D-AP5 (Synonyms: D-APV; D-2-Amino-5-phosphonovaleric acid)

Name: D-AP5
Brand: MedChemExpress
SKU: HY-100714A
Rating: 5.0 (29 reviews)

Cat. No.: HY-100714A Purity: 99.94%: Data Sheet
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D-AP5 (D-APV) is a selective and competitive NMDA receptor antagonist with a K_d of 1.4 μM. D-AP5 (D-APV) inhibits the glutamate binding site of NMDA receptors.

For research use only. We do not sell to patients.

CAS No. : 79055-68-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 29 publication(s) in Google Scholar

Other Forms of D-AP5:

DL-AP5 In-stock
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D-AP5 (Standard) Get quote

29 Publications Citing Use of MCE D-AP5

In Vivo Efficacy Study

: D-AP5 purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Jan 27;23(1):e3003011. [Abstract]; Averaged amplitude of eEPSCs in the RSC-ACC projections was measured with the perfusion of D-AP5 (50 μm), GYKI53655 (100 μm), and CNQX (20 μm) sequentially under the presence of picrotoxin (50 μm).

: D-AP5 purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Jan 27;23(1):e3003011. [Abstract]; Sample traces of eEPSCs in the RSC-ACC projections with the perfusion of D-AP5 (50 μm), GYKI53655 (100 μm), and CNQX (20 μm) sequentially under the presence of picrotoxin (50 μm) were recorded.

: D-AP5 purchased from MedChemExpress. Usage Cited in: Theranostics. 2023 May 11;13(9):2946-2961. [Abstract]; D-AP5 (1 µg/µl dissolved in 10% DMSO in 0.9% saline, 0.5 µl/side, via microinjection into the mPFC) was administered. Representative images showing c-Fos expression in the mPFC of different groups were obtained.

: D-AP5 purchased from MedChemExpress. Usage Cited in: Theranostics. 2023 May 11;13(9):2946-2961. [Abstract]; D-AP5 (1 µg/µl dissolved in 10% DMSO in 0.9% saline, 0.5 µl/side, via microinjection into the mPFC) activated HPC-mPFC and ameliorated the LPS-induced spatial memory deficit, but the effect was blocked by glutamate receptor inhibitors (D-AP5 and NBQX).

: D-AP5 purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2023 Jan;29(1):390-401. [Abstract]; Treatment with the AMDAR inhibitor (NBQX) and NMDAR inhibitor (D-AP5) effectively reduce the expression of NMDAR (NR1, NR2A/2B) and AMPAR (GluA1, GluA2) in PFC, respectively.

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Biological Activity
Protocol
Purity & Documentation
References
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Description

D-AP5 (D-APV) is a selective and competitive NMDA receptor antagonist with a K_d of 1.4 μM. D-AP5 (D-APV) inhibits the glutamate binding site of NMDA receptors^[1]^[2].

IC₅₀ & Target

NMDA Receptor

In Vivo

D-AP5 is a NMDA receptor antagonist. Chronic intraventricular infusion of D-AP5 causes a parallel dose-dependent impairment of spatial learning and long-term potentiation (LTP) in vivo. Intracerebral concentrations of D-AP5 fail to induce any measurable sensorimotor disturbance when spatial learning is prevented^[2].
D-AP5 infusion is associated with a progressive reduction in swim speed over trials. D-AP5 causes sensorimotor disturbances in the spatial task, but these gradually worsened as the animals fail to learn. Rats treated with D-AP5 show a delay-dependent deficit in spatial memory in the delayed matching-to-place protocol for the water maze^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

197.13

Formula

C₅H₁₂NO₅P

CAS No.

79055-68-8

Appearance

Solid

Color

White to off-white

SMILES

N[C@H](CCCP(O)(O)=O)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (507.28 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0728 mL	25.3640 mL	50.7279 mL
5 mM	1.0146 mL	5.0728 mL	10.1456 mL
10 mM	0.5073 mL	2.5364 mL	5.0728 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (507.28 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (273 KB) SDS (394 KB)

COA (245 KB) HNMR (133 KB) CNMR (133 KB) RP-HPLC (238 KB)

Handling Instructions (2659 KB)

References

[1]. R H Evans, et al. The effects of a series of omega-phosphonic alpha-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations. Br J Pharmacol. 1982 Jan;75(1):65-75. [Content Brief]

[2]. Davis S, et al. The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro. J Neurosci. 1992 Jan;12(1):21-34. [Content Brief]

[3]. Morris RG, et al. N-methyl-d-aspartate receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning. Eur J Neurosci. 2013 Mar;37(5):700-17. [Content Brief]

Animal Administration
^[1]

Male Lister hooded rats are used in this study. They are housed individually and have free access to food and water at all times except for 12 hr prior to surgery. Animals are implanted with 30 mM D-AP5 (N=6) and tested in the water maze^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. R H Evans, et al. The effects of a series of omega-phosphonic alpha-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations. Br J Pharmacol. 1982 Jan;75(1):65-75. [Content Brief]

[2]. Davis S, et al. The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro. J Neurosci. 1992 Jan;12(1):21-34. [Content Brief]

[3]. Morris RG, et al. N-methyl-d-aspartate receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning. Eur J Neurosci. 2013 Mar;37(5):700-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.0728 mL	25.3640 mL	50.7279 mL	126.8199 mL
	5 mM	1.0146 mL	5.0728 mL	10.1456 mL	25.3640 mL
	10 mM	0.5073 mL	2.5364 mL	5.0728 mL	12.6820 mL
	15 mM	0.3382 mL	1.6909 mL	3.3819 mL	8.4547 mL
	20 mM	0.2536 mL	1.2682 mL	2.5364 mL	6.3410 mL
	25 mM	0.2029 mL	1.0146 mL	2.0291 mL	5.0728 mL
	30 mM	0.1691 mL	0.8455 mL	1.6909 mL	4.2273 mL
	40 mM	0.1268 mL	0.6341 mL	1.2682 mL	3.1705 mL
	50 mM	0.1015 mL	0.5073 mL	1.0146 mL	2.5364 mL
	60 mM	0.0845 mL	0.4227 mL	0.8455 mL	2.1137 mL
	80 mM	0.0634 mL	0.3170 mL	0.6341 mL	1.5852 mL
	100 mM	0.0507 mL	0.2536 mL	0.5073 mL	1.2682 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

D-AP5 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-AP579055-68-8D-APV D-2-Amino-5-phosphonovaleric acidiGluRIonotropic glutamate receptorsInhibitorinhibitorinhibit

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D-AP5 (Synonyms: D-APV; D-2-Amino-5-phosphonovaleric acid)

Customer Review

Other Forms of D-AP5:

D-AP5 Related Antibodies

29 Publications Citing Use of MCE D-AP5

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All iGluR Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

D-AP5 Related Classifications

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Customer Review

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