1. Anti-infection
  2. Bacterial

Ciprofloxacin 

Cat. No.: HY-B0356 Purity: 98.74%
Handling Instructions

Ciprofloxacin is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity.

For research use only. We do not sell to patients.

Ciprofloxacin Chemical Structure

Ciprofloxacin Chemical Structure

CAS No. : 85721-33-1

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1 g USD 72 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 g USD 108 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
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Other Forms of Ciprofloxacin:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Ciprofloxacin is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity.

In Vitro

Ciprofloxacin is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity[1]. Ciprofloxacin (CIP) shows potent activity against Y. pestis with MIC90 of 0.03 μg/mL[2].

In Vivo

Ciprofloxacin (1 mg/L) induces glutathione-S-transferase (GST) activity, in contrast with inhibited GST and Catalase (CAT) of larvae exposed to enrofloxacin. Ciprofloxacin (≥10 μg/L) and enrofloxacin are ecotoxic for development, growth, detoxifying, and oxidative stress enzymes in anuran amphibian larvae[1]. In a murine model of pneumonic plague, Ciprofloxacin (30 mg/kg, i.p.) results in a drug exposure which is similar to the drug exposure observed in human following a 500 mg dose of oral Ciprofloxacin. Intraperitoneal Ciprofloxacin reduces the lung bacterial load compare to controls treated with intraperitoneal PBS[3].

Clinical Trial
References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 3.0180 mL 15.0902 mL 30.1805 mL
5 mM 0.6036 mL 3.0180 mL 6.0361 mL
10 mM 0.3018 mL 1.5090 mL 3.0180 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[2]

Antibiotics (including Ciprofloxacin) are serially diluted 2-fold in 50 μL of cation-adjusted Mueller-Hinton broth (CAMHB). The antibiotic (including Ciprofloxacin) ranges are 8 to 0.004 μg/mL or 64 to 0.03 μg/mL based on a final well volume of 100 μL after inoculation.

Bacterial inocula are prepared by suspending colonies into Mueller-Hinton broth (CAMHB) (containing Ciprofloxacin) from 18 to 24 h (B. anthracis) or 42 to 48 h (Y. pestis) on sheep blood agar (SBA) plates that are incubated at 35°C. Suspended cultures are diluted with CAMHB to a bacterial cell density of 105 CFU/mL adjusted based on the optical density at 600 nm. To each well of the 96-well plate, 50 μL of dilutions is added for a final inoculum of ~5×104 CFU/well. Plates are incubated at 35°C. MICs are determined visually at 18 to 24 h (B. anthracis) or 42 to 48 h (Y. pestis) and also by absorbance at 600 nm[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Female BALB/cAnNCrl (BALB/c) mice, 8 to 10 weeks old and 20 g (±4 g) are used in this assay. A single dose of Ciprofloxacin (30 mg/kg) is administered to mice (n=30) via the intraperitoneal (i.p.) route. The mice (n=3/time point/group) are culled at 1, 10, 20, or 30 min and 1, 1.5, 2, 4, 8, 12 h following Ciprofloxacin administration and 1, 15, or 30 min and 1, 2, 4, 6, 10, 18, or 24 h following DRCFI or CFI administration. Blood sampling points are chosen based upon the short half-life of Ciprofloxacin and longer half-life of CFI. Blood and lungs (whole organ) are collected post mortem for analysis. The lung doses following CFI or DRCFI administration are calculated using the concentration of Ciprofloxacin in the lung samples at 1 min post-administration[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

331.34

Formula

C₁₇H₁₈FN₃O₃

CAS No.

85721-33-1

SMILES

O=C(C1=CN(C2CC2)C3=C(C=C(F)C(N4CCNCC4)=C3)C1=O)O

Storage

4°C, protect from light

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: < 0.1 mg/mL; Acetic acid: ≥ 165 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Ciprofloxacin
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HY-B0356
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