2. Cell Cycle/DNA Damage Apoptosis Anti-infection Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. Topoisomerase Apoptosis Antibiotic Bacterial Mitochondrial Metabolism Reactive Oxygen Species (ROS)
  4. Ciprofloxacin hydrochloride monohydrate

Ciprofloxacin hydrochloride monohydrate  (Synonyms: Bay-09867 hydrochloride monohydrate)

Cat. No.: HY-B0356B Purity: 99.88%
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Ciprofloxacin (Bay-09867) hydrochloride monohydrate is a potent, orally active topoisomerase IV inhibitor. Ciprofloxacin hydrochloride monohydrate induces mitochondrial DNA and nuclear DNA damage and lead to mitochondrial dysfunction, ROS production. Ciprofloxacin hydrochloride monohydrate has anti-proliferative activity and induces apoptosis. Ciprofloxacin hydrochloride monohydrate is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity.

For research use only. We do not sell to patients.

CAS No. : 86393-32-0

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Customer Review

Based on 52 publication(s) in Google Scholar

Other Forms of Ciprofloxacin hydrochloride monohydrate:

Ciprofloxacin hydrochloride monohydrate Related Antibodies

Top Publications Citing Use of Products

52 Publications Citing Use of MCE Ciprofloxacin hydrochloride monohydrate

Bio/Physico-chemical Assay
Microbiological Assay
Others
2D/3D Cell Culture and Differentiation
Flow Cytometry

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 May 1.

    The number of surviving bacteria after 24 h of treatment with free Ciprofloxacin monohydrochloride or Ciprofloxacin monohydrochloride-loaded liposomes at 658 a 3 μg/mL of Ciprofloxacin monohydrochloride concentration.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 May 1.

    The CI values for 660 antibacterial efficacy of blank liposomes and Ciprofloxacin monohydrochloride.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: Water Res. 2024 Nov 29:271:122885.  [Abstract]

    Ciprofloxacin (CIP, 3 or 8 μg/L, 0-7 days) caused concentration-dependent damage in M. aeruginosa cells.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: Water Res. 2024 Nov 29:271:122885.  [Abstract]

    Ciprofloxacin (CIP, 3 or 8 μg/L, 3 or 7 days) increased the release and decreased the biosynthesis of MC-LR in M. aeruginosa cells.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: Water Res. 2024 Nov 29:271:122885.  [Abstract]

    Ciprofloxacin (CIP, 8 μg/L, 1 day) enriched gene ontology terms related to PSI in M. aeruginosa cells.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: Water Res. 2024 Nov 29:271:122885.  [Abstract]

    Ciprofloxacin (CIP, 3 or 8 μg/L, 21 days) induced continuous cell lysis, with maximum lytic rates of ∼70% compared to the initial cell density.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: Water Res. 2024 Nov 29:271:122885.  [Abstract]

    Ciprofloxacin (CIP, 3 or 8 μg/L, 1, 3, 5, or 7 days) induced cell death in M. aeruginosa.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: ACS Appl Polym Mater. 2023 Aug 29.

    Representative TEM images of blank micelles (BM) and Ciprofloxacin monohydrochloride-loaded micelles (CM) formulated in 1× PBS at pH 7.4.

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: ACS Appl Polym Mater. 2023 Aug 29.

    Cumulative ciprofloxacin release from Ciprofloxacin monohydrochloride-loaded micelles over 72 h with an inset of the release over the first 12 h at pH 4.4 (red) and pH 7.4 (gray).

    Ciprofloxacin hydrochloride monohydrate purchased from MedChemExpress. Usage Cited in: ACS Appl Polym Mater. 2023 Aug 29.

    Cumulative Ciprofloxacin monohydrochloride release at 12 and 72 h.

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    Description

    Ciprofloxacin (Bay-09867) hydrochloride monohydrate is a potent, orally active topoisomerase IV inhibitor. Ciprofloxacin hydrochloride monohydrate induces mitochondrial DNA and nuclear DNA damage and lead to mitochondrial dysfunction, ROS production. Ciprofloxacin hydrochloride monohydrate has anti-proliferative activity and induces apoptosis. Ciprofloxacin hydrochloride monohydrate is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity[1][2][3][4].

    IC50 & Target

    Quinolone

     

    In Vitro

    Ciprofloxacin (Bay-09867) hydrochloride monohydrate (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase[1].
    Ciprofloxacin (Bay-09867) hydrochloride monohydrate shows potent activity against Y. pestis and B. anthracis with MIC90 of 0.03 μg/mL and 0.12 μg/mL, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ciprofloxacin hydrochloride monohydrate Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Tendon cells
    Concentration: 5, 10, 20 and 50 μg/mL
    Incubation Time: 24 hours
    Result: Decreased the cellularity of tendon cells.

    Cell Cycle Analysis[1]

    Cell Line: Tendon cells
    Concentration: 50 μg/mL
    Incubation Time: 24 hours
    Result: Arrested cell cycle at the G2/M phase and inhibited cell division in tendon cells.

    Western Blot Analysis[1]

    Cell Line: Tendon cells
    Concentration: 50 μg/mL
    Incubation Time: 0, 6, 12, 17 and 24 hours
    Result: Down-regulated the expression of CDK-1 and cyclin B protein and mRNA. Up-regulated the expression of PLK-1 protein.
    In Vivo

    Ciprofloxacin (Bay-09867) hydrochloride monohydrate (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection against Y. pestis in murine model of pneumonic plague[3].
    Ciprofloxacin (Bay-09867) hydrochloride monohydrate (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall[4].
    Ciprofloxacin (Bay-09867) hydrochloride monohydrate (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c mice[3]
    Dosage: 30 mg/kg
    Administration: Intraperitoneal injection; for 24 hours
    Result: Reduced the lung bacterial load in murine model of pneumonic plague.
    Animal Model: C57BL/6J mice[4]
    Dosage: 100 mg/kg
    Administration: Oral gavage; daily, for 4 weeks
    Result: Had aortic destruction that was accompanied by decreased LOX expression and increased MMP expression and activity.
    Animal Model: C57BL/6J mice[4]
    Dosage: 100 mg/kg
    Administration: Oral gavage; daily, for 4 weeks
    Result: Caused mitochondrial DNA and nuclear DNA damage, leading to mitochondrial dysfunction and ROS production. Increased apoptosis and necroptosis in the aortic wall.
    Molecular Weight

    385.82

    Formula

    C17H21ClFN3O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [H]O[H].[H]Cl.O=C(C1=CN(C2CC2)C3=C(C=C(F)C(N4CCNCC4)=C3)C1=O)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 20 mg/mL (51.84 mM; Need ultrasonic)

    DMSO : 5 mg/mL (12.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5919 mL 12.9594 mL 25.9188 mL
    5 mM 0.5184 mL 2.5919 mL 5.1838 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.5 mg/mL (1.30 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.5 mg/mL (1.30 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.88%

    SDS (393 KB)

    COA (251 KB) RP-HPLC (219 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.5919 mL 12.9594 mL 25.9188 mL 64.7971 mL
    5 mM 0.5184 mL 2.5919 mL 5.1838 mL 12.9594 mL
    10 mM 0.2592 mL 1.2959 mL 2.5919 mL 6.4797 mL
    H2O 15 mM 0.1728 mL 0.8640 mL 1.7279 mL 4.3198 mL
    20 mM 0.1296 mL 0.6480 mL 1.2959 mL 3.2399 mL
    25 mM 0.1037 mL 0.5184 mL 1.0368 mL 2.5919 mL
    30 mM 0.0864 mL 0.4320 mL 0.8640 mL 2.1599 mL
    40 mM 0.0648 mL 0.3240 mL 0.6480 mL 1.6199 mL
    50 mM 0.0518 mL 0.2592 mL 0.5184 mL 1.2959 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Ciprofloxacin hydrochloride monohydrate Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ciprofloxacin hydrochloride86393-32-0Bay-09867 hydrochlorideTopoisomeraseApoptosisAntibioticBacterialMitochondrial MetabolismReactive Oxygen Species (ROS)mitochondrialDNADNA damageanti-proliferativeantibioticROSInhibitorinhibitorinhibit

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