1. GPCR/G Protein
  2. mGluR

L-APB (Synonyms: L-AP 4)

Cat. No.: HY-100781A Purity: >99.0%
Handling Instructions

L-APB is a potent and specific agonist for the group III mGluRs, with EC50s of 0.13, 0.29, 1.0, 249 μM for mGlu4, mGlu8, mGlu6 and mGlu7 receptors, respectively.

For research use only. We do not sell to patients.

L-APB Chemical Structure

L-APB Chemical Structure

CAS No. : 23052-81-5

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1 mg USD 90 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 Get quote
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  • Biological Activity

  • Protocol

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  • References

Description

L-APB is a potent and specific agonist for the group III mGluRs, with EC50s of 0.13, 0.29, 1.0, 249 μM for mGlu4, mGlu8, mGlu6 and mGlu7 receptors, respectively.

IC50 & Target

mGlu4

0.13 μM (EC50)

mGlu8

0.29 μM (EC50)

mGlu6

1.0 μM (EC50)

mGlu7

249 μM (EC50)

In Vivo

Paw withdrawal threshold in response to application of von Frey filaments before spinal nerve ligation is 22.6±2.4 g. The mechanical threshold decreases significantly (2.3±0.5 g, P<0.05) within 10 days after nerve ligation and remains stable for at least 8 weeks. Intrathecal injection of 5 to 30 μg of L-APB significantly increases the paw withdrawal threshold in response to application of von Frey filaments in eight nerve-ligated rats in a dose-dependent manner. The maximal effect of L-APB appears within 45 min and gradually subsided in 120 min following intrathecal administration[2].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.4615 mL 27.3075 mL 54.6150 mL
5 mM 1.0923 mL 5.4615 mL 10.9230 mL
10 mM 0.5461 mL 2.7307 mL 5.4615 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[2]

Rats[2]
The effect of L-APB (L-AP4: 5, 10, and 50 μM) on identified ascending dorsal horn neurons is studied in 20 L5 and L6 spinal nerve ligated rats. L-APB, starting with the lowest concentration, is applied topically onto the exposed spinal cord. Five to 10 min following L-APB application, the response of neurons to graded mechanical stimuli is re-examined. To ensure that the effect of L-APB on dorsal horn neurons is through group III mGluRs, the inhibitory effect of 50 μM L-APB is tested before and after topical application of 100 μM MAP4, a specific antagonist for group III mGluRs, in another 12 dorsal horn neurons. MAP4 is applied to the recording site 10 min before application of 50 μM L-APB. In addition, to determine whether topical application of L-APB affects ascending dorsal horn neurons in normal rats, the effect of 50 μM L-APB on spontaneous and evoked responses to graded mechanical stimuli is examined in 11 dorsal horn neurons from eight normal rats. Also, the effect of topical spinal application of 100 μM MAP4 alone on these cells is tested 15 to 20 min after washout of L-APB solution from the recording site[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

183.10

Formula

C₄H₁₀NO₅P

CAS No.

23052-81-5

SMILES

O=C(O)[[email protected]@H](N)CCP(O)(O)=O

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: >99.0%

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Product Name:
L-APB
Cat. No.:
HY-100781A
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L-APB

Cat. No.: HY-100781A