1. Membrane Transporter/Ion Channel
    Neuronal Signaling
    Metabolic Enzyme/Protease
  2. iGluR
    Endogenous Metabolite
  3. NMDA

NMDA (Synonyms: N-Methyl-D-aspartic acid)

Cat. No.: HY-17551 Purity: >98.0%
Handling Instructions

NMDA is a specific agonist for NMDA receptor mimicking the action of glutamate, the neurotransmitter which normally acts at that receptor.

For research use only. We do not sell to patients.

NMDA Chemical Structure

NMDA Chemical Structure

CAS No. : 6384-92-5

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10 mM * 1 mL in DMSO USD 66 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

    NMDA purchased from MCE. Usage Cited in: Neuroreport. 2017 May 24;28(8):444-450.

    Western blot results further confirm the decrease in GDF10 expression induced by NMDA.

    NMDA purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):827-834.

    Representative western blots for SDHA and COX IV in the different groups of RGC-5 cells after 24 h (GAPDH is used as the reference protein).
    • Biological Activity

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    Description

    NMDA is a specific agonist for NMDA receptor mimicking the action of glutamate, the neurotransmitter which normally acts at that receptor.

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    NMDA exerts a significant augmentation of the adrenal binding independently of the incubation temperature in a concentration-dependent manner[2].

    In Vivo

    NMDA (0.2 nM) shows significant effects on MF, IF, IL, and EL, respectively, decreasing the mount and intromission frequencies, and shortening the intromission and ejaculation latencies. NMDA and AP-5 significantly, respectively, facilitates and inhibits the ejaculatory behavior during the copulation testing 30 min. Bilateral microinjection of NMDA into PVN significantly increases the baseline LSNA, the peaking increment of LSNA occurred within 5 min from the time of NMDA microinjected into PVN[1].

    Molecular Weight

    147.13

    Formula

    C₅H₉NO₄

    CAS No.

    6384-92-5

    SMILES

    O=C(O)C[[email protected]](C(O)=O)NC

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (339.84 mM; Need ultrasonic)

    DMSO : 10 mg/mL (67.97 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.7967 mL 33.9836 mL 67.9671 mL
    5 mM 1.3593 mL 6.7967 mL 13.5934 mL
    10 mM 0.6797 mL 3.3984 mL 6.7967 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 1 mg/mL (6.80 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 1 mg/mL (6.80 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 1 mg/mL (6.80 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Kinase Assay
    [2]

    Adrenal membranous homogenate suspensions are incubated with 10 nM [3H]Glu in 500/zl 50 mM Tris-acetate buffer (pH 7.4) at 2°C or 30°C in the presence and absence of various compounds. Incubation is terminated by the addition of 3 mL ice-cold buffer and subsequent filtration through a Whatman GF/B glass filter under a constant vacuum of 15 mm Hg. After washing the filter 4 times with 3 mL icecold buffer, the radioactivity trapped on the filter is measured by a liquid scintillation spectrometer using 5 mL modified Triton-toluene scintillant at a counting efficiency of 40-42%. The radioactivity found in the presence of 1 mM non-radioactive Glu is subtracted from each experimental value to obtain the specific binding of [3H]GIu in accordance with the y-aminobutyric acid (GABA) receptor binding assay system. The kinetic parameters of [3H]GIu binding, Kd and Bma x, are calculated by Scatchard analysis of the specific binding using a personal computer with a programme for non-linear regression analysis developed in our own laboratory.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    Thirty male rats are paired with different receptive females for a total of three times (once every 3 days) a week prior to the experiment, only the males that ejaculated at least three times during this period are included. After selecting the male rats with normal ejaculatory ability. Saline (100 nL), NMDA (0.20 nmol in 100 nL saline), and AP-5 (10.0 nmol in 100 nL saline) are adminitration into the bilateral PVN of each male rat in random order. After 5 min, the behavioral testing is performed and recorded as described above. Copulatory behaviors occur once a week and the entire experiment lasted 4 weeks.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    NMDAN-Methyl-D-aspartic acidiGluREndogenous MetaboliteIonotropic glutamate receptorsInhibitorinhibitorinhibit

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