2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. iGluR
  4. DL-AP5

DL-AP5  (Synonyms: 2-APV; DL-2-Amino-5-phosphonovaleric acid)

Cat. No.: HY-100714 Purity: 99.70%
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DL-AP5 (2-APV) is a competitive NMDA (N-methyl-D-aspartate) receptor antagonist. DL-AP5 shows significantly antinociceptive activity. DL-AP5 specifically blocks on channels in the rabbit retina.

For research use only. We do not sell to patients.

CAS No. : 76326-31-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of DL-AP5:

DL-AP5 Related Antibodies

Top Publications Citing Use of Products
In Vivo Efficacy Study
Bio/Physico-chemical Assay

    DL-AP5 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 July 07.

    DL-AP5 (50 μM) abolished the training effect so that trained and untrained trajectories overlapped in the CNS-3D organoids.

    DL-AP5 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 July 07.

    DL-AP5 (50 μM) suppressed activity-dependent neuronal recruitment and blocked stimulation-driven LTP in CNS-3D organoid networks, as the number of unique neurons remained low under NMDA receptor blockade.

    DL-AP5 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 July 07.

    DL-AP5 (200 μM). Heat map of GSVA scores across 15 GO terms associated with learning, memory, LTP, and synaptic plasticity.

    DL-AP5 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Oct 28:136767.  [Abstract]

    Effects of a single injection of DL-AP5 (5 μg; i.t.) on the tactile and pressure withdrawal thresholds and heat withdrawal latency within 2 h in KD-Setd2 and NC rats.

    DL-AP5 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Oct 28:136767.  [Abstract]

    The mean changes of baseline values and the DL-AP5 (50 μM) effect on the frequency and amplitude of mEPSCs in spinal dorsal horn neurons of Setd2fl/fl and Setd2-cKO mice.

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    AMPA Receptor NMDA Receptor Kainate Receptor

    • Biological Activity

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    Description

    DL-AP5 (2-APV) is a competitive NMDA (N-methyl-D-aspartate) receptor antagonist. DL-AP5 shows significantly antinociceptive activity. DL-AP5 specifically blocks on channels in the rabbit retina[1][2][3].

    IC50 & Target

    NMDA Receptor

     

    Cellular Effect
    Cell Line Type Value Description References
    Pyramidal neuron IC50
    0.31 μM
    Compound: L-AP5
    sensitization of CA1 pyramidal neurons by 2 to depolarizations by L-AP4,L-AP5 and L-AP6 after exposure to quisqualic acid
    sensitization of CA1 pyramidal neurons by 2 to depolarizations by L-AP4,L-AP5 and L-AP6 after exposure to quisqualic acid
    		[PMID: 7966155]
    Pyramidal neuron IC50
    2.2 μM
    Compound: L-AP5
    sensitization of CA1 pyramidal neurons by 2 to depolarizations by L-AP4, L-AP5 and L-AP6 after reversal with L-alpha-AA
    sensitization of CA1 pyramidal neurons by 2 to depolarizations by L-AP4, L-AP5 and L-AP6 after reversal with L-alpha-AA
    		[PMID: 7966155]
    Pyramidal neuron IC50
    2.3 μM
    Compound: L-AP5
    sensitization of CA1 pyramidal neurons by 2 to depolarizations by L-AP4, L-AP5 and L-AP6 before exposure to quisqualic acid
    sensitization of CA1 pyramidal neurons by 2 to depolarizations by L-AP4, L-AP5 and L-AP6 before exposure to quisqualic acid
    		[PMID: 7966155]
    In Vitro

    DL-AP5 (100 μM) partially prevents glutamate-induced increase in Arc/Arg3.1 protein levels[5].
    DL-AP5 decreases the NMDA-induced Arc/Arg3.1 upregulation[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DL-AP5 Related Antibodies
    In Vivo

    DL-AP5 (0-10 μg/rat, Intra-CA1) significantly decreases the effect of NMDA[3].
    DL-AP5 (0-10 nmol, Intracerebroventricular injection) causes a dose-dependent increase in food consumption[4].
    DL-AP5 (5 nmol, Intracerebroventricular injection) attenuates the decreased food consumption induced by the intracerebroventricular injection of ghrelin[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rats (180-230 g)[3]
    Dosage: 1, 3.2 and 10 μg/rat
    Administration: Injected into the intra-dorsal hippocampal (intra-CA1) immediately after shock administration, once
    Result: Significantly decreased the effect of NMDA (10-2 μg/rat, intra-CA1) with significant interaction.
    Animal Model: Broilers cockerels (3-h fooddeprived (FD3), n=8 for each group)[4]
    Dosage: 0, 2.5, 5, and 10 nmol; in a volume of 10 μL
    Administration: Intracerebroventricular injection
    Result: Caused a dose-dependent increase in food consumption which was significant for 5 and 10 nmol doses.
    Animal Model: Broilers cockerels (3-h fooddeprived (FD3), n=8 for each group)[4]
    Dosage: 5 nmol
    Administration: Intracerebroventricular injection, followed by ghrelin (0.6 nmol)
    Result: Attenuated the decreased food consumption induced by the intracerebroventricular injection of ghrelin.
    Molecular Weight

    197.13

    Formula

    C5H12NO5P
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(CCCP(O)(O)=O)C(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 33.33 mg/mL (169.08 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.0728 mL 25.3640 mL 50.7279 mL
    5 mM 1.0146 mL 5.0728 mL 10.1456 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (253.64 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.70%

    SDS (393 KB)

    COA (244 KB) HNMR (247 KB) CNMR (311 KB) RP-HPLC (237 KB) MS (81 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.0728 mL 25.3640 mL 50.7279 mL 126.8199 mL
    5 mM 1.0146 mL 5.0728 mL 10.1456 mL 25.3640 mL
    10 mM 0.5073 mL 2.5364 mL 5.0728 mL 12.6820 mL
    15 mM 0.3382 mL 1.6909 mL 3.3819 mL 8.4547 mL
    20 mM 0.2536 mL 1.2682 mL 2.5364 mL 6.3410 mL
    25 mM 0.2029 mL 1.0146 mL 2.0291 mL 5.0728 mL
    30 mM 0.1691 mL 0.8455 mL 1.6909 mL 4.2273 mL
    40 mM 0.1268 mL 0.6341 mL 1.2682 mL 3.1705 mL
    50 mM 0.1015 mL 0.5073 mL 1.0146 mL 2.5364 mL
    60 mM 0.0845 mL 0.4227 mL 0.8455 mL 2.1137 mL
    80 mM 0.0634 mL 0.3170 mL 0.6341 mL 1.5852 mL
    100 mM 0.0507 mL 0.2536 mL 0.5073 mL 1.2682 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    DL-AP5 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DL-AP576326-31-32-APV DL-2-Amino-5-phosphonovaleric acidiGluRIonotropic glutamate receptorsAntinociceptive activityTonic painGanglion cellsRetinaArc/Arg3.1Glutamate receptorInhibitorinhibitorinhibit

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