2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. L-Alanine

L-Alanine  (Synonyms: L-2-Aminopropionic acid)

Cat. No.: HY-N0229 Purity: 99.93%
COA
SDS
Handling Instructions Technical Support

L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.

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L-Alanine

L-Alanine 화학구조

CAS No. : 56-41-7

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in Water 해외재고보유
Solid
500 mg 해외재고보유
5 g 해외재고보유
10 g   견적 받기  
50 g   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 4 publication(s) in Google Scholar

Other Forms of L-Alanine:

L-Alanine Related Antibodies

Top Publications Citing Use of Products

View All Endogenous Metabolite Isoform Specific Products:

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Human Endogenous Metabolite Microbial Metabolite

  • Biological Activity

  • Protocol

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

The viability of both hiPSCs, 201B7 cells and ehiPSCs decrease with an increase in L-Alanine concentration, and reach 7.5±1.3% and 3.7±0.7% respectively at 1.2 M of L-Alanine. On the other hand, no decrease in the viability of hFBs and hSkMCs are observed. Although the viability of iCMs slightly decreases along with the increase of the L-Alanine concentration, viability of iCMs at 1.2 M concentration of L-Alanine, 49.4±6.9%, is significantly higher than that of undifferentiated iPSCs, 201B7 cells and ehiPSCs (p< 0.01). The viability of hiPSCs, 201B7 cells and ehiPSCs, drastically decrease even after 2 or 4 h treatment. In contrast, the viability of hFBs fails to decrease at 1, 2, and 4 h and shows a small decrease at 24 h treatment. The viability of 201B7 cells in suspension culture decreases to 11.8±6.0% following treatment with 1.2 M L-Alanine for 2 h, whereas that of hFBs is 72.9±14.2%[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Alanine Related Antibodies
분자량

89.09

화학식

C3H7NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](C)C(O)=O
Structure Classification
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
용액&용해도
In Vitro: 

H2O : 50 mg/mL (561.23 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 11.2246 mL 56.1230 mL 112.2460 mL
5 mM 2.2449 mL 11.2246 mL 22.4492 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (561.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
순도&문서

Purity: 99.93%

SDS (393 KB)

COA (250 KB) HNMR (105 KB) RP-HPLC (242 KB)

Handling Instructions (2659 KB)
References
Cell Assay 

To investigate the selective removal of hiPSCs from differentiated cells by the high L-Alanine medium, two types of hiPSCs, 201B7 hiPSCs (201B7 cells) and an hiPSC line derived by episomal system (ehiPSCs) are used, along with normal human dermal fibroblasts (hFBs), human skeletal muscle cells (hSkMCs) and hiPSC-derived cardiomyocytes (iCMs) as differentiated cells. The cells are incubated in a medium supplemented with L-Alanine at various concentrations (0-1.2 M) or treatment times (1-24 h). The medium is replaced with a normal medium and the relative cell viability is measured after 24 h[1].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 11.2246 mL 56.1230 mL 112.2460 mL 280.6151 mL
5 mM 2.2449 mL 11.2246 mL 22.4492 mL 56.1230 mL
10 mM 1.1225 mL 5.6123 mL 11.2246 mL 28.0615 mL
15 mM 0.7483 mL 3.7415 mL 7.4831 mL 18.7077 mL
20 mM 0.5612 mL 2.8062 mL 5.6123 mL 14.0308 mL
25 mM 0.4490 mL 2.2449 mL 4.4898 mL 11.2246 mL
30 mM 0.3742 mL 1.8708 mL 3.7415 mL 9.3538 mL
40 mM 0.2806 mL 1.4031 mL 2.8062 mL 7.0154 mL
50 mM 0.2245 mL 1.1225 mL 2.2449 mL 5.6123 mL
60 mM 0.1871 mL 0.9354 mL 1.8708 mL 4.6769 mL
80 mM 0.1403 mL 0.7015 mL 1.4031 mL 3.5077 mL
100 mM 0.1122 mL 0.5612 mL 1.1225 mL 2.8062 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Alanine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Alanine56-41-7L-2-Aminopropionic acidEndogenous MetaboliteInhibitorinhibitorinhibit

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상품명:
L-Alanine
Cat. No.:
HY-N0229
수량:
MCE Japan Authorized Agent:

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SAFETY DATA SHEET (SDS)

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