amifostine (AMF, 54 μg/mL; 12 h), or nanosuit (250 nM; 12 h) (n = 5).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Amifostine (0.78125-100 μM, 24 h) reduces tert-Butyl hydroperoxide (TBHP)-induced cell damage in a dose-dependent manner and significantly reduces H9c2 cells apoptosis at a concentration of 100 μM^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Amifostine (i.v., 400 mg/kg, 4 h) has a protective effect against myocardial I/R injury in male C57BL/6 mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice with myocardial I/R injury^[5]
Dosage:	400 mg/kg
Administration:	Intravenous injection; 4 hours
Result:	Attenuated cardiomyocyte apoptosis and reduced the production of I/R-induced ROS. Significantly reduced the expression of cleaved caspase 3 and Bax while enhanced the expression of SOD1, SOD2 and Bcl2. Significantly increased SOD activity and reduced MDA levels.

Clinical Trial

Molecular Weight

214.22

Formula

C₅H₁₅N₂O₃PS

CAS No.

20537-88-6

Appearance

Solid

Color

White to off-white

SMILES

OP(SCCNCCCN)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (466.81 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6681 mL	23.3405 mL	46.6810 mL
5 mM	0.9336 mL	4.6681 mL	9.3362 mL
10 mM	0.4668 mL	2.3340 mL	4.6681 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (466.81 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (272 KB) SDS (392 KB)

COA (242 KB) HNMR (213 KB) CNMR (219 KB) RP-HPLC (167 KB) MS (85 KB)

Handling Instructions (2659 KB)

References

[1]. Kouvaris, J.R., V.E. Kouloulias, and L.J. Vlahos, Amifostine: the first selective-target and broad-spectrum radioprotector. Oncologist, 2007. 12(6): p. 738-47. [Content Brief]

[2]. D Maurici, et al. Amifostine (WR2721) restores transcriptional activity of specific p53 mutant proteins in a yeast functional assay. Oncogene. 2001 Jun 14;20(27):3533-40. [Content Brief]

[3]. Efstathia Giannopoulou, et al. Amifostine inhibits angiogenesis in vivo. J Pharmacol Exp Ther. 2003 Feb;304(2):729-37. [Content Brief]

[4]. Michael I Koukourakis, et al. Amifostine induces anaerobic metabolism and hypoxia-inducible factor 1 alpha. Cancer Chemother Pharmacol. 2004 Jan;53(1):8-14. [Content Brief]

[5]. Shao-Ze Wu, et al. Amifostine Pretreatment Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Apoptosis and Oxidative Stress. Oxid Med Cell Longev. 2017;2017:4130824. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.6681 mL	23.3405 mL	46.6810 mL	116.7025 mL
	5 mM	0.9336 mL	4.6681 mL	9.3362 mL	23.3405 mL
	10 mM	0.4668 mL	2.3340 mL	4.6681 mL	11.6702 mL
	15 mM	0.3112 mL	1.5560 mL	3.1121 mL	7.7802 mL
	20 mM	0.2334 mL	1.1670 mL	2.3340 mL	5.8351 mL
	25 mM	0.1867 mL	0.9336 mL	1.8672 mL	4.6681 mL
	30 mM	0.1556 mL	0.7780 mL	1.5560 mL	3.8901 mL
	40 mM	0.1167 mL	0.5835 mL	1.1670 mL	2.9176 mL
	50 mM	0.0934 mL	0.4668 mL	0.9336 mL	2.3340 mL
	60 mM	0.0778 mL	0.3890 mL	0.7780 mL	1.9450 mL
	80 mM	0.0584 mL	0.2918 mL	0.5835 mL	1.4588 mL
	100 mM	0.0467 mL	0.2334 mL	0.4668 mL	1.1670 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Amifostine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Amifostine (Synonyms: WR2721)

Customer Review

Other Forms of Amifostine:

Amifostine Related Antibodies

13 Publications Citing Use of MCE Amifostine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HIF/HIF Prolyl-Hydroxylase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Amifostine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Amifostine Related Classifications

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Customer Review

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SAFETY DATA SHEET (SDS)

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