1. Cell Cycle/DNA Damage Apoptosis Anti-infection Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  2. Topoisomerase Apoptosis Antibiotic Bacterial Mitochondrial Metabolism Reactive Oxygen Species
  3. Ciprofloxacin

Ciprofloxacin  (Synonyms: Bay-09867)

Cat. No.: HY-B0356 Purity: 99.86%
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Ciprofloxacin (Bay-09867) is a potent, orally active topoisomerase IV inhibitor. Ciprofloxacin induces mitochondrial DNA and nuclear DNA damage and lead to mitochondrial dysfunction, ROS production. Ciprofloxacin has anti-proliferative activity and induces apoptosis. Ciprofloxacin is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity.

For research use only. We do not sell to patients.

Ciprofloxacin Chemical Structure

Ciprofloxacin Chemical Structure

CAS No. : 85721-33-1

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Customer Review

Based on 22 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

Ciprofloxacin (Bay-09867) is a potent, orally active topoisomerase IV inhibitor. Ciprofloxacin induces mitochondrial DNA and nuclear DNA damage and lead to mitochondrial dysfunction, ROS production. Ciprofloxacin has anti-proliferative activity and induces apoptosis. Ciprofloxacin is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity[1][2][3][4].

IC50 & Target

Quinolone

 

In Vitro

Ciprofloxacin (Bay-09867) (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase[1].
? Ciprofloxacin (Bay-09867) shows potent activity against Y. pestis and B. anthracis with MIC90 of 0.03 μg/mL and 0.12 μg/mL, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis[1]

Cell Line: Tendon cells
Concentration: 5, 10, 20 and 50 μg/mL
Incubation Time: 24 hours
Result: Decreased the cellularity of tendon cells.

Apoptosis Analysis[1]

Cell Line: Tendon cells
Concentration: 50 μg/mL
Incubation Time: 24 hours
Result: Arrested cell cycle at the G2/M phase and inhibited cell division in tendon cells.

Western Blot Analysis[1]

Cell Line: Tendon cells
Concentration: 50 μg/mL
Incubation Time: 0, 6, 12, 17 and 24 hours
Result: Down-regulated the expression of CDK-1 and cyclin B protein and mRNA. Up-regulated the expression of PLK-1 protein.
In Vivo

Ciprofloxacin (Bay-09867) (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection against Y. pestis in murine model of pneumonic plague[3].
? Ciprofloxacin (Bay-09867) (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall[4].
? Ciprofloxacin (Bay-09867) (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice[3]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection; for 24 hours
Result: Reduced the lung bacterial load in murine model of pneumonic plague.
Animal Model: C57BL/6J mice[4]
Dosage: 100 mg/kg
Administration: Oral gavage; daily, for 4 weeks
Result: Had aortic destruction that was accompanied by decreased LOX expression and increased MMP expression and activity.
Animal Model: C57BL/6J mice[4]
Dosage: 100 mg/kg
Administration: Oral gavage; daily, for 4 weeks
Result: Caused mitochondrial DNA and nuclear DNA damage, leading to mitochondrial dysfunction and ROS production. Increased apoptosis and necroptosis in the aortic wall.
Clinical Trial
Molecular Weight

331.34

Formula

C17H18FN3O3

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CN(C2CC2)C3=C(C=C(F)C(N4CCNCC4)=C3)C1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

0.1 M HCL : 16.67 mg/mL (50.31 mM; ultrasonic and warming and adjust pH to 2 with HCl and heat to 60°C)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0180 mL 15.0902 mL 30.1805 mL
5 mM 0.6036 mL 3.0180 mL 6.0361 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
0.1 M HCL 1 mM 3.0180 mL 15.0902 mL 30.1805 mL 75.4512 mL
5 mM 0.6036 mL 3.0180 mL 6.0361 mL 15.0902 mL
10 mM 0.3018 mL 1.5090 mL 3.0180 mL 7.5451 mL
15 mM 0.2012 mL 1.0060 mL 2.0120 mL 5.0301 mL
20 mM 0.1509 mL 0.7545 mL 1.5090 mL 3.7726 mL
25 mM 0.1207 mL 0.6036 mL 1.2072 mL 3.0180 mL
30 mM 0.1006 mL 0.5030 mL 1.0060 mL 2.5150 mL
40 mM 0.0755 mL 0.3773 mL 0.7545 mL 1.8863 mL
50 mM 0.0604 mL 0.3018 mL 0.6036 mL 1.5090 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ciprofloxacin
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