Gambogic Acid (Synonyms: Beta-Guttiferrin)

Name: Gambogic Acid
Brand: MedChemExpress
SKU: HY-N0087
Rating: 5.0 (6 reviews)

Cat. No.: HY-N0087 Pureza: 98.34%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

Gambogic Acid (Beta-Guttiferrin) is derived from the gamboges resin of the tree Garcinia hanburyi. Gambogic Acid (Beta-Guttiferrin) inhibits Bcl-X_L, Bcl-2, Bcl-W, Bcl-B, Bfl-1 and Mcl-1 with IC₅₀s of 1.47 μM, 1.21 μM, 2.02 μM, 0.66 μM, 1.06 μM and 0.79 μM.

Para uso exclusivo en investigación. No vendemos a pacientes.

Gambogic Acid Estructura química

No. CAS : 2752-65-0

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

This product is a controlled substance and not for sale in your territory.

Revisión del cliente

Based on 6 publication(s) in Google Scholar

Other Forms of Gambogic Acid:

Gambogic Acid (Standard) Obtener un presupuesto

6 Publications Citing Use of MCE Gambogic Acid

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Bcl-2 Family:

Ver todas las isoformas

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

Actividad biológica
Protocolo
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

IC₅₀ & Target^[1]

Bcl-B

0.66 μM (IC₅₀)

Mcl-1

0.79 μM (IC₅₀)

Bfl-1

1.06 μM (IC₅₀)

Bcl-2

1.21 μM (IC₅₀)

Bcl-xL

1.47 μM (IC₅₀)

Bcl-W

2.02 μM (IC₅₀)

Autophagy

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549	IC₅₀	1.1 3 Compound: GA	Antiproliferative activity against human A549 cells after 24 hrs by MTT assay Antiproliferative activity against human A549 cells after 24 hrs by MTT assay	[PMID: 22472167]
A549	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549	IC₅₀	0.5 6 Compound: GA	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 22595179]
A549	IC₅₀	1.2 3 Compound: 1; GA	Antiproliferative activity against human A549 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human A549 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
A549	IC₅₀	0.5 6 Compound: GA	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 22595179]
A549	IC₅₀	1.1 3 Compound: 1, GA	Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
A549	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549	IC₅₀	1.1 3 Compound: GA	Cytotoxicity against human A549 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
A549	IC₅₀	1.47 3 Compound: GA	Antiproliferative activity against human A549 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
A549	IC₅₀	1.1 3 Compound: GA	Antiproliferative activity against human A549 cells after 24 hrs by MTT assay Antiproliferative activity against human A549 cells after 24 hrs by MTT assay	[PMID: 22472167]
A549	IC₅₀	1.1 3 Compound: 1, GA	Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
A549	IC₅₀	1.1 3 Compound: GA	Cytotoxicity against human A549 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
A549	IC₅₀	1.1 3 Compound: GA	Antiproliferative activity against human A549 cells after 24 hrs by MTT assay Antiproliferative activity against human A549 cells after 24 hrs by MTT assay	[PMID: 22472167]
A549	IC₅₀	3.65 3 Compound: GA	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 20605273]
A549	IC₅₀	5.81 3 Compound: gambogic acid, GA	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 18996626]
A549	IC₅₀	1.2 3 Compound: 1; GA	Antiproliferative activity against human A549 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human A549 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
A549	IC₅₀	1.2 3 Compound: 1; GA	Antiproliferative activity against human A549 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human A549 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
A549	IC₅₀	1.47 3 Compound: GA	Antiproliferative activity against human A549 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
A549	IC₅₀	1.47 3 Compound: GA	Antiproliferative activity against human A549 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
A549	IC₅₀	1.1 3 Compound: 1, GA	Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
A549	IC₅₀	1.1 3 Compound: GA	Cytotoxicity against human A549 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
A549	IC₅₀	3.4 3 Compound: 4	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 to 48 hrs by Presto Blue assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 to 48 hrs by Presto Blue assay	[PMID: 27216998]
A549	IC₅₀	3.4 3 Compound: 4	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 to 48 hrs by Presto Blue assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 to 48 hrs by Presto Blue assay	[PMID: 27216998]
A549	IC₅₀	3.65 3 Compound: GA	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 20605273]
A549	IC₅₀	3.4 3 Compound: 4	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 to 48 hrs by Presto Blue assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 to 48 hrs by Presto Blue assay	[PMID: 27216998]
A549	IC₅₀	3.65 3 Compound: GA	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 20605273]
A549	IC₅₀	5.81 3 Compound: gambogic acid, GA	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 18996626]
A549	IC₅₀	5.81 3 Compound: gambogic acid, GA	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 18996626]
A549	IC₅₀	0.5 6 Compound: GA	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 22595179]
A549/CDDP	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549/CDDP cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549/CDDP cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549/CDDP	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549/CDDP cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549/CDDP cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549/TR	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549/CDDP	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549/CDDP cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549/CDDP cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549/TR	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
A549/TR	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
Bel-7402	IC₅₀	2.08 3 Compound: GA	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 20605273]
Bel-7402	IC₅₀	0.75 3 Compound: 1, GA	Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 22153338]
BGC-823	IC₅₀	2.35 3 Compound: 1, GA	Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay	[PMID: 21334116]
Bel-7402	IC₅₀	3.31 3 Compound: gambogic acid, GA	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 18996626]
Bel-7402	IC₅₀	2.08 3 Compound: GA	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 20605273]
BGC-823	IC₅₀	2.83 3 Compound: GA	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 20605273]
Bel-7402	IC₅₀	0.75 3 Compound: 1, GA	Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 22153338]
Bel-7402	IC₅₀	3.31 3 Compound: gambogic acid, GA	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 18996626]
BGC-823	IC₅₀	4.51 3 Compound: gambogic acid, GA	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 18996626]
BEL-7404 tumor cell line	IC₅₀	4.7 3 Compound: 1, GA	Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay	[PMID: 22153338]
BEL-7404 tumor cell line	IC₅₀	4.7 3 Compound: 1, GA	Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay	[PMID: 22153338]
BJ	EC₅₀	0.21 3 Compound: Gambogic acid	Cytotoxicity against human BJ cells Cytotoxicity against human BJ cells	[PMID: 25316317]
BGC-823	IC₅₀	2.35 3 Compound: 1, GA	Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay	[PMID: 21334116]
BGC-823	IC₅₀	2.35 3 Compound: 1, GA	Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay	[PMID: 21334116]
BGC-823	IC₅₀	2.83 3 Compound: GA	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 20605273]
Bel-7402	IC₅₀	0.75 3 Compound: 1, GA	Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 22153338]
BGC-823	IC₅₀	2.83 3 Compound: GA	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 20605273]
Bel-7402	IC₅₀	2.08 3 Compound: GA	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 20605273]
BGC-823	IC₅₀	4.51 3 Compound: gambogic acid, GA	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 18996626]
BGC-823	IC₅₀	4.51 3 Compound: gambogic acid, GA	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 18996626]
BJ	EC₅₀	0.21 3 Compound: Gambogic acid	Cytotoxicity against human BJ cells Cytotoxicity against human BJ cells	[PMID: 25316317]
Bel-7402	IC₅₀	3.31 3 Compound: gambogic acid, GA	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 18996626]
Breast carcinoma cell	IC₅₀	0.42 3 Compound: GBA; 1	Cytotoxicity against patient derived inflammatory breast cancer cell spheroids assessed as increase in spheroid dissolution incubated for 24 hrs by propidium iodide/Acridine orange staining based dual-fluorescence viability assay Cytotoxicity against patient derived inflammatory breast cancer cell spheroids assessed as increase in spheroid dissolution incubated for 24 hrs by propidium iodide/Acridine orange staining based dual-fluorescence viability assay	[PMID: 30831408]
Breast carcinoma cell	IC₅₀	0.42 3 Compound: GBA; 1	Cytotoxicity against patient derived inflammatory breast cancer cell spheroids assessed as increase in spheroid dissolution incubated for 24 hrs by propidium iodide/Acridine orange staining based dual-fluorescence viability assay Cytotoxicity against patient derived inflammatory breast cancer cell spheroids assessed as increase in spheroid dissolution incubated for 24 hrs by propidium iodide/Acridine orange staining based dual-fluorescence viability assay	[PMID: 30831408]
Breast carcinoma cell	IC₅₀	0.42 3 Compound: GBA; 1	Cytotoxicity against patient derived inflammatory breast cancer cell spheroids assessed as increase in spheroid dissolution incubated for 24 hrs by propidium iodide/Acridine orange staining based dual-fluorescence viability assay Cytotoxicity against patient derived inflammatory breast cancer cell spheroids assessed as increase in spheroid dissolution incubated for 24 hrs by propidium iodide/Acridine orange staining based dual-fluorescence viability assay	[PMID: 30831408]
DU-145	IC₅₀	0.3 6 Compound: GA	Cytotoxicity against human DU145 cells by MTT assay Cytotoxicity against human DU145 cells by MTT assay	[PMID: 22595179]
DU-145	IC₅₀	0.3 6 Compound: GA	Cytotoxicity against human DU145 cells by MTT assay Cytotoxicity against human DU145 cells by MTT assay	[PMID: 22595179]
HCT-116	GI₅₀	0.1 3 Compound: 1, GA	Growth inhibition of human HCT116 cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human HCT116 cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
HCT-116	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human HCT116 cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human HCT116 cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
HCT-116	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
HCT-116	GI₅₀	0.1 3 Compound: 1, GA	Growth inhibition of human HCT116 cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human HCT116 cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
HCT-116	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human HCT116 cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human HCT116 cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
DU-145	IC₅₀	0.3 6 Compound: GA	Cytotoxicity against human DU145 cells by MTT assay Cytotoxicity against human DU145 cells by MTT assay	[PMID: 22595179]
HCT-116	IC₅₀	3.11 3 Compound: GA	Antiproliferative activity against human HCT116 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
HCT-116	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
HCT-116	IC₅₀	3.11 3 Compound: GA	Antiproliferative activity against human HCT116 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
HEK293	IC₅₀	1.72 3 Compound: 4	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 24 to 48 hrs by trypan blue dye based fluorescence assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 24 to 48 hrs by trypan blue dye based fluorescence assay	[PMID: 27216998]
HEK293	EC₅₀	0.21 3 Compound: Gambogic acid	Cytotoxicity against HEK293 cells Cytotoxicity against HEK293 cells	[PMID: 25316317]
HeLa	IC₅₀	0.7 3 Compound: 6a, R-gambogic acid	Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay	[PMID: 19072548]
HCT-116	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human HCT116 cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human HCT116 cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
HeLa	GI₅₀	1.5 3 Compound: 1	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
HEK293	IC₅₀	1.72 3 Compound: 4	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 24 to 48 hrs by trypan blue dye based fluorescence assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 24 to 48 hrs by trypan blue dye based fluorescence assay	[PMID: 27216998]
HeLa	GI₅₀	1.5 3 Compound: 1	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
HCT-116	GI₅₀	0.1 3 Compound: 1, GA	Growth inhibition of human HCT116 cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human HCT116 cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
HepG2	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
HeLa	IC₅₀	0.7 3 Compound: 6a, R-gambogic acid	Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay	[PMID: 19072548]
HCT-116	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
HepG2	IC₅₀	0.89 3 Compound: GA	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
HCT-116	IC₅₀	3.11 3 Compound: GA	Antiproliferative activity against human HCT116 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
HepG2	EC₅₀	<2 3 Compound: Gambogic acid	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 25316317]
HepG2	IC₅₀	0.89 3 Compound: 1, GA	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
HEK293	EC₅₀	0.21 3 Compound: Gambogic acid	Cytotoxicity against HEK293 cells Cytotoxicity against HEK293 cells	[PMID: 25316317]
HepG2	IC₅₀	1.17 3 Compound: 1, GA	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 22153338]
HepG2	IC₅₀	2.4 3 Compound: 1, GA	Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 21334116]
HEK293	IC₅₀	1.72 3 Compound: 4	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 24 to 48 hrs by trypan blue dye based fluorescence assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 24 to 48 hrs by trypan blue dye based fluorescence assay	[PMID: 27216998]
HepG2	IC₅₀	2.19 3 Compound: 1; GA	Antiproliferative activity against human HepG2 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human HepG2 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
HepG2	IC₅₀	1.55 3 Compound: Gambogic acid	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay	[PMID: 30594028]
HL-60	GI₅₀	0.3 3 Compound: Gambogic acid	Growth inhibition of human HL60 cells measured after 3 days by trypan blue assay Growth inhibition of human HL60 cells measured after 3 days by trypan blue assay	[PMID: 29565129]
HepG2	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
HepG2	IC₅₀	2.19 3 Compound: 1; GA	Antiproliferative activity against human HepG2 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human HepG2 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
HL-60	IC₅₀	0.17 3 Compound: 54	Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 3 days by trypan blue dye based hemocytometer counting method Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 3 days by trypan blue dye based hemocytometer counting method	[PMID: 30830783]
HepG2	IC₅₀	2.36 3 Compound: GA	Antiproliferative activity against human HepG2 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
HepG2	IC₅₀	2.36 3 Compound: GA	Antiproliferative activity against human HepG2 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
HT-29	ED₅₀	0.48 3 Compound: 1	Anticancer activity aganist human HT-29 cells Anticancer activity aganist human HT-29 cells	[PMID: 21067206]
HepG2	IC₅₀	2.4 3 Compound: GA	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 20338759]
HepG2	IC₅₀	2.4 3 Compound: 1, GA	Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 21334116]
HT-29	IC₅₀	2.37 3 Compound: 1; GA	Antiproliferative activity against human HT-29 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human HT-29 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
HepG2	IC₅₀	1.17 3 Compound: 1, GA	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 22153338]
HepG2	IC₅₀	2.4 3 Compound: GA	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 20338759]
HT-29	IC₅₀	3.52 3 Compound: GA	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 20605273]
HepG2	IC₅₀	0.89 3 Compound: 1, GA	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
HL-60	IC₅₀	0.17 3 Compound: 54	Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 3 days by trypan blue dye based hemocytometer counting method Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 3 days by trypan blue dye based hemocytometer counting method	[PMID: 30830783]
HT-29	IC₅₀	5.61 3 Compound: gambogic acid, GA	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 18996626]
HL-60	GI₅₀	0.3 3 Compound: Gambogic acid	Growth inhibition of human HL60 cells measured after 3 days by trypan blue assay Growth inhibition of human HL60 cells measured after 3 days by trypan blue assay	[PMID: 29565129]
HepG2	IC₅₀	0.89 3 Compound: GA	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
HepG2	IC₅₀	1.55 3 Compound: Gambogic acid	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay	[PMID: 30594028]
HeLa	GI₅₀	1.5 3 Compound: 1	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
HT-29	IC₅₀	2.37 3 Compound: 1; GA	Antiproliferative activity against human HT-29 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human HT-29 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
HeLa	IC₅₀	0.7 3 Compound: 6a, R-gambogic acid	Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay	[PMID: 19072548]
HL-60	GI₅₀	0.3 3 Compound: Gambogic acid	Growth inhibition of human HL60 cells measured after 3 days by trypan blue assay Growth inhibition of human HL60 cells measured after 3 days by trypan blue assay	[PMID: 29565129]
HT-29	IC₅₀	3.52 3 Compound: GA	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 20605273]
HL-60	IC₅₀	0.17 3 Compound: 54	Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 3 days by trypan blue dye based hemocytometer counting method Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 3 days by trypan blue dye based hemocytometer counting method	[PMID: 30830783]
HT-29	IC₅₀	5.61 3 Compound: gambogic acid, GA	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 18996626]
HT-29	ED₅₀	0.48 3 Compound: 1	Anticancer activity aganist human HT-29 cells Anticancer activity aganist human HT-29 cells	[PMID: 21067206]
HepG2	EC₅₀	< 2 3 Compound: Gambogic acid	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 25316317]
K562	GI₅₀	0.6 3 Compound: Gambogic acid	Growth inhibition of human K562 cells measured after 3 days by trypan blue assay Growth inhibition of human K562 cells measured after 3 days by trypan blue assay	[PMID: 29565129]
HepG2	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
HT-29	IC₅₀	2.37 3 Compound: 1; GA	Antiproliferative activity against human HT-29 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human HT-29 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
K562	IC₅₀	0.62 3 Compound: Gambogic acid	Antiproliferative activity against imatinib resistant human K562 cells assessed as cell growth inhibition measured after 48 to 72 hrs by MTS assay Antiproliferative activity against imatinib resistant human K562 cells assessed as cell growth inhibition measured after 48 to 72 hrs by MTS assay	[PMID: 35551036]
HT-29	IC₅₀	3.52 3 Compound: GA	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 20605273]
HepG2	IC₅₀	0.89 3 Compound: 1, GA	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
KB	IC₅₀	0.3 6 Compound: GA	Cytotoxicity against human KB cells by MTT assay Cytotoxicity against human KB cells by MTT assay	[PMID: 22595179]
HepG2	IC₅₀	0.89 3 Compound: GA	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
HT-29	IC₅₀	5.61 3 Compound: gambogic acid, GA	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 18996626]
MCF7	IC₅₀	0.5 3 Compound: GA	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 20338759]
HepG2	IC₅₀	1.17 3 Compound: 1, GA	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 22153338]
K562	GI₅₀	0.6 3 Compound: Gambogic acid	Growth inhibition of human K562 cells measured after 3 days by trypan blue assay Growth inhibition of human K562 cells measured after 3 days by trypan blue assay	[PMID: 29565129]
MCF7	IC₅₀	1.25 3 Compound: 1; GA	Antiproliferative activity against human MCF7 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
HepG2	IC₅₀	1.55 3 Compound: Gambogic acid	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay	[PMID: 30594028]
K562	IC₅₀	0.62 3 Compound: Gambogic acid	Antiproliferative activity against imatinib resistant human K562 cells assessed as cell growth inhibition measured after 48 to 72 hrs by MTS assay Antiproliferative activity against imatinib resistant human K562 cells assessed as cell growth inhibition measured after 48 to 72 hrs by MTS assay	[PMID: 35551036]
MCF7	GI₅₀	2 3 Compound: 1	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
HepG2	IC₅₀	2.19 3 Compound: 1; GA	Antiproliferative activity against human HepG2 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human HepG2 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
KB	IC₅₀	0.3 6 Compound: GA	Cytotoxicity against human KB cells by MTT assay Cytotoxicity against human KB cells by MTT assay	[PMID: 22595179]
MCF7	IC₅₀	2.19 3 Compound: 1, GA	Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 21334116]
HepG2	IC₅₀	2.36 3 Compound: GA	Antiproliferative activity against human HepG2 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
MCF7	GI₅₀	2 3 Compound: 1	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
MDA-MB-231	IC₅₀	1.33 3 Compound: GA	Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
MCF7	IC₅₀	2.19 3 Compound: 1, GA	Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 21334116]
HepG2	IC₅₀	2.4 3 Compound: 1, GA	Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 21334116]
MKN-45	IC₅₀	4.27 3 Compound: GA	Antiproliferative activity against human MKN45 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MKN45 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
HepG2	IC₅₀	2.4 3 Compound: GA	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 20338759]
PC-3	IC₅₀	1.19 3 Compound: 1; GA	Antiproliferative activity against human PC3 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human PC3 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
MCF7	IC₅₀	1.25 3 Compound: 1; GA	Antiproliferative activity against human MCF7 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
SGC-7901	IC₅₀	1.47 3 Compound: GA	Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay	[PMID: 20338759]
MCF7	IC₅₀	0.5 3 Compound: GA	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 20338759]
K562	GI₅₀	0.6 3 Compound: Gambogic acid	Growth inhibition of human K562 cells measured after 3 days by trypan blue assay Growth inhibition of human K562 cells measured after 3 days by trypan blue assay	[PMID: 29565129]
SK-BR-3	GI₅₀	0.8 3 Compound: 1	Antiproliferative activity against human SKBR3 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human SKBR3 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
MDA-MB-231	IC₅₀	1.33 3 Compound: GA	Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
K562	IC₅₀	0.62 3 Compound: Gambogic acid	Antiproliferative activity against imatinib resistant human K562 cells assessed as cell growth inhibition measured after 48 to 72 hrs by MTS assay Antiproliferative activity against imatinib resistant human K562 cells assessed as cell growth inhibition measured after 48 to 72 hrs by MTS assay	[PMID: 35551036]
MKN-45	IC₅₀	4.27 3 Compound: GA	Antiproliferative activity against human MKN45 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MKN45 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
SK-BR-3	IC₅₀	0.89 3 Compound: 1; GA	Antiproliferative activity against human SK-BR-3 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human SK-BR-3 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
KB	IC₅₀	0.3 6 Compound: GA	Cytotoxicity against human KB cells by MTT assay Cytotoxicity against human KB cells by MTT assay	[PMID: 22595179]
PC-3	IC₅₀	1.19 3 Compound: 1; GA	Antiproliferative activity against human PC3 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human PC3 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
SK-OV-3	IC₅₀	3.06 3 Compound: gambogic acid, GA	Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay	[PMID: 18996626]
MCF7	GI₅₀	2 3 Compound: 1	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
Raji	EC₅₀	0.24 3 Compound: Gambogic acid	Cytotoxicity against human Raji cells Cytotoxicity against human Raji cells	[PMID: 25316317]
SMMC-7721	IC₅₀	1.13 3 Compound: 1, GA	Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay	[PMID: 22153338]
MCF7	IC₅₀	0.5 3 Compound: GA	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 20338759]
SGC-7901	IC₅₀	1.47 3 Compound: GA	Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay	[PMID: 20338759]
SMMC-7721	IC₅₀	3.2 3 Compound: 1, GA	Antiproliferative activity against human SMMC7721 cells after 24 hrs by MTT assay Antiproliferative activity against human SMMC7721 cells after 24 hrs by MTT assay	[PMID: 21334116]
MCF7	IC₅₀	1.25 3 Compound: 1; GA	Antiproliferative activity against human MCF7 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
SK-BR-3	GI₅₀	0.8 3 Compound: 1	Antiproliferative activity against human SKBR3 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human SKBR3 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
SNU-398	GI₅₀	0.2 3 Compound: 1, GA	Growth inhibition of human SNU398 cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human SNU398 cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
MCF7	IC₅₀	2.19 3 Compound: 1, GA	Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 21334116]
SK-BR-3	IC₅₀	0.89 3 Compound: 1; GA	Antiproliferative activity against human SK-BR-3 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human SK-BR-3 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
SNU-398	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human SNU398 cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human SNU398 cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
MDA-MB-231	IC₅₀	1.33 3 Compound: GA	Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
T47D	GI₅₀	0.2 3 Compound: 1, GA	Growth inhibition of human T47D cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human T47D cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
SK-OV-3	IC₅₀	3.06 3 Compound: gambogic acid, GA	Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay	[PMID: 18996626]
MKN-45	IC₅₀	4.27 3 Compound: GA	Antiproliferative activity against human MKN45 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MKN45 cells incubated for 72 hrs by MTT assay	[PMID: 31047774]
SMMC-7721	IC₅₀	3.2 3 Compound: 1, GA	Antiproliferative activity against human SMMC7721 cells after 24 hrs by MTT assay Antiproliferative activity against human SMMC7721 cells after 24 hrs by MTT assay	[PMID: 21334116]
T47D	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human T47D cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human T47D cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
PC-3	IC₅₀	1.19 3 Compound: 1; GA	Antiproliferative activity against human PC3 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human PC3 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
SMMC-7721	IC₅₀	1.13 3 Compound: 1, GA	Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay	[PMID: 22153338]
T98G	IC₅₀	≤ 300 1 Compound: GA	Antiproliferative activity against human T98G cells after 24 hrs by EZ-Tox assay Antiproliferative activity against human T98G cells after 24 hrs by EZ-Tox assay	[PMID: 26631318]
Raji	EC₅₀	0.24 3 Compound: Gambogic acid	Cytotoxicity against human Raji cells Cytotoxicity against human Raji cells	[PMID: 25316317]
SNU-398	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human SNU398 cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human SNU398 cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
U-251	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human U251 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human U251 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
SGC-7901	IC₅₀	1.47 3 Compound: GA	Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay	[PMID: 20338759]
U-251	IC₅₀	2.56 3 Compound: GA	Cytotoxicity against human U251 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human U251 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
SNU-398	GI₅₀	0.2 3 Compound: 1, GA	Growth inhibition of human SNU398 cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human SNU398 cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
T47D	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human T47D cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human T47D cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
U-251	IC₅₀	2.56 3 Compound: 1, GA	Cytotoxicity against human U251 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U251 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
SK-BR-3	GI₅₀	0.8 3 Compound: 1	Antiproliferative activity against human SKBR3 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay Antiproliferative activity against human SKBR3 cells assessed as inhibition of cell growth after 72 hrs by cell titer 96 assay	[PMID: 21486005]
SK-BR-3	IC₅₀	0.89 3 Compound: 1; GA	Antiproliferative activity against human SK-BR-3 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human SK-BR-3 cells assessed as growth inhibition by MTT assay	[PMID: 27527413]
U-251	IC₅₀	2.56 3 Compound: GA	Antiproliferative activity against human U251 cells after 24 hrs by MTT assay Antiproliferative activity against human U251 cells after 24 hrs by MTT assay	[PMID: 22472167]
T47D	GI₅₀	0.2 3 Compound: 1, GA	Growth inhibition of human T47D cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human T47D cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
T98G	IC₅₀	<= 300 1 Compound: GA	Antiproliferative activity against human T98G cells after 24 hrs by EZ-Tox assay Antiproliferative activity against human T98G cells after 24 hrs by EZ-Tox assay	[PMID: 26631318]
SK-OV-3	IC₅₀	3.06 3 Compound: gambogic acid, GA	Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay	[PMID: 18996626]
SMMC-7721	IC₅₀	1.13 3 Compound: 1, GA	Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay	[PMID: 22153338]
U-251	IC₅₀	2.56 3 Compound: GA	Antiproliferative activity against human U251 cells after 24 hrs by MTT assay Antiproliferative activity against human U251 cells after 24 hrs by MTT assay	[PMID: 22472167]
SMMC-7721	IC₅₀	3.2 3 Compound: 1, GA	Antiproliferative activity against human SMMC7721 cells after 24 hrs by MTT assay Antiproliferative activity against human SMMC7721 cells after 24 hrs by MTT assay	[PMID: 21334116]
U-251	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human U251 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human U251 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
SNU-398	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human SNU398 cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human SNU398 cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
U-251	IC₅₀	2.56 3 Compound: 1, GA	Cytotoxicity against human U251 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U251 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
SNU-398	GI₅₀	0.2 3 Compound: 1, GA	Growth inhibition of human SNU398 cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human SNU398 cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
U-251	IC₅₀	2.56 3 Compound: GA	Cytotoxicity against human U251 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human U251 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]
T47D	EC₅₀	0.7 3 Compound: 1, GA	Induction of apoptosis in human T47D cells assessed as caspase 3 activation after 24 hrs Induction of apoptosis in human T47D cells assessed as caspase 3 activation after 24 hrs	[PMID: 18337106]
T47D	GI₅₀	0.2 3 Compound: 1, GA	Growth inhibition of human T47D cells after 48 hrs by CellTiter-Glo assay Growth inhibition of human T47D cells after 48 hrs by CellTiter-Glo assay	[PMID: 18337106]
T98G	IC₅₀	<= 300 1 Compound: GA	Antiproliferative activity against human T98G cells after 24 hrs by EZ-Tox assay Antiproliferative activity against human T98G cells after 24 hrs by EZ-Tox assay	[PMID: 26631318]
U-251	IC₅₀	0.29 3 Compound: Gambogic acid	Antiproliferative activity against human U251 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human U251 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31546197]
U-251	IC₅₀	2.56 3 Compound: 1, GA	Cytotoxicity against human U251 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U251 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23167526]
U-251	IC₅₀	2.56 3 Compound: GA	Antiproliferative activity against human U251 cells after 24 hrs by MTT assay Antiproliferative activity against human U251 cells after 24 hrs by MTT assay	[PMID: 22472167]
U-251	IC₅₀	2.56 3 Compound: GA	Cytotoxicity against human U251 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human U251 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 25958244]

In Vitro

Gambogic Acid (Beta-Guttiferrin) is a medicinal compound derived from the gamboges resin of the tree, Garcinia hanburyi. Gambogic Acid has documented cytotoxic activity against tumor cell lines in culture, with concentrations required for killing 50% of cells (LD₅₀ of ~1 μM). The activity of Gambogic Acid against the 6 human anti-apoptotic Bcl-2-family proteins is contrasted, using FPAs. Gambogic Acid displaces to various extents FITC-BH3 peptide binding to all 6 proteins, with apparent IC₅₀ 1.47 μM for Bcl-X_L, 1.21 μM for Bcl-2, 2.02 μM for Bcl-W, 0.66 μM for Bcl-B, 1.06 μM for Bfl-1, and 0.79 μM for Mcl-1^[1]. The growth inhibitory effects of Gambogic Acid or Cisplatin (CDDP) on A549, NCI-H460, and NCI-H1299 cells are assessed by the MTT assay after 48?h exposure. A concentration-dependent inhibition of cell growth is observed with Gambogic Acid and CDDP, with IC₅₀s of 3.56±0.36 and 21.88±3.21?μM for A549 cells, 4.05±0.51 and 25.76±4.03?μM for NCI-H460 cells, and 1.12±0.31?μM and 25.21±4.38?μM for NCI-H1299 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

To further investigate whether Gambogic Acid (Beta-Guttiferrin) synergises CDDP against tumour growth in vivo, A549 tumors are implanted in SCID mice. When mice are treated with CDDP combined with Gambogic Acid, the tumor inhibition rate is 69.3%, whereas those of mice treated with CDDP and GA alone are 57.2% and 29.0%, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

628.75

Fòrmula

C₃₈H₄₄O₈

No. CAS

2752-65-0

Appearance

Solid

Color

Yellow to orange

SMILES

O=C(O)/C(C)=C\C[C@@]1(C2=O)OC(C)(C)[C@@](C[C@@]2([H])C=C34)([H])[C@]31OC5=C(C(O)=C(C=C[C@](CC/C=C(C)/C)(C)O6)C6=C5C/C=C(C)\C)C4=O

Structure Classification

Others

Initial Source

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 50 mg/mL (79.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5905 mL	7.9523 mL	15.9046 mL
5 mM	0.3181 mL	1.5905 mL	3.1809 mL
10 mM	0.1590 mL	0.7952 mL	1.5905 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 98.34%

Select Batch:

Ficha de datos (284 KB) SDS (479 KB)

COA (257 KB) HNMR (290 KB) RP-HPLC (260 KB) MS (69 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Zhai D, et al. Gambogic acid is an antagonist of antiapoptotic Bcl-2 family proteins. Mol Cancer Ther. 2008 Jun;7(6):1639-46. [Content Brief]

[2]. Wang LH, et al. Gambogic acid synergistically potentiates cisplatin-induced apoptosis in non-small-cell lung cancer through suppressing NF-κB and MAPK/HO-1 signalling. Br J Cancer. 2014 Jan 21;110(2):341-52. [Content Brief]

Ensayo celular ^[2]	The in vitro cell viability effects of Gambogic Acid, CDDP alone, or combined treatments are determined by MTT assay. The cells (2×10⁴ cells per mL) are seeded into 96-well culture plates. After overnight incubation, A549 cells are treated with Gambogic Acid (0.44, 0.88, 1.75, 3.5, 7, 10.5 and 14 μM); NCI-H460 cells are treated with Gambogic Acid (0.5, 1, 2, 4, 8, 12 and 16 μM); NCI-H1299 cells are treated with Gambogic Acid (0.125, 0.25, 0.5, 1, 2 and 4 μM). For the combined treatment in NSCLC cells, three sequences are tested: (a) Gambogic Acid followed by CDDP cells are exposed to Gambogic Acid for 48 h, and then after washout of Gambogic Acid, cells are treated with CDDP for an additional 48 h; (b) CDDP followed by Gambogic Acid cells are exposed to CDDP for 48 h, and then after washout of CDDP, cells are treated with Gambogic Acid for an additional 48 h; and (c) concurrent treatment cells are exposed to both Gambogic Acid and ADM for 48 h. The nature of the drug interaction is analysed by using the combination index (CI)^[2]. MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
Administraciòn de animales ^[2]	Mice^[2] To determine the in vivo antitumour activity of Gambogic Acid combined with CDDP, viable A549 cells (5×10⁶/100 μL PBS per mouse) are subcutaneously injected into the right flank of 7- to 8-week-old male SCID mice. When the average tumor volume reach 100 mm³, the mice are randomly divided into four treatment groups, including control (saline only, n=5), Gambogic Acid (3.0 mg/kg per 2 days, intravenously; n=6), CDDP (4 mg/kg per week, intravenously; n=6), and sequential combination (CDDP treatment one day before Gambogic Acid treatment, n=6). CDDP (4 mg/kg, weekly) is generally administered at doses less than the maximum-tolerated dose in an attempt to allow any additive effects of combination treatment with platinum-based agents and Gambogic Acid to be more easily detected. Tumor size is measured once every 2 days with a calipre. Body weight is recorded once every 2 days. After 14 days, the mice are killed and the tumors are excised and stored at -80 °C until further analysis. MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
Referencias	[1]. Zhai D, et al. Gambogic acid is an antagonist of antiapoptotic Bcl-2 family proteins. Mol Cancer Ther. 2008 Jun;7(6):1639-46. [Content Brief] [2]. Wang LH, et al. Gambogic acid synergistically potentiates cisplatin-induced apoptosis in non-small-cell lung cancer through suppressing NF-κB and MAPK/HO-1 signalling. Br J Cancer. 2014 Jan 21;110(2):341-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5905 mL	7.9523 mL	15.9046 mL	39.7614 mL
	5 mM	0.3181 mL	1.5905 mL	3.1809 mL	7.9523 mL
	10 mM	0.1590 mL	0.7952 mL	1.5905 mL	3.9761 mL
	15 mM	0.1060 mL	0.5302 mL	1.0603 mL	2.6508 mL
	20 mM	0.0795 mL	0.3976 mL	0.7952 mL	1.9881 mL
	25 mM	0.0636 mL	0.3181 mL	0.6362 mL	1.5905 mL
	30 mM	0.0530 mL	0.2651 mL	0.5302 mL	1.3254 mL
	40 mM	0.0398 mL	0.1988 mL	0.3976 mL	0.9940 mL
	50 mM	0.0318 mL	0.1590 mL	0.3181 mL	0.7952 mL
	60 mM	0.0265 mL	0.1325 mL	0.2651 mL	0.6627 mL

Gambogic Acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gambogic Acid2752-65-0Beta-GuttiferrinBcl-2 FamilyAutophagyInhibitorinhibitorinhibit

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Nombre del producto			Requested Quantity *
Nombre del solicitante *			Saludo
Direcciòn del E-mail *			Número de teléfono *
Department			Nombre de la Organizaciòn *
City			Provincia
Country or Region *
Observaciones

Nombre del producto			Lot #
Nombre del solicitante *			Direcciòn del E-mail *
Número de teléfono			Department *
Nombre de la Organizaciòn *			Country or Region *
Observaciones

Name
E-mail *

	Sorry, but the email address you supplied was invalid.

Gambogic Acid (Synonyms: Beta-Guttiferrin)

Revisión del cliente

Other Forms of Gambogic Acid:

Gambogic Acid Related Antibodies

6 Publications Citing Use of MCE Gambogic Acid

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Bcl-2 Family:

Actividad biológica

Protocolo

Pureza y Documentación

Referencias

Revisión del cliente

Gambogic Acid Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Gambogic Acid Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report